Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
Polydimethylsiloxane (PDMS) is the prevailing building material to make microfluidic devices due to its ease of molding and bonding as well as its transparency. Due to the softness of the PDMS material, however, it is challenging to use PDMS for building nanochannels. The channels tend to collapse e...
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| Format: | Article |
| Language: | en_US |
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MyJoVE Corporation
2016
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| Online Access: | http://hdl.handle.net/1721.1/102694 https://orcid.org/0000-0001-7215-1439 |
| _version_ | 1826193830796328960 |
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| author | Wei, Xi Syed, Abeer Mao, Pan Han, Jongyoon Song, Yong-Ak |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Wei, Xi Syed, Abeer Mao, Pan Han, Jongyoon Song, Yong-Ak |
| author_sort | Wei, Xi |
| collection | MIT |
| description | Polydimethylsiloxane (PDMS) is the prevailing building material to make microfluidic devices due to its ease of molding and bonding as well as its transparency. Due to the softness of the PDMS material, however, it is challenging to use PDMS for building nanochannels. The channels tend to collapse easily during plasma bonding. In this paper, we present an evaporation-driven self-assembly method of silica colloidal nanoparticles to create nanofluidic junctions with sub-50 nm pores between two microchannels. The pore size as well as the surface charge of the nanofluidic junction is tunable simply by changing the colloidal silica bead size and surface functionalization outside of the assembled microfluidic device in a vial before the self-assembly process. Using the self-assembly of nanoparticles with a bead size of 300 nm, 500 nm, and 900 nm, it was possible to fabricate a porous membrane with a pore size of ~45 nm, ~75 nm and ~135 nm, respectively. Under electrical potential, this nanoporous membrane initiated ion concentration polarization (ICP) acting as a cation-selective membrane to concentrate DNA by ~1,700 times within 15 min. This non-lithographic nanofabrication process opens up a new opportunity to build a tunable nanofluidic junction for the study of nanoscale transport processes of ions and molecules inside a PDMS microfluidic chip. |
| first_indexed | 2024-09-23T09:46:04Z |
| format | Article |
| id | mit-1721.1/102694 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:46:04Z |
| publishDate | 2016 |
| publisher | MyJoVE Corporation |
| record_format | dspace |
| spelling | mit-1721.1/1026942022-09-30T16:42:33Z Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles Wei, Xi Syed, Abeer Mao, Pan Han, Jongyoon Song, Yong-Ak Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Han, Jongyoon Polydimethylsiloxane (PDMS) is the prevailing building material to make microfluidic devices due to its ease of molding and bonding as well as its transparency. Due to the softness of the PDMS material, however, it is challenging to use PDMS for building nanochannels. The channels tend to collapse easily during plasma bonding. In this paper, we present an evaporation-driven self-assembly method of silica colloidal nanoparticles to create nanofluidic junctions with sub-50 nm pores between two microchannels. The pore size as well as the surface charge of the nanofluidic junction is tunable simply by changing the colloidal silica bead size and surface functionalization outside of the assembled microfluidic device in a vial before the self-assembly process. Using the self-assembly of nanoparticles with a bead size of 300 nm, 500 nm, and 900 nm, it was possible to fabricate a porous membrane with a pore size of ~45 nm, ~75 nm and ~135 nm, respectively. Under electrical potential, this nanoporous membrane initiated ion concentration polarization (ICP) acting as a cation-selective membrane to concentrate DNA by ~1,700 times within 15 min. This non-lithographic nanofabrication process opens up a new opportunity to build a tunable nanofluidic junction for the study of nanoscale transport processes of ions and molecules inside a PDMS microfluidic chip. National Institutes of Health (U.S.) (NIH R21 EB008177-01A2) New York University Abu Dhabi (NYUAD) (Research Enhancement Fund 2013) 2016-05-26T19:42:58Z 2016-05-26T19:42:58Z 2016-03 Article http://purl.org/eprint/type/JournalArticle 1940-087X http://hdl.handle.net/1721.1/102694 Xi, Wei, Abeer Syed, Pan Mao, Jongyoon Han, and Yong-Ak Song. "Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles." JOVE: Engineering 109: e54145 (2016). https://orcid.org/0000-0001-7215-1439 en_US http://dx.doi.org/10.3791/54145 Journal of Visualized Experiments Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf MyJoVE Corporation MyJoVE Corporation |
| spellingShingle | Wei, Xi Syed, Abeer Mao, Pan Han, Jongyoon Song, Yong-Ak Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles |
| title | Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles |
| title_full | Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles |
| title_fullStr | Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles |
| title_full_unstemmed | Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles |
| title_short | Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles |
| title_sort | creating sub 50 nm nanofluidic junctions in pdms microfluidic chip via self assembly process of colloidal particles |
| url | http://hdl.handle.net/1721.1/102694 https://orcid.org/0000-0001-7215-1439 |
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