Site-Directed Mutagenesis to Improve Sensitivity of a Synthetic Two-Component Signaling System

Two-component signaling (2CS) systems enable bacterial cells to respond to changes in their local environment, often using a membrane-bound sensor protein and a cytoplasmic responder protein to regulate gene expression. Previous work has shown that Escherichia coli’s natural EnvZ/OmpR 2CS could be m...

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Bibliographic Details
Main Authors: Shehata, Laila, Kuldell, Natalie, Olshefsky, Audrey E.
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: Public Library of Science 2016
Online Access:http://hdl.handle.net/1721.1/101670
https://orcid.org/0000-0001-9483-1291