Single-Cell Analysis Reveals that Expression of Nanog Is Biallelic and Equally Variable as that of Other Pluripotency Factors in Mouse ESCs
The homeodomain transcription factor Nanog is a central part of the core pluripotency transcriptional network and plays a critical role in embryonic stem cell (ESC) self-renewal. Several reports have suggested that Nanog expression is allelically regulated and that transient downregulation of Nanog...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2016
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| Online Access: | http://hdl.handle.net/1721.1/103900 |
| _version_ | 1811081875150602240 |
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| author | Wang, Haoyi Cheng, Albert Wu Katz, Yarden Buganim, Yosef Jaenisch, Rudolf Faddah, Dina A. |
| author2 | Massachusetts Institute of Technology. Computational and Systems Biology Program |
| author_facet | Massachusetts Institute of Technology. Computational and Systems Biology Program Wang, Haoyi Cheng, Albert Wu Katz, Yarden Buganim, Yosef Jaenisch, Rudolf Faddah, Dina A. |
| author_sort | Wang, Haoyi |
| collection | MIT |
| description | The homeodomain transcription factor Nanog is a central part of the core pluripotency transcriptional network and plays a critical role in embryonic stem cell (ESC) self-renewal. Several reports have suggested that Nanog expression is allelically regulated and that transient downregulation of Nanog in a subset of pluripotent cells predisposes them toward differentiation. Using single-cell gene expression analyses combined with different reporters for the two alleles of Nanog, we show that Nanog is biallelically expressed in ESCs independently of culture condition. We also show that the overall variation in endogenous Nanog expression in ESCs is very similar to that of several other pluripotency markers. Our analysis suggests that reporter-based studies of gene expression in pluripotent cells can be significantly influenced by the gene-targeting strategy and genetic background employed. |
| first_indexed | 2024-09-23T11:53:47Z |
| format | Article |
| id | mit-1721.1/103900 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:53:47Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1039002022-10-01T06:48:07Z Single-Cell Analysis Reveals that Expression of Nanog Is Biallelic and Equally Variable as that of Other Pluripotency Factors in Mouse ESCs Wang, Haoyi Cheng, Albert Wu Katz, Yarden Buganim, Yosef Jaenisch, Rudolf Faddah, Dina A. Massachusetts Institute of Technology. Computational and Systems Biology Program Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Whitehead Institute for Biomedical Research Faddah, Dina Adel Cheng, Albert Wu Katz, Yarden Jaenisch, Rudolf The homeodomain transcription factor Nanog is a central part of the core pluripotency transcriptional network and plays a critical role in embryonic stem cell (ESC) self-renewal. Several reports have suggested that Nanog expression is allelically regulated and that transient downregulation of Nanog in a subset of pluripotent cells predisposes them toward differentiation. Using single-cell gene expression analyses combined with different reporters for the two alleles of Nanog, we show that Nanog is biallelically expressed in ESCs independently of culture condition. We also show that the overall variation in endogenous Nanog expression in ESCs is very similar to that of several other pluripotency markers. Our analysis suggests that reporter-based studies of gene expression in pluripotent cells can be significantly influenced by the gene-targeting strategy and genetic background employed. National Science Foundation (U.S.) (Graduate Research Fellowship) Whitehead Institute for Biomedical Research (Jerome and Florence Brill Graduate Student Fellowship) Croucher Foundation (Research Fellowship) Virginia and Daniel K. Ludwig Graduate Fellowship National Institutes of Health (U.S.) ((NIH) Kirschstein National Research Service Award (1 F32 GM099153-01A1)) National Institutes of Health (U.S.) (NIH grant HD 045022) National Institutes of Health (U.S.) (NIH grant R37CA084198) 2016-08-11T18:15:31Z 2016-08-11T18:15:31Z 2013-07 2013-03 Article http://purl.org/eprint/type/JournalArticle 19345909 http://hdl.handle.net/1721.1/103900 Faddah, Dina A., Haoyi Wang, Albert Wu Cheng, Yarden Katz, Yosef Buganim, and Rudolf Jaenisch. “Single-Cell Analysis Reveals That Expression of Nanog Is Biallelic and Equally Variable as That of Other Pluripotency Factors in Mouse ESCs.” Cell Stem Cell 13, no. 1 (July 2013): 23–29. en_US http://dx.doi.org/10.1016/j.stem.2013.04.019 Cell Stem Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Wang, Haoyi Cheng, Albert Wu Katz, Yarden Buganim, Yosef Jaenisch, Rudolf Faddah, Dina A. Single-Cell Analysis Reveals that Expression of Nanog Is Biallelic and Equally Variable as that of Other Pluripotency Factors in Mouse ESCs |
| title | Single-Cell Analysis Reveals that Expression of Nanog Is Biallelic and Equally Variable as that of Other Pluripotency Factors in Mouse ESCs |
| title_full | Single-Cell Analysis Reveals that Expression of Nanog Is Biallelic and Equally Variable as that of Other Pluripotency Factors in Mouse ESCs |
| title_fullStr | Single-Cell Analysis Reveals that Expression of Nanog Is Biallelic and Equally Variable as that of Other Pluripotency Factors in Mouse ESCs |
| title_full_unstemmed | Single-Cell Analysis Reveals that Expression of Nanog Is Biallelic and Equally Variable as that of Other Pluripotency Factors in Mouse ESCs |
| title_short | Single-Cell Analysis Reveals that Expression of Nanog Is Biallelic and Equally Variable as that of Other Pluripotency Factors in Mouse ESCs |
| title_sort | single cell analysis reveals that expression of nanog is biallelic and equally variable as that of other pluripotency factors in mouse escs |
| url | http://hdl.handle.net/1721.1/103900 |
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