Expansion microscopy
Available in PMC 2015 July 30.
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Association for the Advancement of Science (AAAS)
2016
|
| Online Access: | http://hdl.handle.net/1721.1/103552 https://orcid.org/0000-0003-0254-4741 https://orcid.org/0000-0002-0419-3351 |
| _version_ | 1826203393131020288 |
|---|---|
| author | Chen, Fei Tillberg, Paul W. Boyden, Edward |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Chen, Fei Tillberg, Paul W. Boyden, Edward |
| author_sort | Chen, Fei |
| collection | MIT |
| description | Available in PMC 2015 July 30. |
| first_indexed | 2024-09-23T12:36:11Z |
| format | Article |
| id | mit-1721.1/103552 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:36:11Z |
| publishDate | 2016 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/1035522022-09-28T08:57:56Z Expansion microscopy Chen, Fei Tillberg, Paul W. Boyden, Edward Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Media Laboratory McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Center for Neurobiological Engineering Chen, Fei Tillberg, Paul W. Boyden, Edward Stuart Available in PMC 2015 July 30. In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~107 cubic micrometers of the mouse hippocampus with a conventional confocal microscope. National Institutes of Health (U.S.) (NIH Director’s Pioneer Award 1DP1NS087724) National Institutes of Health (U.S.) (NIH Transformative Research Award 1R01MH103910-01) New York Stem Cell Foundation (Robertson Investigator Award) National Science Foundation (U.S.). Center for Brains, Minds and Machines (CBMM) (NSF CCF-1231216) National Science Foundation (U.S.) (NSF CAREER Award CBET 1053233) 2016-07-08T17:24:37Z 2016-07-08T17:24:37Z 2015-01 Article http://purl.org/eprint/type/JournalArticle 0036-8075 1095-9203 http://hdl.handle.net/1721.1/103552 Chen, Fei, Paul W. Tillberg, and Edward S. Boyden. “Expansion Microscopy.” Science 347, no. 6221 (January 15, 2015): 543–548. https://orcid.org/0000-0003-0254-4741 https://orcid.org/0000-0002-0419-3351 en_US http://dx.doi.org/10.1126/science.1260088 Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Association for the Advancement of Science (AAAS) PMC |
| spellingShingle | Chen, Fei Tillberg, Paul W. Boyden, Edward Expansion microscopy |
| title | Expansion microscopy |
| title_full | Expansion microscopy |
| title_fullStr | Expansion microscopy |
| title_full_unstemmed | Expansion microscopy |
| title_short | Expansion microscopy |
| title_sort | expansion microscopy |
| url | http://hdl.handle.net/1721.1/103552 https://orcid.org/0000-0003-0254-4741 https://orcid.org/0000-0002-0419-3351 |
| work_keys_str_mv | AT chenfei expansionmicroscopy AT tillbergpaulw expansionmicroscopy AT boydenedward expansionmicroscopy |