Adipose tissue macrophages in non-rodent mammals: a comparative study
The stromal vascular fraction (SVF) of adipose tissue in rodents and primates contains mesenchymal stem cells and immune cells. SVF cells have complex metabolic, immune and endocrine functions with biomedical impact. However, in other mammals, the amount of data on SVF stem cells is negligible and w...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer-Verlag
2016
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| Online Access: | http://hdl.handle.net/1721.1/104890 |
| _version_ | 1826208063531515904 |
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| author | Ampem, Grace Bacsadi, Árpád Balogh, Lajos Schmidt, Susanne Thuróczy, Julianna Röszer, Tamás Azegrouz, Hind |
| author2 | Massachusetts Institute of Technology. Research Laboratory of Electronics |
| author_facet | Massachusetts Institute of Technology. Research Laboratory of Electronics Ampem, Grace Bacsadi, Árpád Balogh, Lajos Schmidt, Susanne Thuróczy, Julianna Röszer, Tamás Azegrouz, Hind |
| author_sort | Ampem, Grace |
| collection | MIT |
| description | The stromal vascular fraction (SVF) of adipose tissue in rodents and primates contains mesenchymal stem cells and immune cells. SVF cells have complex metabolic, immune and endocrine functions with biomedical impact. However, in other mammals, the amount of data on SVF stem cells is negligible and whether the SVF hosts immune cells is unknown. In this study, we show that the SVF is rich in immune cells, with a dominance of adipose tissue macrophages (ATMs) in cattle (Bos primigenius taurus), domestic goat (Capra aegagrus hircus), domestic sheep (Ovis aries), domestic cat (Felis catus) and domestic dog (Canis familiaris). ATMs of these species are granulated lysosome-rich cells with lamellipodial protrusions and express the lysosome markers acid phosphatase 5 (ACP-5) and Mac-3/Lamp-2. Using ACP-5 and Mac-3/Lamp-2 as markers, we additionally detected ATMs in other species, such as the domestic horse (Equus ferus caballus), wild boar (Sus scrofa) and red fox (Vulpes vulpes). Feline and canine ATMs also express the murine macrophage marker F4/80 antigen. In the lean condition, the alternative macrophage activation marker CD206 is expressed by feline and canine ATMs and arginase-1 by feline ATMs. Obesity is associated with interleukin-6 and interferon gamma expression and with overt tyrosine nitration in both feline and canine ATMs. This resembles the obesity-induced phenotype switch of murine and human ATMs. Thus, we show, for the first time, that the presence of ATMs is a general trait of mammals. The interaction between the adipose cells and SVF immune cells might be evolutionarily conserved among mammals. |
| first_indexed | 2024-09-23T13:59:55Z |
| format | Article |
| id | mit-1721.1/104890 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:59:55Z |
| publishDate | 2016 |
| publisher | Springer-Verlag |
| record_format | dspace |
| spelling | mit-1721.1/1048902022-10-01T18:28:43Z Adipose tissue macrophages in non-rodent mammals: a comparative study Ampem, Grace Bacsadi, Árpád Balogh, Lajos Schmidt, Susanne Thuróczy, Julianna Röszer, Tamás Azegrouz, Hind Massachusetts Institute of Technology. Research Laboratory of Electronics Azegrouz, Hind The stromal vascular fraction (SVF) of adipose tissue in rodents and primates contains mesenchymal stem cells and immune cells. SVF cells have complex metabolic, immune and endocrine functions with biomedical impact. However, in other mammals, the amount of data on SVF stem cells is negligible and whether the SVF hosts immune cells is unknown. In this study, we show that the SVF is rich in immune cells, with a dominance of adipose tissue macrophages (ATMs) in cattle (Bos primigenius taurus), domestic goat (Capra aegagrus hircus), domestic sheep (Ovis aries), domestic cat (Felis catus) and domestic dog (Canis familiaris). ATMs of these species are granulated lysosome-rich cells with lamellipodial protrusions and express the lysosome markers acid phosphatase 5 (ACP-5) and Mac-3/Lamp-2. Using ACP-5 and Mac-3/Lamp-2 as markers, we additionally detected ATMs in other species, such as the domestic horse (Equus ferus caballus), wild boar (Sus scrofa) and red fox (Vulpes vulpes). Feline and canine ATMs also express the murine macrophage marker F4/80 antigen. In the lean condition, the alternative macrophage activation marker CD206 is expressed by feline and canine ATMs and arginase-1 by feline ATMs. Obesity is associated with interleukin-6 and interferon gamma expression and with overt tyrosine nitration in both feline and canine ATMs. This resembles the obesity-induced phenotype switch of murine and human ATMs. Thus, we show, for the first time, that the presence of ATMs is a general trait of mammals. The interaction between the adipose cells and SVF immune cells might be evolutionarily conserved among mammals. University of Ulm 2016-10-20T19:32:23Z 2016-10-20T19:32:23Z 2015-08 2015-03 2016-08-18T15:27:53Z Article http://purl.org/eprint/type/JournalArticle 0302-766X 1432-0878 http://hdl.handle.net/1721.1/104890 Ampem, Grace, Hind Azegrouz, Arpad Bacsadi, Lajos Balogh, Susanne Schmidt, Julianna Thuroczy, and Tamas Roszer. "Adipose tissue macrophages in non-rodent mammals: a comparative study." Cell and Tissue Research, vol. 363, no. 2, August 2015, pp. 461-478. en http://dx.doi.org/10.1007/s00441-015-2253-1 Cell and Tissue Research Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. Springer-Verlag Berlin Heidelberg application/pdf Springer-Verlag Springer Berlin Heidelberg |
| spellingShingle | Ampem, Grace Bacsadi, Árpád Balogh, Lajos Schmidt, Susanne Thuróczy, Julianna Röszer, Tamás Azegrouz, Hind Adipose tissue macrophages in non-rodent mammals: a comparative study |
| title | Adipose tissue macrophages in non-rodent mammals: a comparative study |
| title_full | Adipose tissue macrophages in non-rodent mammals: a comparative study |
| title_fullStr | Adipose tissue macrophages in non-rodent mammals: a comparative study |
| title_full_unstemmed | Adipose tissue macrophages in non-rodent mammals: a comparative study |
| title_short | Adipose tissue macrophages in non-rodent mammals: a comparative study |
| title_sort | adipose tissue macrophages in non rodent mammals a comparative study |
| url | http://hdl.handle.net/1721.1/104890 |
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