Assessment of colorimetric amplification methods in a paper-based immunoassay for diagnosis of malaria
Colorimetric detection methods that produce results readable by eye are important for diagnostic tests in resource-limited settings. In this work, we have compared three main types of colorimetric methods – enzymatic reactions, silver deposition catalyzed by gold nanoparticles, and polymerization-ba...
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| Format: | Article |
| Language: | en_US |
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Royal Society of Chemistry
2016
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| Online Access: | http://hdl.handle.net/1721.1/103541 https://orcid.org/0000-0002-7096-138X https://orcid.org/0000-0001-5422-4366 |
| _version_ | 1826207619042246656 |
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| author | Lathwal, Shefali Sikes, Hadley |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Lathwal, Shefali Sikes, Hadley |
| author_sort | Lathwal, Shefali |
| collection | MIT |
| description | Colorimetric detection methods that produce results readable by eye are important for diagnostic tests in resource-limited settings. In this work, we have compared three main types of colorimetric methods – enzymatic reactions, silver deposition catalyzed by gold nanoparticles, and polymerization-based amplification – in a paper-based immunoassay for detection of Plasmodium falciparum histidine-rich protein 2, a biomarker of malarial infection. We kept the binding events in the immunoassay constant in order to isolate the effect of the detection method on the outcome of the test. We have highlighted that the optimal readout time in a test can vary significantly – ranging from immediately after addition of a visualization agent to 25 minutes after addition of a visualization agent – depending on the colorimetric method being used, and accurate time keeping is essential to prevent false positives in methods where substantial color develops over time in negative tests. We have also shown that the choice of a colorimetric method impacts the calculated limit-of-detection, the ease of visual perception of the readout, and the total cost of the assay, and therefore directly impacts the feasibility and the ease-of-use of a test in field settings. |
| first_indexed | 2024-09-23T13:52:23Z |
| format | Article |
| id | mit-1721.1/103541 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:52:23Z |
| publishDate | 2016 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/1035412022-09-28T16:46:17Z Assessment of colorimetric amplification methods in a paper-based immunoassay for diagnosis of malaria Lathwal, Shefali Sikes, Hadley Massachusetts Institute of Technology. Department of Chemical Engineering Lathwal, Shefali Sikes, Hadley Colorimetric detection methods that produce results readable by eye are important for diagnostic tests in resource-limited settings. In this work, we have compared three main types of colorimetric methods – enzymatic reactions, silver deposition catalyzed by gold nanoparticles, and polymerization-based amplification – in a paper-based immunoassay for detection of Plasmodium falciparum histidine-rich protein 2, a biomarker of malarial infection. We kept the binding events in the immunoassay constant in order to isolate the effect of the detection method on the outcome of the test. We have highlighted that the optimal readout time in a test can vary significantly – ranging from immediately after addition of a visualization agent to 25 minutes after addition of a visualization agent – depending on the colorimetric method being used, and accurate time keeping is essential to prevent false positives in methods where substantial color develops over time in negative tests. We have also shown that the choice of a colorimetric method impacts the calculated limit-of-detection, the ease of visual perception of the readout, and the total cost of the assay, and therefore directly impacts the feasibility and the ease-of-use of a test in field settings. Massachusetts Institute of Technology (Jonathan Whitney Fund) Massachusetts Institute of Technology (Haas Family Fellowship in Chemical Engineering) Massachusetts Institute of Technology (Charles E. Reed Faculty Initiative Fund) 2016-07-07T19:37:20Z 2016-07-07T19:37:20Z 2016-04 2016-01 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/103541 Lathwal, Shefali, and Hadley D. Sikes. “Assessment of Colorimetric Amplification Methods in a Paper-Based Immunoassay for Diagnosis of Malaria.” Lab on a Chip 16, no. 8 (2016): 1374–1382. https://orcid.org/0000-0002-7096-138X https://orcid.org/0000-0001-5422-4366 en_US http://dx.doi.org/10.1039/c6lc00058d Lab on a Chip Creative Commons Attribution-NonCommercial 3.0 Unported licence http://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry Royal Society of Chemistry |
| spellingShingle | Lathwal, Shefali Sikes, Hadley Assessment of colorimetric amplification methods in a paper-based immunoassay for diagnosis of malaria |
| title | Assessment of colorimetric amplification methods in a paper-based immunoassay for diagnosis of malaria |
| title_full | Assessment of colorimetric amplification methods in a paper-based immunoassay for diagnosis of malaria |
| title_fullStr | Assessment of colorimetric amplification methods in a paper-based immunoassay for diagnosis of malaria |
| title_full_unstemmed | Assessment of colorimetric amplification methods in a paper-based immunoassay for diagnosis of malaria |
| title_short | Assessment of colorimetric amplification methods in a paper-based immunoassay for diagnosis of malaria |
| title_sort | assessment of colorimetric amplification methods in a paper based immunoassay for diagnosis of malaria |
| url | http://hdl.handle.net/1721.1/103541 https://orcid.org/0000-0002-7096-138X https://orcid.org/0000-0001-5422-4366 |
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