Microfluidic fabrication of cell adhesive chitosan microtubes
Chitosan has been used as a scaffolding material in tissue engineering due to its mechanical properties and biocompatibility. With increased appreciation of the effect of micro- and nanoscale environments on cellular behavior, there is increased emphasis on generating microfabricated chitosan struct...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer US
2016
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| Online Access: | http://hdl.handle.net/1721.1/104880 https://orcid.org/0000-0002-0284-0201 |
| _version_ | 1811088784205283328 |
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| author | Lee, Sang-Hoon Lee, Kwang Ho Lee, Dong Hwan Oh, Jonghyun Kim, Keekyoung Won, Sung Wook Selimovic, Seila Bae, Hojae Cha, Chaenyung Gaharwar, Akhilesh Khademhosseini, Ali |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Lee, Sang-Hoon Lee, Kwang Ho Lee, Dong Hwan Oh, Jonghyun Kim, Keekyoung Won, Sung Wook Selimovic, Seila Bae, Hojae Cha, Chaenyung Gaharwar, Akhilesh Khademhosseini, Ali |
| author_sort | Lee, Sang-Hoon |
| collection | MIT |
| description | Chitosan has been used as a scaffolding material in tissue engineering due to its mechanical properties and biocompatibility. With increased appreciation of the effect of micro- and nanoscale environments on cellular behavior, there is increased emphasis on generating microfabricated chitosan structures. Here we employed a microfluidic coaxial flow-focusing system to generate cell adhesive chitosan microtubes of controlled sizes by modifying the flow rates of a chitosan pre-polymer solution and phosphate buffered saline (PBS). The microtubes were extruded from a glass capillary with a 300 μm inner diameter. After ionic crosslinking with sodium tripolyphosphate (TPP), fabricated microtubes had inner and outer diameter ranges of 70–150 μm and 120–185 μm. Computational simulation validated the controlled size of microtubes and cell attachment. To enhance cell adhesiveness on the microtubes, we mixed gelatin with the chitosan pre-polymer solution. During the fabrication of microtubes, fibroblasts suspended in core PBS flow adhered to the inner surface of chitosan-gelatin microtubes. To achieve physiological pH values, we adjusted pH values of chiotsan pre-polymer solution and TPP. In particular, we were able to improve cell viability to 92 % with pH values of 5.8 and 7.4 for chitosan and TPP solution respectively. Cell culturing for three days showed that the addition of the gelatin enhanced cell spreading and proliferation inside the chitosan-gelatin microtubes. The microfluidic fabrication method for ionically crosslinked chitosan microtubes at physiological pH can be compatible with a variety of cells and used as a versatile platform for microengineered tissue engineering. |
| first_indexed | 2024-09-23T14:07:29Z |
| format | Article |
| id | mit-1721.1/104880 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:07:29Z |
| publishDate | 2016 |
| publisher | Springer US |
| record_format | dspace |
| spelling | mit-1721.1/1048802022-10-01T19:21:49Z Microfluidic fabrication of cell adhesive chitosan microtubes Lee, Sang-Hoon Lee, Kwang Ho Lee, Dong Hwan Oh, Jonghyun Kim, Keekyoung Won, Sung Wook Selimovic, Seila Bae, Hojae Cha, Chaenyung Gaharwar, Akhilesh Khademhosseini, Ali Harvard University--MIT Division of Health Sciences and Technology Koch Institute for Integrative Cancer Research at MIT Oh, Jonghyun Kim, Keekyoung Won, Sung Wook Selimovic, Seila Bae, Hojae Cha, Chaenyung Gaharwar, Akhilesh Khademhosseini, Alireza Chitosan has been used as a scaffolding material in tissue engineering due to its mechanical properties and biocompatibility. With increased appreciation of the effect of micro- and nanoscale environments on cellular behavior, there is increased emphasis on generating microfabricated chitosan structures. Here we employed a microfluidic coaxial flow-focusing system to generate cell adhesive chitosan microtubes of controlled sizes by modifying the flow rates of a chitosan pre-polymer solution and phosphate buffered saline (PBS). The microtubes were extruded from a glass capillary with a 300 μm inner diameter. After ionic crosslinking with sodium tripolyphosphate (TPP), fabricated microtubes had inner and outer diameter ranges of 70–150 μm and 120–185 μm. Computational simulation validated the controlled size of microtubes and cell attachment. To enhance cell adhesiveness on the microtubes, we mixed gelatin with the chitosan pre-polymer solution. During the fabrication of microtubes, fibroblasts suspended in core PBS flow adhered to the inner surface of chitosan-gelatin microtubes. To achieve physiological pH values, we adjusted pH values of chiotsan pre-polymer solution and TPP. In particular, we were able to improve cell viability to 92 % with pH values of 5.8 and 7.4 for chitosan and TPP solution respectively. Cell culturing for three days showed that the addition of the gelatin enhanced cell spreading and proliferation inside the chitosan-gelatin microtubes. The microfluidic fabrication method for ionically crosslinked chitosan microtubes at physiological pH can be compatible with a variety of cells and used as a versatile platform for microengineered tissue engineering. National Science Foundation (U.S.) (CAREER Award DMR 0847287) United States. Office of Naval Research (Young National Investigator Award) National Institutes of Health (U.S.) (Grants HL092836, EB008392, DE021468, AR057837, EB012597, HL099073, and GM095906) United States. Army. Corps of Engineers Natural Sciences and Engineering Research Council of Canada. Postdoctoral Fellowship Innovative Med Tech MIT-Portugal Program (Grant (MPP-09Call-Langer-47) 2016-10-20T16:53:43Z 2016-10-20T16:53:43Z 2013-01 2016-08-18T15:44:24Z Article http://purl.org/eprint/type/JournalArticle 1387-2176 1572-8781 http://hdl.handle.net/1721.1/104880 Oh, Jonghyun et al. “Microfluidic Fabrication of Cell Adhesive Chitosan Microtubes.” Biomedical Microdevices 15.3 (2013): 465–472. https://orcid.org/0000-0002-0284-0201 en http://dx.doi.org/10.1007/s10544-013-9746-z Biomedical Microdevices Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. Springer Science+Business Media New York application/pdf Springer US Springer US |
| spellingShingle | Lee, Sang-Hoon Lee, Kwang Ho Lee, Dong Hwan Oh, Jonghyun Kim, Keekyoung Won, Sung Wook Selimovic, Seila Bae, Hojae Cha, Chaenyung Gaharwar, Akhilesh Khademhosseini, Ali Microfluidic fabrication of cell adhesive chitosan microtubes |
| title | Microfluidic fabrication of cell adhesive chitosan microtubes |
| title_full | Microfluidic fabrication of cell adhesive chitosan microtubes |
| title_fullStr | Microfluidic fabrication of cell adhesive chitosan microtubes |
| title_full_unstemmed | Microfluidic fabrication of cell adhesive chitosan microtubes |
| title_short | Microfluidic fabrication of cell adhesive chitosan microtubes |
| title_sort | microfluidic fabrication of cell adhesive chitosan microtubes |
| url | http://hdl.handle.net/1721.1/104880 https://orcid.org/0000-0002-0284-0201 |
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