Optogenetic Inactivation Modifies Monkey Visuomotor Behavior
A critical technique for understanding how neuronal activity contributes to behavior is determining whether perturbing it changes behavior. The advent of optogenetic techniques allows the immediately reversible alteration of neuronal activity in contrast to chemical approaches lasting minutes to hou...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2016
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| Online Access: | http://hdl.handle.net/1721.1/103646 https://orcid.org/0000-0002-0419-3351 |
| _version_ | 1811097421836451840 |
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| author | Cavanaugh, James Monosov, Ilya E. McAlonan, Kerry Berman, Rebecca Smith, Mitchell K. Cao, Vania Wang, Kuan H. Boyden, Edward Stuart Wurtz, Robert H. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Cavanaugh, James Monosov, Ilya E. McAlonan, Kerry Berman, Rebecca Smith, Mitchell K. Cao, Vania Wang, Kuan H. Boyden, Edward Stuart Wurtz, Robert H. |
| author_sort | Cavanaugh, James |
| collection | MIT |
| description | A critical technique for understanding how neuronal activity contributes to behavior is determining whether perturbing it changes behavior. The advent of optogenetic techniques allows the immediately reversible alteration of neuronal activity in contrast to chemical approaches lasting minutes to hours. Modification of behavior using optogenetics has had substantial success in rodents but has not been as successful in monkeys. Here, we show how optogenetic inactivation of superior colliculus neurons in awake monkeys leads to clear and repeatable behavioral deficits in the metrics of saccadic eye movements. We used our observations to evaluate principles governing the use of optogenetic techniques in the study of the neuronal bases of behavior in monkeys, particularly how experimental design must address relevant parameters, such as the application of light to subcortical structures, the spread of viral injections, and the extent of neuronal inactivation with light. |
| first_indexed | 2024-09-23T16:59:16Z |
| format | Article |
| id | mit-1721.1/103646 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:59:16Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1036462022-09-29T22:55:37Z Optogenetic Inactivation Modifies Monkey Visuomotor Behavior Cavanaugh, James Monosov, Ilya E. McAlonan, Kerry Berman, Rebecca Smith, Mitchell K. Cao, Vania Wang, Kuan H. Boyden, Edward Stuart Wurtz, Robert H. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Boyden, Edward Stuart A critical technique for understanding how neuronal activity contributes to behavior is determining whether perturbing it changes behavior. The advent of optogenetic techniques allows the immediately reversible alteration of neuronal activity in contrast to chemical approaches lasting minutes to hours. Modification of behavior using optogenetics has had substantial success in rodents but has not been as successful in monkeys. Here, we show how optogenetic inactivation of superior colliculus neurons in awake monkeys leads to clear and repeatable behavioral deficits in the metrics of saccadic eye movements. We used our observations to evaluate principles governing the use of optogenetic techniques in the study of the neuronal bases of behavior in monkeys, particularly how experimental design must address relevant parameters, such as the application of light to subcortical structures, the spread of viral injections, and the extent of neuronal inactivation with light. 2016-07-18T17:26:32Z 2016-07-18T17:26:32Z 2012-12 2012-10 Article http://purl.org/eprint/type/JournalArticle 08966273 http://hdl.handle.net/1721.1/103646 Cavanaugh, James, Ilya E. Monosov, Kerry McAlonan, Rebecca Berman, Mitchell K. Smith, Vania Cao, Kuan H. Wang, Edward S. Boyden, and Robert H. Wurtz. “Optogenetic Inactivation Modifies Monkey Visuomotor Behavior.” Neuron 76, no. 5 (December 2012): 901–907. https://orcid.org/0000-0002-0419-3351 en_US http://dx.doi.org/10.1016/j.neuron.2012.10.016 Neuron Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Cavanaugh, James Monosov, Ilya E. McAlonan, Kerry Berman, Rebecca Smith, Mitchell K. Cao, Vania Wang, Kuan H. Boyden, Edward Stuart Wurtz, Robert H. Optogenetic Inactivation Modifies Monkey Visuomotor Behavior |
| title | Optogenetic Inactivation Modifies Monkey Visuomotor Behavior |
| title_full | Optogenetic Inactivation Modifies Monkey Visuomotor Behavior |
| title_fullStr | Optogenetic Inactivation Modifies Monkey Visuomotor Behavior |
| title_full_unstemmed | Optogenetic Inactivation Modifies Monkey Visuomotor Behavior |
| title_short | Optogenetic Inactivation Modifies Monkey Visuomotor Behavior |
| title_sort | optogenetic inactivation modifies monkey visuomotor behavior |
| url | http://hdl.handle.net/1721.1/103646 https://orcid.org/0000-0002-0419-3351 |
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