Three-Dimensional Imaging of Electrospun Fiber Mats Using Confocal Laser Scanning Microscopy and Digital Image Analysis
Confocal laser scanning microscopy with fluorescent markers and index matching has been used to collect three-dimensional (3D) digitized images of electrospun fiber mats and of a borosilicate glass fiber material. By embedding the fluorescent dye in either the material component (fibers) or pore spa...
| Main Authors: | , , |
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| Format: | Article |
| Language: | en_US |
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Springer-Verlag
2016
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| Online Access: | http://hdl.handle.net/1721.1/102315 https://orcid.org/0000-0001-8137-1732 |
| _version_ | 1811082666801364992 |
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| author | Choong, Looh Tchuin (Simon) Yi, Peng Rutledge, Gregory C. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Choong, Looh Tchuin (Simon) Yi, Peng Rutledge, Gregory C. |
| author_sort | Choong, Looh Tchuin (Simon) |
| collection | MIT |
| description | Confocal laser scanning microscopy with fluorescent markers and index matching has been used to collect three-dimensional (3D) digitized images of electrospun fiber mats and of a borosilicate glass fiber material. By embedding the fluorescent dye in either the material component (fibers) or pore space component (the index-matching fluid), acquisitions of both positive and negative images of the porous fibrous materials are demonstrated. Image analysis techniques are then applied to the 3D reconstructions of the fibrous materials to extract important morphological characteristics such as porosity, specific surface area, distributions of fiber diameter and of pore diameter, and fiber orientation distribution; the results are compared with other experimental measurements where available. The topology of the pore space is quantified for an electrospun mat for the first time using the Euler-Poincare characteristic. Finally, a method is presented for subdividing the pore space into a network of cavities and the gates that interconnect them, by which the network structure of the pore space in these electrospun mats is determined. |
| first_indexed | 2024-09-23T12:06:59Z |
| format | Article |
| id | mit-1721.1/102315 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:06:59Z |
| publishDate | 2016 |
| publisher | Springer-Verlag |
| record_format | dspace |
| spelling | mit-1721.1/1023152022-10-01T08:18:45Z Three-Dimensional Imaging of Electrospun Fiber Mats Using Confocal Laser Scanning Microscopy and Digital Image Analysis Choong, Looh Tchuin (Simon) Yi, Peng Rutledge, Gregory C. Massachusetts Institute of Technology. Department of Chemical Engineering Rutledge, Gregory C. Choong, Looh Tchuin (Simon) Yi, Peng Rutledge, Gregory C. Confocal laser scanning microscopy with fluorescent markers and index matching has been used to collect three-dimensional (3D) digitized images of electrospun fiber mats and of a borosilicate glass fiber material. By embedding the fluorescent dye in either the material component (fibers) or pore space component (the index-matching fluid), acquisitions of both positive and negative images of the porous fibrous materials are demonstrated. Image analysis techniques are then applied to the 3D reconstructions of the fibrous materials to extract important morphological characteristics such as porosity, specific surface area, distributions of fiber diameter and of pore diameter, and fiber orientation distribution; the results are compared with other experimental measurements where available. The topology of the pore space is quantified for an electrospun mat for the first time using the Euler-Poincare characteristic. Finally, a method is presented for subdividing the pore space into a network of cavities and the gates that interconnect them, by which the network structure of the pore space in these electrospun mats is determined. EMD Millipore Corporation 2016-04-28T14:06:00Z 2016-04-28T14:06:00Z 2015-01 2014-09 Article http://purl.org/eprint/type/JournalArticle 0022-2461 1573-4803 http://hdl.handle.net/1721.1/102315 Choong, Looh Tchuin, Peng Yi, and Gregory C. Rutledge. "Three-Dimensional Imaging of Electrospun Fiber Mats Using Confocal Laser Scanning Microscopy and Digital Image Analysis." Journal of Materials Science 50(8): 3014-3030 (April 2015). https://orcid.org/0000-0001-8137-1732 en_US http://dx.doi.org/10.1007/s10853-015-8834-2 Journal of Materials Science Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer-Verlag Prof. Rutledge via Erja Kajosalo |
| spellingShingle | Choong, Looh Tchuin (Simon) Yi, Peng Rutledge, Gregory C. Three-Dimensional Imaging of Electrospun Fiber Mats Using Confocal Laser Scanning Microscopy and Digital Image Analysis |
| title | Three-Dimensional Imaging of Electrospun Fiber Mats Using Confocal Laser Scanning Microscopy and Digital Image Analysis |
| title_full | Three-Dimensional Imaging of Electrospun Fiber Mats Using Confocal Laser Scanning Microscopy and Digital Image Analysis |
| title_fullStr | Three-Dimensional Imaging of Electrospun Fiber Mats Using Confocal Laser Scanning Microscopy and Digital Image Analysis |
| title_full_unstemmed | Three-Dimensional Imaging of Electrospun Fiber Mats Using Confocal Laser Scanning Microscopy and Digital Image Analysis |
| title_short | Three-Dimensional Imaging of Electrospun Fiber Mats Using Confocal Laser Scanning Microscopy and Digital Image Analysis |
| title_sort | three dimensional imaging of electrospun fiber mats using confocal laser scanning microscopy and digital image analysis |
| url | http://hdl.handle.net/1721.1/102315 https://orcid.org/0000-0001-8137-1732 |
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