A Ratiometric Sensor Using Single Chirality Near-Infrared Fluorescent Carbon Nanotubes: Application to In Vivo Monitoring
Advances in the separation and functionalization of single walled carbon nanotubes (SWCNT) by their electronic type have enabled the development of ratiometric fluorescent SWCNT sensors for the first time. Herein, single chirality SWCNT are independently functionalized to recognize either nitric oxi...
| Main Authors: | , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Wiley Blackwell
2016
|
| Online Access: | http://hdl.handle.net/1721.1/102316 https://orcid.org/0000-0002-5832-8522 https://orcid.org/0000-0002-6960-1985 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0001-6988-9096 https://orcid.org/0000-0002-5166-1410 |
| _version_ | 1826200073180020736 |
|---|---|
| author | Giraldo, Juan P. Jain, Rishabh M. Wong, Min Hao Iverson, Nicole M. Ben-Naim, Micha Strano, Michael S. Landry, Markita Patricia Kwak, Seonyeong |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Giraldo, Juan P. Jain, Rishabh M. Wong, Min Hao Iverson, Nicole M. Ben-Naim, Micha Strano, Michael S. Landry, Markita Patricia Kwak, Seonyeong |
| author_sort | Giraldo, Juan P. |
| collection | MIT |
| description | Advances in the separation and functionalization of single walled carbon nanotubes (SWCNT) by their electronic type have enabled the development of ratiometric fluorescent SWCNT sensors for the first time. Herein, single chirality SWCNT are independently functionalized to recognize either nitric oxide (NO), hydrogen peroxide (H[subscript 2]O[subscript 2]), or no analyte (remaining invariant) to create optical sensor responses from the ratio of distinct emission peaks. This ratiometric approach provides a measure of analyte concentration, invariant to the absolute intensity emitted from the sensors and hence, more stable to external noise and detection geometry. Two distinct ratiometric sensors are demonstrated: one version for H[subscript 2]O[subscript 2], the other for NO, each using 7,6 emission, and each containing an invariant 6,5 emission wavelength. To functionalize these sensors from SWCNT isolated from the gel separation technique, a method for rapid and efficient coating exchange of single chirality sodium dodecyl sulfate-SWCNT is introduced. As a proof of concept, spatial and temporal patterns of the ratio sensor response to H[subscript 2]O[subscript 2] and, separately, NO, are monitored in leaves of living plants in real time. This ratiometric optical sensing platform can enable the detection of trace analytes in complex environments such as strongly scattering media and biological tissues. |
| first_indexed | 2024-09-23T11:30:44Z |
| format | Article |
| id | mit-1721.1/102316 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:30:44Z |
| publishDate | 2016 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/1023162022-09-27T20:01:25Z A Ratiometric Sensor Using Single Chirality Near-Infrared Fluorescent Carbon Nanotubes: Application to In Vivo Monitoring Giraldo, Juan P. Jain, Rishabh M. Wong, Min Hao Iverson, Nicole M. Ben-Naim, Micha Strano, Michael S. Landry, Markita Patricia Kwak, Seonyeong Massachusetts Institute of Technology. Department of Chemical Engineering Strano, Michael S. Giraldo, Juan P. Landry, Markita Patricia Kwak, Seonyeong Jain, Rishabh M. Wong, Min Hao Iverson, Nicole M. Ben-Naim, Micha Strano, Michael S. Advances in the separation and functionalization of single walled carbon nanotubes (SWCNT) by their electronic type have enabled the development of ratiometric fluorescent SWCNT sensors for the first time. Herein, single chirality SWCNT are independently functionalized to recognize either nitric oxide (NO), hydrogen peroxide (H[subscript 2]O[subscript 2]), or no analyte (remaining invariant) to create optical sensor responses from the ratio of distinct emission peaks. This ratiometric approach provides a measure of analyte concentration, invariant to the absolute intensity emitted from the sensors and hence, more stable to external noise and detection geometry. Two distinct ratiometric sensors are demonstrated: one version for H[subscript 2]O[subscript 2], the other for NO, each using 7,6 emission, and each containing an invariant 6,5 emission wavelength. To functionalize these sensors from SWCNT isolated from the gel separation technique, a method for rapid and efficient coating exchange of single chirality sodium dodecyl sulfate-SWCNT is introduced. As a proof of concept, spatial and temporal patterns of the ratio sensor response to H[subscript 2]O[subscript 2] and, separately, NO, are monitored in leaves of living plants in real time. This ratiometric optical sensing platform can enable the detection of trace analytes in complex environments such as strongly scattering media and biological tissues. National Science Foundation (U.S.) (Grant 1213622) National Science Foundation (U.S.) (Postdoctoral Research Fellowship in Biology Award 1103600) National Science Foundation (U.S.) (Postdoctoral Research Fellowship in Biology Award 1306229) Burroughs Wellcome Fund (Grant 1013994) 2016-04-28T14:18:56Z 2016-04-28T14:18:56Z 2015-05 2015-02 Article http://purl.org/eprint/type/JournalArticle 16136810 1613-6829 http://hdl.handle.net/1721.1/102316 Giraldo, Juan P., Markita P. Landry, Seon-Yeong Kwak, Rishabh M. Jain, Min Hao Wong, Nicole M. Iverson, Micha Ben-Naim, and Michael S. Strano. “A Ratiometric Sensor Using Single Chirality Near-Infrared Fluorescent Carbon Nanotubes: Application to In Vivo Monitoring.” Small 11, no. 32 (May 15, 2015): 3973–3984. https://orcid.org/0000-0002-5832-8522 https://orcid.org/0000-0002-6960-1985 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0001-6988-9096 https://orcid.org/0000-0002-5166-1410 en_US http://dx.doi.org/10.1002/smll.201403276 Small Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell Prof. Strano via Erja Kajosalo |
| spellingShingle | Giraldo, Juan P. Jain, Rishabh M. Wong, Min Hao Iverson, Nicole M. Ben-Naim, Micha Strano, Michael S. Landry, Markita Patricia Kwak, Seonyeong A Ratiometric Sensor Using Single Chirality Near-Infrared Fluorescent Carbon Nanotubes: Application to In Vivo Monitoring |
| title | A Ratiometric Sensor Using Single Chirality Near-Infrared Fluorescent Carbon Nanotubes: Application to In Vivo Monitoring |
| title_full | A Ratiometric Sensor Using Single Chirality Near-Infrared Fluorescent Carbon Nanotubes: Application to In Vivo Monitoring |
| title_fullStr | A Ratiometric Sensor Using Single Chirality Near-Infrared Fluorescent Carbon Nanotubes: Application to In Vivo Monitoring |
| title_full_unstemmed | A Ratiometric Sensor Using Single Chirality Near-Infrared Fluorescent Carbon Nanotubes: Application to In Vivo Monitoring |
| title_short | A Ratiometric Sensor Using Single Chirality Near-Infrared Fluorescent Carbon Nanotubes: Application to In Vivo Monitoring |
| title_sort | ratiometric sensor using single chirality near infrared fluorescent carbon nanotubes application to in vivo monitoring |
| url | http://hdl.handle.net/1721.1/102316 https://orcid.org/0000-0002-5832-8522 https://orcid.org/0000-0002-6960-1985 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0001-6988-9096 https://orcid.org/0000-0002-5166-1410 |
| work_keys_str_mv | AT giraldojuanp aratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT jainrishabhm aratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT wongminhao aratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT iversonnicolem aratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT bennaimmicha aratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT stranomichaels aratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT landrymarkitapatricia aratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT kwakseonyeong aratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT giraldojuanp ratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT jainrishabhm ratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT wongminhao ratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT iversonnicolem ratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT bennaimmicha ratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT stranomichaels ratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT landrymarkitapatricia ratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring AT kwakseonyeong ratiometricsensorusingsinglechiralitynearinfraredfluorescentcarbonnanotubesapplicationtoinvivomonitoring |