Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses
We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us to focally photostimulate these neurons, while me...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2016
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| Online Access: | http://hdl.handle.net/1721.1/101430 https://orcid.org/0000-0002-8021-277X |
| _version_ | 1826206582821617664 |
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| author | Kim, Jinsook Lee, Soojung Tsuda, Sachiko Zhang, Xuying Asrican, Brent Gloss, Bernd Feng, Guoping Augustine, George J. |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Kim, Jinsook Lee, Soojung Tsuda, Sachiko Zhang, Xuying Asrican, Brent Gloss, Bernd Feng, Guoping Augustine, George J. |
| author_sort | Kim, Jinsook |
| collection | MIT |
| description | We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us to focally photostimulate these neurons, while measuring resulting responses in postsynaptic Purkinje cells. This approach revealed that interneurons converge upon Purkinje cells over a broad area and that at least seven interneurons form functional synapses with a single Purkinje cell. The number of converging interneurons was reduced by treatment with gap junction blockers, revealing that electrical synapses between interneurons contribute substantially to the spatial convergence. Remarkably, gap junction blockers affected convergence in sagittal slices, but not in coronal slices, indicating a sagittal bias in electrical coupling between interneurons. We conclude that electrical synapse networks spatially coordinate interneurons in the cerebellum and may also serve this function in other brain regions. |
| first_indexed | 2024-09-23T13:34:53Z |
| format | Article |
| id | mit-1721.1/101430 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:34:53Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1014302022-10-01T15:51:26Z Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses Kim, Jinsook Lee, Soojung Tsuda, Sachiko Zhang, Xuying Asrican, Brent Gloss, Bernd Feng, Guoping Augustine, George J. Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Feng, Guoping We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us to focally photostimulate these neurons, while measuring resulting responses in postsynaptic Purkinje cells. This approach revealed that interneurons converge upon Purkinje cells over a broad area and that at least seven interneurons form functional synapses with a single Purkinje cell. The number of converging interneurons was reduced by treatment with gap junction blockers, revealing that electrical synapses between interneurons contribute substantially to the spatial convergence. Remarkably, gap junction blockers affected convergence in sagittal slices, but not in coronal slices, indicating a sagittal bias in electrical coupling between interneurons. We conclude that electrical synapse networks spatially coordinate interneurons in the cerebellum and may also serve this function in other brain regions. Singapore. National Research Foundation (CRP Grant) Korea (South). Ministry of Education, Science and Technology (National Research Foundation of Korea Grant WCI 2009-003) 2016-03-03T03:11:38Z 2016-03-03T03:11:38Z 2014-05 2014-02 Article http://purl.org/eprint/type/JournalArticle 22111247 http://hdl.handle.net/1721.1/101430 Kim, Jinsook, Soojung Lee, Sachiko Tsuda, Xuying Zhang, Brent Asrican, Bernd Gloss, Guoping Feng, and George J. Augustine. “Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses.” Cell Reports 7, no. 5 (June 2014): 1601–1613. https://orcid.org/0000-0002-8021-277X en_US http://dx.doi.org/10.1016/j.celrep.2014.04.047 Cell Reports Creative Commons Attribution http://creativecommons.org/licenses/by/3.0/ application/pdf Elsevier Elsevier |
| spellingShingle | Kim, Jinsook Lee, Soojung Tsuda, Sachiko Zhang, Xuying Asrican, Brent Gloss, Bernd Feng, Guoping Augustine, George J. Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses |
| title | Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses |
| title_full | Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses |
| title_fullStr | Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses |
| title_full_unstemmed | Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses |
| title_short | Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses |
| title_sort | optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses |
| url | http://hdl.handle.net/1721.1/101430 https://orcid.org/0000-0002-8021-277X |
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