Microbiota of the Major South Atlantic Reef Building Coral Mussismilia
The Brazilian endemic scleractinian corals, genus Mussismilia, are among the main reef builders of the South Atlantic and are threatened by accelerating rates of disease. To better understand how holobiont microbial populations interact with corals during health and disease and to evaluate whether s...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer US
2016
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| Online Access: | http://hdl.handle.net/1721.1/103330 https://orcid.org/0000-0001-8667-8186 |
| _version_ | 1811075451411496960 |
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| author | Fernando, Samodha C. Wang, Jia Sparling, Kimberly M. Garcia, Gizele D. Francini-Filho, Ronaldo B. de Moura, Rodrigo L. Paranhos, Rodolfo Thompson, Fabiano L. Thompson, Janelle Renee |
| author2 | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Fernando, Samodha C. Wang, Jia Sparling, Kimberly M. Garcia, Gizele D. Francini-Filho, Ronaldo B. de Moura, Rodrigo L. Paranhos, Rodolfo Thompson, Fabiano L. Thompson, Janelle Renee |
| author_sort | Fernando, Samodha C. |
| collection | MIT |
| description | The Brazilian endemic scleractinian corals, genus Mussismilia, are among the main reef builders of the South Atlantic and are threatened by accelerating rates of disease. To better understand how holobiont microbial populations interact with corals during health and disease and to evaluate whether selective pressures in the holobiont or neutral assembly shape microbial composition, we have examined the microbiota structure of Mussismilia corals according to coral lineage, environment, and disease/health status. Microbiota of three Mussismilia species (Mussismilia harttii, Mussismilia hispida, and Mussismilia braziliensis) was compared using 16S rRNA pyrosequencing and clone library analysis of coral fragments. Analysis of biological triplicates per Mussismilia species and reef site allowed assessment of variability among Mussismilia species and between sites for M. braziliensis. From 173,487 V6 sequences, 6,733 coral- and 1,052 water-associated operational taxonomic units (OTUs) were observed. M. braziliensis microbiota was more similar across reefs than to other Mussismilia species microbiota from the same reef. Highly prevalent OTUs were more significantly structured by coral lineage and were enriched in Alpha- and Gammaproteobacteria. Bacterial OTUs from healthy corals were recovered from a M. braziliensis skeleton sample at twice the frequency of recovery from water or a diseased coral suggesting the skeleton is a significant habitat for microbial populations in the holobiont. Diseased corals were enriched with pathogens and opportunists (Vibrios, Bacteroidetes, Thalassomonas, and SRB). Our study examines for the first time intra- and inter-specific variability of microbiota across the genus Mussismilia. Changes in microbiota may be useful indicators of coral health and thus be a valuable tool for coral reef management and conservation. |
| first_indexed | 2024-09-23T10:06:11Z |
| format | Article |
| id | mit-1721.1/103330 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:06:11Z |
| publishDate | 2016 |
| publisher | Springer US |
| record_format | dspace |
| spelling | mit-1721.1/1033302022-09-30T18:57:02Z Microbiota of the Major South Atlantic Reef Building Coral Mussismilia Fernando, Samodha C. Wang, Jia Sparling, Kimberly M. Garcia, Gizele D. Francini-Filho, Ronaldo B. de Moura, Rodrigo L. Paranhos, Rodolfo Thompson, Fabiano L. Thompson, Janelle Renee Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Fernando, Samodha C. Wang, Jia Sparling, Kimberly M. Thompson, Janelle Renee The Brazilian endemic scleractinian corals, genus Mussismilia, are among the main reef builders of the South Atlantic and are threatened by accelerating rates of disease. To better understand how holobiont microbial populations interact with corals during health and disease and to evaluate whether selective pressures in the holobiont or neutral assembly shape microbial composition, we have examined the microbiota structure of Mussismilia corals according to coral lineage, environment, and disease/health status. Microbiota of three Mussismilia species (Mussismilia harttii, Mussismilia hispida, and Mussismilia braziliensis) was compared using 16S rRNA pyrosequencing and clone library analysis of coral fragments. Analysis of biological triplicates per Mussismilia species and reef site allowed assessment of variability among Mussismilia species and between sites for M. braziliensis. From 173,487 V6 sequences, 6,733 coral- and 1,052 water-associated operational taxonomic units (OTUs) were observed. M. braziliensis microbiota was more similar across reefs than to other Mussismilia species microbiota from the same reef. Highly prevalent OTUs were more significantly structured by coral lineage and were enriched in Alpha- and Gammaproteobacteria. Bacterial OTUs from healthy corals were recovered from a M. braziliensis skeleton sample at twice the frequency of recovery from water or a diseased coral suggesting the skeleton is a significant habitat for microbial populations in the holobiont. Diseased corals were enriched with pathogens and opportunists (Vibrios, Bacteroidetes, Thalassomonas, and SRB). Our study examines for the first time intra- and inter-specific variability of microbiota across the genus Mussismilia. Changes in microbiota may be useful indicators of coral health and thus be a valuable tool for coral reef management and conservation. MIT International Science and Technology Initiatives (MIT-Brazil seed Grants Program) Conselho Nacional de Pesquisas (Brazil) (Grant) Carlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro Brazil. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (grant) United States. Embassy (grant) Massachusetts Institute of Technology. Center for Environmental Health Sciences 2016-06-24T18:04:34Z 2016-06-24T18:04:34Z 2014-09 2014-03 2016-05-23T12:14:04Z Article http://purl.org/eprint/type/JournalArticle 0095-3628 1432-184X http://hdl.handle.net/1721.1/103330 Fernando, Samodha C., Jia Wang, Kimberly Sparling, Gizele D. Garcia, Ronaldo B. Francini-Filho, Rodrigo L. de Moura, Rodolfo Paranhos, Fabiano L. Thompson, and Janelle R. Thompson. “Microbiota of the Major South Atlantic Reef Building Coral Mussismilia.” Microbial Ecology 69, no. 2 (September 12, 2014): 267–280. https://orcid.org/0000-0001-8667-8186 en http://dx.doi.org/10.1007/s00248-014-0474-6 Microbial Ecology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ Springer Science+Business Media New York application/pdf Springer US Springer US |
| spellingShingle | Fernando, Samodha C. Wang, Jia Sparling, Kimberly M. Garcia, Gizele D. Francini-Filho, Ronaldo B. de Moura, Rodrigo L. Paranhos, Rodolfo Thompson, Fabiano L. Thompson, Janelle Renee Microbiota of the Major South Atlantic Reef Building Coral Mussismilia |
| title | Microbiota of the Major South Atlantic Reef Building Coral Mussismilia |
| title_full | Microbiota of the Major South Atlantic Reef Building Coral Mussismilia |
| title_fullStr | Microbiota of the Major South Atlantic Reef Building Coral Mussismilia |
| title_full_unstemmed | Microbiota of the Major South Atlantic Reef Building Coral Mussismilia |
| title_short | Microbiota of the Major South Atlantic Reef Building Coral Mussismilia |
| title_sort | microbiota of the major south atlantic reef building coral mussismilia |
| url | http://hdl.handle.net/1721.1/103330 https://orcid.org/0000-0001-8667-8186 |
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