Mathematical analysis of oxygen transfer through polydimethylsiloxane membrane between double layers of cell culture channel and gas chamber in microfluidic oxygenator
For successful cell culture in microfluidic devices, precise control of the microenvironment, including gas transfer between the cells and the surrounding medium, is exceptionally important. The work is motivated by a polydimethylsiloxane (PDMS) microfluidic oxygenator chip for mammalian cell cultur...
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| Format: | Article |
| Language: | English |
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Springer Berlin Heidelberg
2016
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| Online Access: | http://hdl.handle.net/1721.1/103106 https://orcid.org/0000-0001-6649-9463 https://orcid.org/0000-0003-3155-6223 |
| _version_ | 1826217439580389376 |
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| author | Kim, Min-Cheol Lam, Raymond H. W. Thorsen, Todd Asada, Haruhiko Harry |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Kim, Min-Cheol Lam, Raymond H. W. Thorsen, Todd Asada, Haruhiko Harry |
| author_sort | Kim, Min-Cheol |
| collection | MIT |
| description | For successful cell culture in microfluidic devices, precise control of the microenvironment, including gas transfer between the cells and the surrounding medium, is exceptionally important. The work is motivated by a polydimethylsiloxane (PDMS) microfluidic oxygenator chip for mammalian cell culture suggesting that the speed of the oxygen transfer may vary depending on the thickness of a PDMS membrane or the height of a fluid channel. In this paper, a model is presented to describe the oxygen transfer dynamics in the PDMS microfluidic oxygenator chip for mammalian cell culture. Theoretical studies were carried out to evaluate the oxygen profile within the multilayer device, consisting of a gas reservoir, a PDMS membrane, a fluid channel containing growth media, and a cell culture layer. The corresponding semi-analytical solution was derived to evaluate dissolved oxygen concentration within the heterogeneous materials, and was found to be in good agreement with the numerical solution. In addition, a separate analytical solution was obtained to investigate the oxygen pressure drop (OPD) along the cell layer due to oxygen uptake of cells, with experimental validation of the OPD model carried out using human umbilical vein endothelial cells cultured in a PDMS microfluidic oxygenator. Within the theoretical framework, the effects of several microfluidic oxygenator design parameters were studied, including cell type and critical device dimensions. |
| first_indexed | 2024-09-23T17:04:00Z |
| format | Article |
| id | mit-1721.1/103106 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T17:04:00Z |
| publishDate | 2016 |
| publisher | Springer Berlin Heidelberg |
| record_format | dspace |
| spelling | mit-1721.1/1031062022-10-03T10:09:32Z Mathematical analysis of oxygen transfer through polydimethylsiloxane membrane between double layers of cell culture channel and gas chamber in microfluidic oxygenator Kim, Min-Cheol Lam, Raymond H. W. Thorsen, Todd Asada, Haruhiko Harry Massachusetts Institute of Technology. Department of Mechanical Engineering Kim, Min-Cheol Lam, Raymond H. W. Thorsen, Todd Asada, Haruhiko Harry For successful cell culture in microfluidic devices, precise control of the microenvironment, including gas transfer between the cells and the surrounding medium, is exceptionally important. The work is motivated by a polydimethylsiloxane (PDMS) microfluidic oxygenator chip for mammalian cell culture suggesting that the speed of the oxygen transfer may vary depending on the thickness of a PDMS membrane or the height of a fluid channel. In this paper, a model is presented to describe the oxygen transfer dynamics in the PDMS microfluidic oxygenator chip for mammalian cell culture. Theoretical studies were carried out to evaluate the oxygen profile within the multilayer device, consisting of a gas reservoir, a PDMS membrane, a fluid channel containing growth media, and a cell culture layer. The corresponding semi-analytical solution was derived to evaluate dissolved oxygen concentration within the heterogeneous materials, and was found to be in good agreement with the numerical solution. In addition, a separate analytical solution was obtained to investigate the oxygen pressure drop (OPD) along the cell layer due to oxygen uptake of cells, with experimental validation of the OPD model carried out using human umbilical vein endothelial cells cultured in a PDMS microfluidic oxygenator. Within the theoretical framework, the effects of several microfluidic oxygenator design parameters were studied, including cell type and critical device dimensions. Singapore-MIT Alliance for Research and Technology (SMART) Croucher Foundation Research Grants Council (Hong Kong, China) (Early Career Scheme, (Project# RGC124212)) National Science Foundation (U.S.) (Grant No. EFRI-0735997) National Science Foundation (U.S.) (Grant No. STC- 0902396) 2016-06-14T16:11:27Z 2016-06-14T16:11:27Z 2013-02 2012-09 2016-05-23T12:11:47Z Article http://purl.org/eprint/type/JournalArticle 1613-4982 1613-4990 http://hdl.handle.net/1721.1/103106 Kim, Min-Cheol, Raymond H. W. Lam, Todd Thorsen, and H. Harry Asada. “Mathematical Analysis of Oxygen Transfer through Polydimethylsiloxane Membrane Between Double Layers of Cell Culture Channel and Gas Chamber in Microfluidic Oxygenator.” Microfluid Nanofluid 15, no. 3 (February 1, 2013): 285–296. https://orcid.org/0000-0001-6649-9463 https://orcid.org/0000-0003-3155-6223 en http://dx.doi.org/10.1007/s10404-013-1142-8 Microfluidics and Nanofluidics Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ Springer-Verlag Berlin Heidelberg application/pdf Springer Berlin Heidelberg Springer Berlin Heidelberg |
| spellingShingle | Kim, Min-Cheol Lam, Raymond H. W. Thorsen, Todd Asada, Haruhiko Harry Mathematical analysis of oxygen transfer through polydimethylsiloxane membrane between double layers of cell culture channel and gas chamber in microfluidic oxygenator |
| title | Mathematical analysis of oxygen transfer through polydimethylsiloxane membrane between double layers of cell culture channel and gas chamber in microfluidic oxygenator |
| title_full | Mathematical analysis of oxygen transfer through polydimethylsiloxane membrane between double layers of cell culture channel and gas chamber in microfluidic oxygenator |
| title_fullStr | Mathematical analysis of oxygen transfer through polydimethylsiloxane membrane between double layers of cell culture channel and gas chamber in microfluidic oxygenator |
| title_full_unstemmed | Mathematical analysis of oxygen transfer through polydimethylsiloxane membrane between double layers of cell culture channel and gas chamber in microfluidic oxygenator |
| title_short | Mathematical analysis of oxygen transfer through polydimethylsiloxane membrane between double layers of cell culture channel and gas chamber in microfluidic oxygenator |
| title_sort | mathematical analysis of oxygen transfer through polydimethylsiloxane membrane between double layers of cell culture channel and gas chamber in microfluidic oxygenator |
| url | http://hdl.handle.net/1721.1/103106 https://orcid.org/0000-0001-6649-9463 https://orcid.org/0000-0003-3155-6223 |
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