Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique c...
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| Format: | Article |
| Language: | en_US |
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Springer Nature
2016
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| Online Access: | http://hdl.handle.net/1721.1/103551 https://orcid.org/0000-0003-1812-6421 https://orcid.org/0000-0002-2079-0543 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0002-3254-3224 |
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| author | Prevedel, Robert Yoon, Young-Gyu Hoffmann, Maximilian Pak, Nikita Wetzstein, Gordon Kato, Saul Schrödel, Tina Raskar, Ramesh Zimmer, Manuel Boyden, Edward Stuart Vaziri, Alipasha |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Prevedel, Robert Yoon, Young-Gyu Hoffmann, Maximilian Pak, Nikita Wetzstein, Gordon Kato, Saul Schrödel, Tina Raskar, Ramesh Zimmer, Manuel Boyden, Edward Stuart Vaziri, Alipasha |
| author_sort | Prevedel, Robert |
| collection | MIT |
| description | High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ~700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging. |
| first_indexed | 2024-09-23T16:08:44Z |
| format | Article |
| id | mit-1721.1/103551 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:08:44Z |
| publishDate | 2016 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | mit-1721.1/1035512022-09-29T18:34:14Z Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy Prevedel, Robert Yoon, Young-Gyu Hoffmann, Maximilian Pak, Nikita Wetzstein, Gordon Kato, Saul Schrödel, Tina Raskar, Ramesh Zimmer, Manuel Boyden, Edward Stuart Vaziri, Alipasha Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Media Laboratory McGovern Institute for Brain Research at MIT Yoon, Young-Gyu Pak, Nikita Wetzstein, Gordon Raskar, Ramesh Boyden, Edward Stuart High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ~700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging. European Commission (Marie Curie, FP7-PEOPLE-2011-IIF) National Institutes of Health (U.S.) (NIH grant 1R01EY023173) National Science Foundation (U.S.) (NSF CBET 1053233) National Science Foundation (U.S.) (NSF CBET 1344219) National Institutes of Health (U.S.) (NIH 1DP1NS087724) Vienna Science and Technology Fund ((WWTF) project VRG10-11) Seventh Framework Programme (European Commission) (ERC no. 281869) MIT Media Lab Consortium Institution of Engineering and Technology (IET) (Harvey Prize) Human Frontier Science Program (Strasbourg, France) (Project RGP0041/2012) 2016-07-08T16:56:10Z 2016-07-08T16:56:10Z 2014-05 2014-01 Article http://purl.org/eprint/type/JournalArticle 1548-7091 1548-7105 http://hdl.handle.net/1721.1/103551 Prevedel, Robert, Young-Gyu Yoon, Maximilian Hoffmann, Nikita Pak, Gordon Wetzstein, Saul Kato, Tina Schrödel, Ramesh Raskar, Manuel Zimmer, Edward S. Boyden, and Alipasha Vaziri. “Simultaneous Whole-Animal 3D Imaging of Neuronal Activity Using Light-Field Microscopy.” Nature Methods 11, no. 7 (May 18, 2014): 727–730. https://orcid.org/0000-0003-1812-6421 https://orcid.org/0000-0002-2079-0543 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0002-3254-3224 en_US http://dx.doi.org/10.1038/nmeth.2964 Nature Methods Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer Nature PMC |
| spellingShingle | Prevedel, Robert Yoon, Young-Gyu Hoffmann, Maximilian Pak, Nikita Wetzstein, Gordon Kato, Saul Schrödel, Tina Raskar, Ramesh Zimmer, Manuel Boyden, Edward Stuart Vaziri, Alipasha Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy |
| title | Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy |
| title_full | Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy |
| title_fullStr | Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy |
| title_full_unstemmed | Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy |
| title_short | Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy |
| title_sort | simultaneous whole animal 3d imaging of neuronal activity using light field microscopy |
| url | http://hdl.handle.net/1721.1/103551 https://orcid.org/0000-0003-1812-6421 https://orcid.org/0000-0002-2079-0543 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0002-3254-3224 |
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