In silico tools for the development of biotherapeutics
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, February 2016.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2016
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| Online Access: | http://hdl.handle.net/1721.1/103276 |
| _version_ | 1826215198675959808 |
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| author | Lauer, Timothy Michael |
| author2 | Bernhardt L. Trout. |
| author_facet | Bernhardt L. Trout. Lauer, Timothy Michael |
| author_sort | Lauer, Timothy Michael |
| collection | MIT |
| description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, February 2016. |
| first_indexed | 2024-09-23T16:18:50Z |
| format | Thesis |
| id | mit-1721.1/103276 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T16:18:50Z |
| publishDate | 2016 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1032762019-04-12T16:27:24Z In silico tools for the development of biotherapeutics Lauer, Timothy Michael Bernhardt L. Trout. Massachusetts Institute of Technology. Department of Chemical Engineering. Massachusetts Institute of Technology. Department of Chemical Engineering. Chemical Engineering. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, February 2016. Cataloged from PDF version of thesis. Includes bibliographical references (pages 122-137). The production of a new drug is an inherently risky process. While there are many causes for failure, a number of these are due to the many potential degradation pathways a protein can undergo. Often these reactions are slow and negligible. However, there are times where any one of these reactions can be significant enough to delay or prevent a drug's development. Testing for all of these degradation routes can be difficult in the early stages of drug development due to small amount of available protein and the long times needed to sample these reactions. In order to reduce the risk to the drug development process, in silico methods have been developed to predict the likelihood of these reactions, without the need for any material. This work focuses on two degradation routes; the aggregation pathway and the acidcatalyzed, non-enzymatic hydrolysis of peptide bonds. Aggregation of antibodies can be a limiting factor for liquid formulations; however, two major factors control this reaction: the surface hydrophobicity and the protein charge. These two factors were combined into a new tool, called the Developability Index, to predict protein aggregation rates. This tool was successfully applied to both antibody and individual antibody domains. The non-enzymatic, acid-catalyzed hydrolysis of an amide bond following an aspartic or glutamic acid residue is controlled by a wider range of factors. These include: the secondary structure, the surface exposure of the amide bond, relative orientation of the sidechain, and the availability of the sidechain. These four factors impact the first two steps of the hydrolysis mechanism: the addition of the proton to the peptide bond and the addition of the sidechain to the peptide backbone. The secondary structure and surface exposure of the peptide bond impact the ability of the proton to add to the peptide bond, and thus start the reaction, while the orientation and availability of the sidechain impact the ability of the sidechain to cyclize and form a ring. These factors can be combined to produce a method to predict the reactivity of peptide bonds with high accuracy. by Timothy Michael Lauer. Ph. D. 2016-06-22T17:55:13Z 2016-06-22T17:55:13Z 2015 2016 Thesis http://hdl.handle.net/1721.1/103276 951811731 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 154 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemical Engineering. Lauer, Timothy Michael In silico tools for the development of biotherapeutics |
| title | In silico tools for the development of biotherapeutics |
| title_full | In silico tools for the development of biotherapeutics |
| title_fullStr | In silico tools for the development of biotherapeutics |
| title_full_unstemmed | In silico tools for the development of biotherapeutics |
| title_short | In silico tools for the development of biotherapeutics |
| title_sort | in silico tools for the development of biotherapeutics |
| topic | Chemical Engineering. |
| url | http://hdl.handle.net/1721.1/103276 |
| work_keys_str_mv | AT lauertimothymichael insilicotoolsforthedevelopmentofbiotherapeutics |