Hedgehog signaling regulates gene expression in planarian glia
Hedgehog signaling is critical for vertebrate central nervous system (CNS) development, but its role in CNS biology in other organisms is poorly characterized. In the planarian Schmidtea mediterranea, hedgehog (hh) is expressed in medial cephalic ganglia neurons, suggesting a possible role in CNS ma...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
eLife Sciences Publications, Ltd.
2016
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| Online Access: | http://hdl.handle.net/1721.1/105106 https://orcid.org/0000-0002-5569-333X |
| _version_ | 1826195791603040256 |
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| author | Scimone, M Lucila Clandinin, Thomas R Wang, Irving E. Reddien, Peter Lapan, Sylvain W. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Scimone, M Lucila Clandinin, Thomas R Wang, Irving E. Reddien, Peter Lapan, Sylvain W. |
| author_sort | Scimone, M Lucila |
| collection | MIT |
| description | Hedgehog signaling is critical for vertebrate central nervous system (CNS) development, but its role in CNS biology in other organisms is poorly characterized. In the planarian Schmidtea mediterranea, hedgehog (hh) is expressed in medial cephalic ganglia neurons, suggesting a possible role in CNS maintenance or regeneration. We performed RNA sequencing of planarian brain tissue following RNAi of hh and patched (ptc), which encodes the Hh receptor. Two misregulated genes, intermediate filament-1 (if-1) and calamari (cali), were expressed in a previously unidentified non-neural CNS cell type. These cells expressed orthologs of astrocyte-associated genes involved in neurotransmitter uptake and metabolism, and extended processes enveloping regions of high synapse concentration. We propose that these cells are planarian glia. Planarian glia were distributed broadly, but only expressed if-1 and cali in the neuropil near hh[superscript +] neurons. Planarian glia and their regulation by Hedgehog signaling present a novel tractable system for dissection of glia biology. |
| first_indexed | 2024-09-23T10:15:29Z |
| format | Article |
| id | mit-1721.1/105106 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:15:29Z |
| publishDate | 2016 |
| publisher | eLife Sciences Publications, Ltd. |
| record_format | dspace |
| spelling | mit-1721.1/1051062022-09-30T19:59:00Z Hedgehog signaling regulates gene expression in planarian glia Scimone, M Lucila Clandinin, Thomas R Wang, Irving E. Reddien, Peter Lapan, Sylvain W. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Wang, Irving E. Lapan, Sylvain William Reddien, Peter Hedgehog signaling is critical for vertebrate central nervous system (CNS) development, but its role in CNS biology in other organisms is poorly characterized. In the planarian Schmidtea mediterranea, hedgehog (hh) is expressed in medial cephalic ganglia neurons, suggesting a possible role in CNS maintenance or regeneration. We performed RNA sequencing of planarian brain tissue following RNAi of hh and patched (ptc), which encodes the Hh receptor. Two misregulated genes, intermediate filament-1 (if-1) and calamari (cali), were expressed in a previously unidentified non-neural CNS cell type. These cells expressed orthologs of astrocyte-associated genes involved in neurotransmitter uptake and metabolism, and extended processes enveloping regions of high synapse concentration. We propose that these cells are planarian glia. Planarian glia were distributed broadly, but only expressed if-1 and cali in the neuropil near hh[superscript +] neurons. Planarian glia and their regulation by Hedgehog signaling present a novel tractable system for dissection of glia biology. National Institutes of Health (U.S.) (NIH (R01GM080639)) Howard Hughes Medical Institute (Investigator) 2016-10-26T20:26:18Z 2016-10-26T20:26:18Z 2016-09 2016-04 Article http://purl.org/eprint/type/JournalArticle 2050-084X http://hdl.handle.net/1721.1/105106 Wang, Irving E., Sylvain W. Lapan, M. Lucila Scimone, Thomas R. Clandinin, and Peter W. Reddien. “Hedgehog Signaling Regulates Gene Expression in Planarian Glia.” eLife 5 (September 9, 2016). p.1-26. https://orcid.org/0000-0002-5569-333X en_US http://dx.doi.org/10.7554/eLife.16996 eLife Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf eLife Sciences Publications, Ltd. eLife |
| spellingShingle | Scimone, M Lucila Clandinin, Thomas R Wang, Irving E. Reddien, Peter Lapan, Sylvain W. Hedgehog signaling regulates gene expression in planarian glia |
| title | Hedgehog signaling regulates gene expression in planarian glia |
| title_full | Hedgehog signaling regulates gene expression in planarian glia |
| title_fullStr | Hedgehog signaling regulates gene expression in planarian glia |
| title_full_unstemmed | Hedgehog signaling regulates gene expression in planarian glia |
| title_short | Hedgehog signaling regulates gene expression in planarian glia |
| title_sort | hedgehog signaling regulates gene expression in planarian glia |
| url | http://hdl.handle.net/1721.1/105106 https://orcid.org/0000-0002-5569-333X |
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