Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis
Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended microchannel resonator to monitor the volume and density of single cells in suspensi...
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| Format: | Article |
| Language: | en_US |
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Rockefeller University Press
2016
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| Online Access: | http://hdl.handle.net/1721.1/103125 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-4165-7538 |
| _version_ | 1826192113078894592 |
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| author | Son, Sungmin Kang, Joon Ho Oh, Seungeun Kirschner, Marc W. Mitchison, T.J. Manalis, Scott R |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Son, Sungmin Kang, Joon Ho Oh, Seungeun Kirschner, Marc W. Mitchison, T.J. Manalis, Scott R |
| author_sort | Son, Sungmin |
| collection | MIT |
| description | Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended microchannel resonator to monitor the volume and density of single cells in suspension with a precision of 1% and 0.03%, respectively. We find that for transformed murine lymphocytic leukemia and mouse pro–B cell lymphoid cell lines, mitotic cells reversibly increase their volume by more than 10% and decrease their density by 0.4% over a 20-min period. This response is correlated with the mitotic cell cycle but is not coupled to nuclear osmolytes released by nuclear envelope breakdown, chromatin condensation, or cytokinesis and does not result from endocytosis of the surrounding fluid. Inhibiting Na-H exchange eliminates the response. Although mitotic rounding of adherent cells is necessary for proper cell division, our observations that suspended cells undergo reversible swelling during mitosis suggest that regulation of intracellular water may be a more general component of mitosis than previously appreciated. |
| first_indexed | 2024-09-23T09:06:27Z |
| format | Article |
| id | mit-1721.1/103125 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:06:27Z |
| publishDate | 2016 |
| publisher | Rockefeller University Press |
| record_format | dspace |
| spelling | mit-1721.1/1031252022-09-26T10:32:09Z Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis Son, Sungmin Kang, Joon Ho Oh, Seungeun Kirschner, Marc W. Mitchison, T.J. Manalis, Scott R Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Physics Koch Institute for Integrative Cancer Research at MIT Son, Sungmin Kang, Joon Ho Manalis, Scott R. Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended microchannel resonator to monitor the volume and density of single cells in suspension with a precision of 1% and 0.03%, respectively. We find that for transformed murine lymphocytic leukemia and mouse pro–B cell lymphoid cell lines, mitotic cells reversibly increase their volume by more than 10% and decrease their density by 0.4% over a 20-min period. This response is correlated with the mitotic cell cycle but is not coupled to nuclear osmolytes released by nuclear envelope breakdown, chromatin condensation, or cytokinesis and does not result from endocytosis of the surrounding fluid. Inhibiting Na-H exchange eliminates the response. Although mitotic rounding of adherent cells is necessary for proper cell division, our observations that suspended cells undergo reversible swelling during mitosis suggest that regulation of intracellular water may be a more general component of mitosis than previously appreciated. National Cancer Institute (U.S.). Physical Sciences-Oncology Center (MIT, (U54CA143874)) National Cancer Institute (U.S.) (Koch Institute Support (core) grant P30-CA14051) Samsung Scholarship Foundation 2016-06-16T19:41:19Z 2016-06-16T19:41:19Z 2015-11 2015-05 Article http://purl.org/eprint/type/JournalArticle 0021-9525 1540-8140 http://hdl.handle.net/1721.1/103125 Son, Sungmin, Joon Ho Kang, Seungeun Oh, Marc W. Kirschner, T.J. Mitchison, and Scott Manalis. “Resonant Microchannel Volume and Mass Measurements Show That Suspended Cells Swell During Mitosis.” Journal of Cell Biology 211, no. 4 (November 23, 2015): 757–763. https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-4165-7538 en_US http://dx.doi.org/10.1083/jcb.201505058 Journal of Cell Biology Creative Commons Attribution http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Rockefeller University Press Rockefeller University Press |
| spellingShingle | Son, Sungmin Kang, Joon Ho Oh, Seungeun Kirschner, Marc W. Mitchison, T.J. Manalis, Scott R Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis |
| title | Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis |
| title_full | Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis |
| title_fullStr | Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis |
| title_full_unstemmed | Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis |
| title_short | Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis |
| title_sort | resonant microchannel volume and mass measurements show that suspended cells swell during mitosis |
| url | http://hdl.handle.net/1721.1/103125 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-4165-7538 |
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