Protein-targeted corona phase molecular recognition
Corona phase molecular recognition (CoPhMoRe) uses a heteropolymer adsorbed onto and templated by a nanoparticle surface to recognize a specific target analyte. This method has not yet been extended to macromolecular analytes, including proteins. Herein we develop a variant of a CoPhMoRe screening p...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2016
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| Online Access: | http://hdl.handle.net/1721.1/101732 https://orcid.org/0000-0001-9785-8297 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0001-6031-2338 https://orcid.org/0000-0003-2944-808X |
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| author | Iverson, Nicole M. Ahn, Jiyoung Nelson, Justin Theodore Landry, Markita P. Kruss, Sebastian Strano, Michael S. Bisker Raviv, Gili Hana Dong, Juyao-Ivy Park, Hoyoung Daniel |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Iverson, Nicole M. Ahn, Jiyoung Nelson, Justin Theodore Landry, Markita P. Kruss, Sebastian Strano, Michael S. Bisker Raviv, Gili Hana Dong, Juyao-Ivy Park, Hoyoung Daniel |
| author_sort | Iverson, Nicole M. |
| collection | MIT |
| description | Corona phase molecular recognition (CoPhMoRe) uses a heteropolymer adsorbed onto and templated by a nanoparticle surface to recognize a specific target analyte. This method has not yet been extended to macromolecular analytes, including proteins. Herein we develop a variant of a CoPhMoRe screening procedure of single-walled carbon nanotubes (SWCNT) and use it against a panel of human blood proteins, revealing a specific corona phase that recognizes fibrinogen with high selectivity. In response to fibrinogen binding, SWCNT fluorescence decreases by >80% at saturation. Sequential binding of the three fibrinogen nodules is suggested by selective fluorescence quenching by isolated sub-domains and validated by the quenching kinetics. The fibrinogen recognition also occurs in serum environment, at the clinically relevant fibrinogen concentrations in the human blood. These results open new avenues for synthetic, non-biological antibody analogues that recognize biological macromolecules, and hold great promise for medical and clinical applications. |
| first_indexed | 2024-09-23T09:51:21Z |
| format | Article |
| id | mit-1721.1/101732 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:51:21Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1017322022-09-30T17:16:16Z Protein-targeted corona phase molecular recognition Iverson, Nicole M. Ahn, Jiyoung Nelson, Justin Theodore Landry, Markita P. Kruss, Sebastian Strano, Michael S. Bisker Raviv, Gili Hana Dong, Juyao-Ivy Park, Hoyoung Daniel Massachusetts Institute of Technology. Department of Chemical Engineering Bisker Raviv, Gili Hana Dong, Juyao-Ivy Park, Hoyoung Daniel Ahn, Jiyoung Nelson, Justin Theodore Strano, Michael S. Corona phase molecular recognition (CoPhMoRe) uses a heteropolymer adsorbed onto and templated by a nanoparticle surface to recognize a specific target analyte. This method has not yet been extended to macromolecular analytes, including proteins. Herein we develop a variant of a CoPhMoRe screening procedure of single-walled carbon nanotubes (SWCNT) and use it against a panel of human blood proteins, revealing a specific corona phase that recognizes fibrinogen with high selectivity. In response to fibrinogen binding, SWCNT fluorescence decreases by >80% at saturation. Sequential binding of the three fibrinogen nodules is suggested by selective fluorescence quenching by isolated sub-domains and validated by the quenching kinetics. The fibrinogen recognition also occurs in serum environment, at the clinically relevant fibrinogen concentrations in the human blood. These results open new avenues for synthetic, non-biological antibody analogues that recognize biological macromolecules, and hold great promise for medical and clinical applications. Juvenile Diabetes Research Foundation International MIT-Technion Fellowship 2016-03-17T01:20:47Z 2016-03-17T01:20:47Z 2016-01 2015-01 Article http://purl.org/eprint/type/JournalArticle 2041-1723 http://hdl.handle.net/1721.1/101732 Bisker, Gili, Juyao Dong, Hoyoung D. Park, Nicole M. Iverson, Jiyoung Ahn, Justin T. Nelson, Markita P. Landry, Sebastian Kruss, and Michael S. Strano. “Protein-Targeted Corona Phase Molecular Recognition.” Nat Comms 7 (January 8, 2016): 10241. https://orcid.org/0000-0001-9785-8297 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0001-6031-2338 https://orcid.org/0000-0003-2944-808X en_US http://dx.doi.org/10.1038/ncomms10241 Nature Communications Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Iverson, Nicole M. Ahn, Jiyoung Nelson, Justin Theodore Landry, Markita P. Kruss, Sebastian Strano, Michael S. Bisker Raviv, Gili Hana Dong, Juyao-Ivy Park, Hoyoung Daniel Protein-targeted corona phase molecular recognition |
| title | Protein-targeted corona phase molecular recognition |
| title_full | Protein-targeted corona phase molecular recognition |
| title_fullStr | Protein-targeted corona phase molecular recognition |
| title_full_unstemmed | Protein-targeted corona phase molecular recognition |
| title_short | Protein-targeted corona phase molecular recognition |
| title_sort | protein targeted corona phase molecular recognition |
| url | http://hdl.handle.net/1721.1/101732 https://orcid.org/0000-0001-9785-8297 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0001-6031-2338 https://orcid.org/0000-0003-2944-808X |
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