MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation
The Escherichia coli SMC complex, MukBEF, forms clusters of molecules that interact with the decatenase topisomerase IV and which are normally associated with the chromosome replication origin region (ori). Here we demonstrate an additional ATP-hydrolysis-dependent association of MukBEF with the rep...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2016
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| Online Access: | http://hdl.handle.net/1721.1/101744 https://orcid.org/0000-0002-6807-6576 |
| _version_ | 1826197446486654976 |
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| author | Nolivos, Sophie Upton, Amy L. Badrinarayanan, Anjana Zawadzka, Katarzyna Wiktor, Jakub Gill, Amber Arciszewska, Lidia Nicolas, Emilien Sherratt, David Muller, Julius |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Nolivos, Sophie Upton, Amy L. Badrinarayanan, Anjana Zawadzka, Katarzyna Wiktor, Jakub Gill, Amber Arciszewska, Lidia Nicolas, Emilien Sherratt, David Muller, Julius |
| author_sort | Nolivos, Sophie |
| collection | MIT |
| description | The Escherichia coli SMC complex, MukBEF, forms clusters of molecules that interact with the decatenase topisomerase IV and which are normally associated with the chromosome replication origin region (ori). Here we demonstrate an additional ATP-hydrolysis-dependent association of MukBEF with the replication termination region (ter). Consistent with this, MukBEF interacts with MatP, which binds matS sites in ter. MatP displaces wild-type MukBEF complexes from ter, thereby facilitating their association with ori, and limiting the availability of topoisomerase IV (TopoIV) at ter. Displacement of MukBEF is impaired when MukB ATP hydrolysis is compromised and when MatP is absent, leading to a stable association of ter and MukBEF. Impairing the TopoIV-MukBEF interaction delays sister ter segregation in cells lacking MatP. We propose that the interplay between MukBEF and MatP directs chromosome organization in relation to MukBEF clusters and associated topisomerase IV, thereby ensuring timely chromosome unlinking and segregation. |
| first_indexed | 2024-09-23T10:47:48Z |
| format | Article |
| id | mit-1721.1/101744 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:47:48Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1017442022-09-27T15:05:29Z MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation Nolivos, Sophie Upton, Amy L. Badrinarayanan, Anjana Zawadzka, Katarzyna Wiktor, Jakub Gill, Amber Arciszewska, Lidia Nicolas, Emilien Sherratt, David Muller, Julius Massachusetts Institute of Technology. Department of Biology Badrinarayanan, Anjana The Escherichia coli SMC complex, MukBEF, forms clusters of molecules that interact with the decatenase topisomerase IV and which are normally associated with the chromosome replication origin region (ori). Here we demonstrate an additional ATP-hydrolysis-dependent association of MukBEF with the replication termination region (ter). Consistent with this, MukBEF interacts with MatP, which binds matS sites in ter. MatP displaces wild-type MukBEF complexes from ter, thereby facilitating their association with ori, and limiting the availability of topoisomerase IV (TopoIV) at ter. Displacement of MukBEF is impaired when MukB ATP hydrolysis is compromised and when MatP is absent, leading to a stable association of ter and MukBEF. Impairing the TopoIV-MukBEF interaction delays sister ter segregation in cells lacking MatP. We propose that the interplay between MukBEF and MatP directs chromosome organization in relation to MukBEF clusters and associated topisomerase IV, thereby ensuring timely chromosome unlinking and segregation. 2016-03-18T17:14:33Z 2016-03-18T17:14:33Z 2016-01 2015-08 Article http://purl.org/eprint/type/JournalArticle 2041-1723 http://hdl.handle.net/1721.1/101744 Nolivos, Sophie, Amy L. Upton, Anjana Badrinarayanan, Julius Müller, Katarzyna Zawadzka, Jakub Wiktor, Amber Gill, Lidia Arciszewska, Emilien Nicolas, and David Sherratt. “MatP Regulates the Coordinated Action of Topoisomerase IV and MukBEF in Chromosome Segregation.” Nat Comms 7 (January 28, 2016): 10466. https://orcid.org/0000-0002-6807-6576 en_US http://dx.doi.org/10.1038/ncomms10466 Nature Communications Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Nolivos, Sophie Upton, Amy L. Badrinarayanan, Anjana Zawadzka, Katarzyna Wiktor, Jakub Gill, Amber Arciszewska, Lidia Nicolas, Emilien Sherratt, David Muller, Julius MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
| title | MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
| title_full | MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
| title_fullStr | MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
| title_full_unstemmed | MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
| title_short | MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
| title_sort | matp regulates the coordinated action of topoisomerase iv and mukbef in chromosome segregation |
| url | http://hdl.handle.net/1721.1/101744 https://orcid.org/0000-0002-6807-6576 |
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