Development of transgenic animals for optogenetic manipulation of mammalian nervous system function: Progress and prospects for behavioral neuroscience
Here we review the rapidly growing toolbox of transgenic mice and rats that exhibit functional expression of engineered opsins for neuronal activation and silencing with light. Collectively, these transgenic animals are enabling neuroscientists to access and manipulate the many diverse cell types in...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2016
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| Online Access: | http://hdl.handle.net/1721.1/102413 https://orcid.org/0000-0002-8021-277X |
| _version_ | 1826215888447078400 |
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| author | Feng, Guoping Ting, Jonathan Thomas |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Feng, Guoping Ting, Jonathan Thomas |
| author_sort | Feng, Guoping |
| collection | MIT |
| description | Here we review the rapidly growing toolbox of transgenic mice and rats that exhibit functional expression of engineered opsins for neuronal activation and silencing with light. Collectively, these transgenic animals are enabling neuroscientists to access and manipulate the many diverse cell types in the mammalian nervous system in order to probe synaptic and circuitry connectivity, function, and dysfunction. The availability of transgenic lines affords important advantages such as stable and heritable transgene expression patterns across experimental cohorts. As such, the use of transgenic lines precludes the need for other costly and labor-intensive procedures to achieve functional transgene expression in each individual experimental animal. This represents an important consideration when large cohorts of experimental animals are desirable as in many common behavioral assays. We describe the diverse strategies that have been implemented for developing transgenic mouse and rat lines and highlight recent advances that have led to dramatic improvements in achieving functional transgene expression of engineered opsins. Furthermore, we discuss considerations and caveats associated with implementing recently developed transgenic lines for optogenetics-based experimentation. Lastly, we propose strategies that can be implemented to develop and refine the next generation of genetically modified animals for behaviorally-focused optogenetics-based applications. |
| first_indexed | 2024-09-23T16:38:38Z |
| format | Article |
| id | mit-1721.1/102413 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:38:38Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1024132022-09-29T20:32:34Z Development of transgenic animals for optogenetic manipulation of mammalian nervous system function: Progress and prospects for behavioral neuroscience Feng, Guoping Ting, Jonathan Thomas Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Ting, Jonathan Thomas Feng, Guoping Here we review the rapidly growing toolbox of transgenic mice and rats that exhibit functional expression of engineered opsins for neuronal activation and silencing with light. Collectively, these transgenic animals are enabling neuroscientists to access and manipulate the many diverse cell types in the mammalian nervous system in order to probe synaptic and circuitry connectivity, function, and dysfunction. The availability of transgenic lines affords important advantages such as stable and heritable transgene expression patterns across experimental cohorts. As such, the use of transgenic lines precludes the need for other costly and labor-intensive procedures to achieve functional transgene expression in each individual experimental animal. This represents an important consideration when large cohorts of experimental animals are desirable as in many common behavioral assays. We describe the diverse strategies that have been implemented for developing transgenic mouse and rat lines and highlight recent advances that have led to dramatic improvements in achieving functional transgene expression of engineered opsins. Furthermore, we discuss considerations and caveats associated with implementing recently developed transgenic lines for optogenetics-based experimentation. Lastly, we propose strategies that can be implemented to develop and refine the next generation of genetically modified animals for behaviorally-focused optogenetics-based applications. Poitras Center for Affective Disorders Research National Institute of Mental Health (U.S.) (American Recovery and Reinvestment Act Grant RC1-MH088434) National Alliance for Research on Schizophrenia and Depression. The Brain and Behavior Research Foundation (Young Investigator Award) National Institutes of Health (U.S.) (Ruth L. Kirschstein National Research Service Award F32MH084460) 2016-05-05T00:16:36Z 2016-05-05T00:16:36Z 2013-03 2013-02 Article http://purl.org/eprint/type/JournalArticle 01664328 http://hdl.handle.net/1721.1/102413 Ting, Jonathan T., and Guoping Feng. “Development of Transgenic Animals for Optogenetic Manipulation of Mammalian Nervous System Function: Progress and Prospects for Behavioral Neuroscience.” Behavioural Brain Research 255 (October 2013): 3–18. https://orcid.org/0000-0002-8021-277X en_US http://dx.doi.org/10.1016/j.bbr.2013.02.037 Behavioural Brain Research Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Feng, Guoping Ting, Jonathan Thomas Development of transgenic animals for optogenetic manipulation of mammalian nervous system function: Progress and prospects for behavioral neuroscience |
| title | Development of transgenic animals for optogenetic manipulation of mammalian nervous system function: Progress and prospects for behavioral neuroscience |
| title_full | Development of transgenic animals for optogenetic manipulation of mammalian nervous system function: Progress and prospects for behavioral neuroscience |
| title_fullStr | Development of transgenic animals for optogenetic manipulation of mammalian nervous system function: Progress and prospects for behavioral neuroscience |
| title_full_unstemmed | Development of transgenic animals for optogenetic manipulation of mammalian nervous system function: Progress and prospects for behavioral neuroscience |
| title_short | Development of transgenic animals for optogenetic manipulation of mammalian nervous system function: Progress and prospects for behavioral neuroscience |
| title_sort | development of transgenic animals for optogenetic manipulation of mammalian nervous system function progress and prospects for behavioral neuroscience |
| url | http://hdl.handle.net/1721.1/102413 https://orcid.org/0000-0002-8021-277X |
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