Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator
The potential of plant oils as sole carbon sources for production of P(3HB-co-3HHx) copolymer containing a high 3HHx monomer fraction using the recombinant Cupriavidus necator strain Re2160/pCB113 has been investigated. Various types and concentrations of plant oils were evaluated for efficient conv...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2016
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| Online Access: | http://hdl.handle.net/1721.1/103938 https://orcid.org/0000-0002-1015-1270 https://orcid.org/0000-0002-6671-5987 |
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| author | Wong, Yoke-Ming Brigham, Christopher J. Sudesh, Kumar Rha, Chokyun Sinskey, Anthony J |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Wong, Yoke-Ming Brigham, Christopher J. Sudesh, Kumar Rha, Chokyun Sinskey, Anthony J |
| author_sort | Wong, Yoke-Ming |
| collection | MIT |
| description | The potential of plant oils as sole carbon sources for production of P(3HB-co-3HHx) copolymer containing a high 3HHx monomer fraction using the recombinant Cupriavidus necator strain Re2160/pCB113 has been investigated. Various types and concentrations of plant oils were evaluated for efficient conversion of P(3HB-co-3HHx) copolymer. Crude palm kernel oil (CPKO) at a concentration of 2.5 g/L was found to be most suitable for production of copolymer with a 3HHx content of approximately 70 mol%. The time profile of these cells was also examined in order to study the trend of 3HHx monomer incorporation, PHA production and PHA synthase activity. [superscript 1]H NMR and [superscript 13]C NMR analyses confirmed the presence of P(3HB-co-3HHx) copolymer containing a high 3HHx monomer fraction, in which monomers were not randomly distributed. The results of various characterization analyses revealed that the copolymers containing a high 3HHx monomer fraction demonstrated soft and flexible mechanical properties. |
| first_indexed | 2024-09-23T10:30:52Z |
| format | Article |
| id | mit-1721.1/103938 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:30:52Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1039382022-09-30T21:35:37Z Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator Wong, Yoke-Ming Brigham, Christopher J. Sudesh, Kumar Rha, Chokyun Sinskey, Anthony J Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Biomaterials Science and Engineering Laboratory Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Engineering Systems Division Sinskey, Anthony J. Brigham, Christopher J. Rha, ChoKyun Sinskey, Anthony J. The potential of plant oils as sole carbon sources for production of P(3HB-co-3HHx) copolymer containing a high 3HHx monomer fraction using the recombinant Cupriavidus necator strain Re2160/pCB113 has been investigated. Various types and concentrations of plant oils were evaluated for efficient conversion of P(3HB-co-3HHx) copolymer. Crude palm kernel oil (CPKO) at a concentration of 2.5 g/L was found to be most suitable for production of copolymer with a 3HHx content of approximately 70 mol%. The time profile of these cells was also examined in order to study the trend of 3HHx monomer incorporation, PHA production and PHA synthase activity. [superscript 1]H NMR and [superscript 13]C NMR analyses confirmed the presence of P(3HB-co-3HHx) copolymer containing a high 3HHx monomer fraction, in which monomers were not randomly distributed. The results of various characterization analyses revealed that the copolymers containing a high 3HHx monomer fraction demonstrated soft and flexible mechanical properties. Malaysia. Ministry of Science, Technology and Innovation (MOSTI Techno Fund) Universiti Sains Malaysia (USM Fellowship) 2016-08-16T18:26:54Z 2016-08-16T18:26:54Z 2012-10 2012-06 Article http://purl.org/eprint/type/JournalArticle 09608524 http://hdl.handle.net/1721.1/103938 Wong, Yoke-Ming, Christopher J. Brigham, ChoKyun Rha, Anthony J. Sinskey, and Kumar Sudesh. “Biosynthesis and Characterization of Polyhydroxyalkanoate Containing High 3-Hydroxyhexanoate Monomer Fraction from Crude Palm Kernel Oil by Recombinant Cupriavidus Necator.” Bioresource Technology 121 (October 2012): 320–327. https://orcid.org/0000-0002-1015-1270 https://orcid.org/0000-0002-6671-5987 en_US http://dx.doi.org/10.1016/j.biortech.2012.07.015 Bioresource Technology Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier Prof. Sinskey |
| spellingShingle | Wong, Yoke-Ming Brigham, Christopher J. Sudesh, Kumar Rha, Chokyun Sinskey, Anthony J Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator |
| title | Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator |
| title_full | Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator |
| title_fullStr | Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator |
| title_full_unstemmed | Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator |
| title_short | Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator |
| title_sort | biosynthesis and characterization of polyhydroxyalkanoate containing high 3 hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant cupriavidus necator |
| url | http://hdl.handle.net/1721.1/103938 https://orcid.org/0000-0002-1015-1270 https://orcid.org/0000-0002-6671-5987 |
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