No current evidence for widespread dosage compensation in S. cerevisiae
Previous studies of laboratory strains of budding yeast had shown that when gene copy number is altered experimentally, RNA levels generally scale accordingly. This is true when the copy number of individual genes or entire chromosomes is altered. In a recent study, Hose et al. (2015) reported that...
| Main Authors: | , , |
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| Format: | Article |
| Language: | en_US |
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eLife Sciences Publications, Ltd.
2016
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| Online Access: | http://hdl.handle.net/1721.1/102626 https://orcid.org/0000-0001-9837-0314 |
| _version_ | 1826210311941652480 |
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| author | Springer, Michael Torres, Eduardo M. Amon, Angelika B |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Springer, Michael Torres, Eduardo M. Amon, Angelika B |
| author_sort | Springer, Michael |
| collection | MIT |
| description | Previous studies of laboratory strains of budding yeast had shown that when gene copy number is altered experimentally, RNA levels generally scale accordingly. This is true when the copy number of individual genes or entire chromosomes is altered. In a recent study, Hose et al. (2015) reported that this tight correlation between gene copy number and RNA levels is not observed in recently isolated wild Saccharomyces cerevisiae variants. To understand the origins of this proposed difference in gene expression regulation between natural variants and laboratory strains of S. cerevisiae, we evaluated the karyotype and gene expression studies performed by Hose et al. on wild S. cerevisiae strains. In contrast to the results of Hose et al., our reexamination of their data revealed a tight correlation between gene copy number and gene expression. We conclude that widespread dosage compensation occurs neither in laboratory strains nor in natural variants of S. cerevisiae. |
| first_indexed | 2024-09-23T14:47:49Z |
| format | Article |
| id | mit-1721.1/102626 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:47:49Z |
| publishDate | 2016 |
| publisher | eLife Sciences Publications, Ltd. |
| record_format | dspace |
| spelling | mit-1721.1/1026262022-10-01T22:33:08Z No current evidence for widespread dosage compensation in S. cerevisiae Springer, Michael Torres, Eduardo M. Amon, Angelika B Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Amon, Angelika B. Previous studies of laboratory strains of budding yeast had shown that when gene copy number is altered experimentally, RNA levels generally scale accordingly. This is true when the copy number of individual genes or entire chromosomes is altered. In a recent study, Hose et al. (2015) reported that this tight correlation between gene copy number and RNA levels is not observed in recently isolated wild Saccharomyces cerevisiae variants. To understand the origins of this proposed difference in gene expression regulation between natural variants and laboratory strains of S. cerevisiae, we evaluated the karyotype and gene expression studies performed by Hose et al. on wild S. cerevisiae strains. In contrast to the results of Hose et al., our reexamination of their data revealed a tight correlation between gene copy number and gene expression. We conclude that widespread dosage compensation occurs neither in laboratory strains nor in natural variants of S. cerevisiae. National Institutes of Health (U.S.) (GM056800) 2016-05-23T17:07:12Z 2016-05-23T17:07:12Z 2016-03 2015-08 Article http://purl.org/eprint/type/JournalArticle 2050-084X http://hdl.handle.net/1721.1/102626 Torres, Eduardo M., Michael Springer, and Angelika Amon. "No current evidence for widespread dosage compensation in S. cerevisiae." eLife 2016;5:e10996 https://orcid.org/0000-0001-9837-0314 en_US http://dx.doi.org/10.7554/eLife.10996 eLife Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf eLife Sciences Publications, Ltd. eLife Sciences Publications, Ltd. |
| spellingShingle | Springer, Michael Torres, Eduardo M. Amon, Angelika B No current evidence for widespread dosage compensation in S. cerevisiae |
| title | No current evidence for widespread dosage compensation in S. cerevisiae |
| title_full | No current evidence for widespread dosage compensation in S. cerevisiae |
| title_fullStr | No current evidence for widespread dosage compensation in S. cerevisiae |
| title_full_unstemmed | No current evidence for widespread dosage compensation in S. cerevisiae |
| title_short | No current evidence for widespread dosage compensation in S. cerevisiae |
| title_sort | no current evidence for widespread dosage compensation in s cerevisiae |
| url | http://hdl.handle.net/1721.1/102626 https://orcid.org/0000-0001-9837-0314 |
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