Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, February 2016.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2016
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| Online Access: | http://hdl.handle.net/1721.1/103709 |
| _version_ | 1811091794607210496 |
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| author | Guo, Syuan-Ming |
| author2 | Mark Bathe. |
| author_facet | Mark Bathe. Guo, Syuan-Ming |
| author_sort | Guo, Syuan-Ming |
| collection | MIT |
| description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, February 2016. |
| first_indexed | 2024-09-23T15:08:08Z |
| format | Thesis |
| id | mit-1721.1/103709 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T15:08:08Z |
| publishDate | 2016 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1037092019-04-10T14:43:47Z Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization Guo, Syuan-Ming Mark Bathe. Massachusetts Institute of Technology. Department of Chemistry. Massachusetts Institute of Technology. Department of Chemistry. Chemistry. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, February 2016. Cataloged from PDF version of thesis. "February 2016." Includes bibliographical references. Fluorescence fluctuation spectroscopy and microscopy have been powerful tools for studying molecular organization and dynamics in cells. Fluorescence Correlation Spectroscopy (FCS) has been widely applied to probing molecular dynamics in live cells with single molecule sensitivity and the ability to resolve local molecular concentrations, aggregation states, and transport mechanisms. Despite the broad utility of FCS, interpretation of cellular FCS data is often confounded by the heterogeneity in the underlying biological process and the low signal-to-noise ratio. Systematic data evaluation and interpretation become even more challenging for imaging FCS, where hundreds to thousands of FCS curves are generated in a single measurement. This thesis presents an objective Bayesian inference procedure for testing multiple competing FCS models. Bayesian inference determines model probabilities by considering the probability distributions over the full range of parameter values, thereby naturally penalizes model complexity and prevents over-fitting. We applied this procedure to imaging FCS data in order to resolve hIAPP-induced microdomain spatial organization and temporal dynamics in the cell membrane. Our analysis resolved the temporal evolution of multiple diffusing species in the spatially heterogeneous cell membrane, lending support to the "carpet model" for the association mode of hIAPP aggregates with the plasma membrane. Finally, we presented a fluctuation-based microscopy approach, Points Accumulation for Imaging in Nanoscale Topography (PAINT), that enables highly multiplexed super-resolution imaging of synaptic proteins. We employed DNA-PAINT to resolve nano-scale organization of seven targets simultaneously including synaptic proteins and cytoskeletal markers. These approaches demonstrated the broad applicability of fluorescence fluctuation spectroscopy and microscopy in resolving molecular dynamics and organization in cells. by Syuan-Ming Guo. Ph. D. 2016-07-18T20:03:14Z 2016-07-18T20:03:14Z 2015 2016 Thesis http://hdl.handle.net/1721.1/103709 953261914 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 139 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemistry. Guo, Syuan-Ming Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization |
| title | Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization |
| title_full | Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization |
| title_fullStr | Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization |
| title_full_unstemmed | Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization |
| title_short | Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization |
| title_sort | fluorescence fluctuation spectroscopy and microscopy application to cellular molecular dynamics and organization |
| topic | Chemistry. |
| url | http://hdl.handle.net/1721.1/103709 |
| work_keys_str_mv | AT guosyuanming fluorescencefluctuationspectroscopyandmicroscopyapplicationtocellularmoleculardynamicsandorganization |