Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice
Low-level laser (light) therapy (LLLT) promotes wound healing, reduces pain and inflammation, and prevents tissue death. Studies have explored the effects of various radiant exposures on the effect of LLLT; however, studies of wavelength dependency in in vivo models are less common. In the present s...
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| Format: | Article |
| Language: | English |
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Springer London
2016
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| Online Access: | http://hdl.handle.net/1721.1/104348 |
| _version_ | 1826198789314052096 |
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| author | Gupta, Asheesh Dai, Tianhong Hamblin, Michael R |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Gupta, Asheesh Dai, Tianhong Hamblin, Michael R |
| author_sort | Gupta, Asheesh |
| collection | MIT |
| description | Low-level laser (light) therapy (LLLT) promotes wound healing, reduces pain and inflammation, and prevents tissue death. Studies have explored the effects of various radiant exposures on the effect of LLLT; however, studies of wavelength dependency in in vivo models are less common. In the present study, the healing effects of LLLT mediated by different wavelengths of light in the red and near-infrared (NIR) wavelength regions (635, 730, 810, and 980 nm) delivered at constant fluence (4 J/cm2) and fluence rate (10 mW/cm2) were evaluated in a mouse model of partial-thickness dermal abrasion. Wavelengths of 635 and 810 nm were found to be effective in promoting the healing of dermal abrasions. However, treatment using 730- and 980-nm wavelengths showed no sign of stimulated healing. Healing was maximally augmented in mice treated with an 810-nm wavelength, as evidenced by significant wound area reduction (p < 0.05), enhanced collagen accumulation, and complete re-epithelialization as compared to other wavelengths and non-illuminated controls. Significant acceleration of re-epithelialization and cellular proliferation revealed by immunofluorescence staining for cytokeratin-14 and proliferating cell nuclear antigen (p < 0.05) was evident in the 810-nm wavelength compared with other groups. Photobiomodulation mediated by red (635 nm) and NIR (810 nm) light suggests that the biological response of the wound tissue depends on the wavelength employed. The effectiveness of 810-nm wavelength agrees with previous publications and, together with the partial effectiveness of 635 nm and the ineffectiveness of 730 and 980 nm wavelengths, can be explained by the absorption spectrum of cytochrome c oxidase, the candidate mitochondrial chromophore in LLLT. |
| first_indexed | 2024-09-23T11:10:01Z |
| format | Article |
| id | mit-1721.1/104348 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:10:01Z |
| publishDate | 2016 |
| publisher | Springer London |
| record_format | dspace |
| spelling | mit-1721.1/1043482022-10-01T01:45:56Z Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice Gupta, Asheesh Dai, Tianhong Hamblin, Michael R Harvard University--MIT Division of Health Sciences and Technology Hamblin, Michael R Low-level laser (light) therapy (LLLT) promotes wound healing, reduces pain and inflammation, and prevents tissue death. Studies have explored the effects of various radiant exposures on the effect of LLLT; however, studies of wavelength dependency in in vivo models are less common. In the present study, the healing effects of LLLT mediated by different wavelengths of light in the red and near-infrared (NIR) wavelength regions (635, 730, 810, and 980 nm) delivered at constant fluence (4 J/cm2) and fluence rate (10 mW/cm2) were evaluated in a mouse model of partial-thickness dermal abrasion. Wavelengths of 635 and 810 nm were found to be effective in promoting the healing of dermal abrasions. However, treatment using 730- and 980-nm wavelengths showed no sign of stimulated healing. Healing was maximally augmented in mice treated with an 810-nm wavelength, as evidenced by significant wound area reduction (p < 0.05), enhanced collagen accumulation, and complete re-epithelialization as compared to other wavelengths and non-illuminated controls. Significant acceleration of re-epithelialization and cellular proliferation revealed by immunofluorescence staining for cytokeratin-14 and proliferating cell nuclear antigen (p < 0.05) was evident in the 810-nm wavelength compared with other groups. Photobiomodulation mediated by red (635 nm) and NIR (810 nm) light suggests that the biological response of the wound tissue depends on the wavelength employed. The effectiveness of 810-nm wavelength agrees with previous publications and, together with the partial effectiveness of 635 nm and the ineffectiveness of 730 and 980 nm wavelengths, can be explained by the absorption spectrum of cytochrome c oxidase, the candidate mitochondrial chromophore in LLLT. United States. National Institutes of Health 2016-09-16T22:48:02Z 2016-09-16T22:48:02Z 2013-04 2012-06 2016-08-18T15:21:08Z Article http://purl.org/eprint/type/JournalArticle 0268-8921 1435-604X http://hdl.handle.net/1721.1/104348 Gupta, Asheesh, Tianhong Dai, and Michael R. Hamblin. “Effect of Red and near-Infrared Wavelengths on Low-Level Laser (Light) Therapy-Induced Healing of Partial-Thickness Dermal Abrasion in Mice.” Lasers in Medical Science 29.1 (2014): 257–265. en http://dx.doi.org/10.1007/s10103-013-1319-0 Lasers in Medical Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. Springer-Verlag London application/pdf Springer London Springer London |
| spellingShingle | Gupta, Asheesh Dai, Tianhong Hamblin, Michael R Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice |
| title | Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice |
| title_full | Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice |
| title_fullStr | Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice |
| title_full_unstemmed | Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice |
| title_short | Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice |
| title_sort | effect of red and near infrared wavelengths on low level laser light therapy induced healing of partial thickness dermal abrasion in mice |
| url | http://hdl.handle.net/1721.1/104348 |
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