Three-dimensional manipulation of single cells using surface acoustic waves
The ability of surface acoustic waves to trap and manipulate micrometer-scale particles and biological cells has led to many applications involving “acoustic tweezers” in biology, chemistry, engineering, and medicine. Here, we present 3D acoustic tweezers, which use surface acoustic waves to create...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
| Published: |
National Academy of Sciences (U.S.)
2016
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| Online Access: | http://hdl.handle.net/1721.1/105140 |
| _version_ | 1826206703409954816 |
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| author | Guo, Feng Mao, Zhangming Chen, Yuchao Xie, Zhiwei Lata, James P. Li, Peng Ren, Liqiang Liu, Jiayang Yang, Jian Dao, Ming Suresh, Subra Huang, Tony Jun |
| author2 | Massachusetts Institute of Technology. Center for Materials Science and Engineering |
| author_facet | Massachusetts Institute of Technology. Center for Materials Science and Engineering Guo, Feng Mao, Zhangming Chen, Yuchao Xie, Zhiwei Lata, James P. Li, Peng Ren, Liqiang Liu, Jiayang Yang, Jian Dao, Ming Suresh, Subra Huang, Tony Jun |
| author_sort | Guo, Feng |
| collection | MIT |
| description | The ability of surface acoustic waves to trap and manipulate micrometer-scale particles and biological cells has led to many applications involving “acoustic tweezers” in biology, chemistry, engineering, and medicine. Here, we present 3D acoustic tweezers, which use surface acoustic waves to create 3D trapping nodes for the capture and manipulation of microparticles and cells along three mutually orthogonal axes. In this method, we use standing-wave phase shifts to move particles or cells in-plane, whereas the amplitude of acoustic vibrations is used to control particle motion along an orthogonal plane. We demonstrate, through controlled experiments guided by simulations, how acoustic vibrations result in micromanipulations in a microfluidic chamber by invoking physical principles that underlie the formation and regulation of complex, volumetric trapping nodes of particles and biological cells. We further show how 3D acoustic tweezers can be used to pick up, translate, and print single cells and cell assemblies to create 2D and 3D structures in a precise, noninvasive, label-free, and contact-free manner. |
| first_indexed | 2024-09-23T13:36:56Z |
| format | Article |
| id | mit-1721.1/105140 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:36:56Z |
| publishDate | 2016 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1051402022-10-01T16:06:14Z Three-dimensional manipulation of single cells using surface acoustic waves Guo, Feng Mao, Zhangming Chen, Yuchao Xie, Zhiwei Lata, James P. Li, Peng Ren, Liqiang Liu, Jiayang Yang, Jian Dao, Ming Suresh, Subra Huang, Tony Jun Massachusetts Institute of Technology. Center for Materials Science and Engineering Dao, Ming The ability of surface acoustic waves to trap and manipulate micrometer-scale particles and biological cells has led to many applications involving “acoustic tweezers” in biology, chemistry, engineering, and medicine. Here, we present 3D acoustic tweezers, which use surface acoustic waves to create 3D trapping nodes for the capture and manipulation of microparticles and cells along three mutually orthogonal axes. In this method, we use standing-wave phase shifts to move particles or cells in-plane, whereas the amplitude of acoustic vibrations is used to control particle motion along an orthogonal plane. We demonstrate, through controlled experiments guided by simulations, how acoustic vibrations result in micromanipulations in a microfluidic chamber by invoking physical principles that underlie the formation and regulation of complex, volumetric trapping nodes of particles and biological cells. We further show how 3D acoustic tweezers can be used to pick up, translate, and print single cells and cell assemblies to create 2D and 3D structures in a precise, noninvasive, label-free, and contact-free manner. National Institutes of Health (U.S.) (Grants 1R33EB019785-01 and 1 R01 GM112048-01A1) National Science Foundation (U.S.) National Institutes of Health (U.S.) (Pennsylvania State University. Center for Nanoscale Science. Grant DMR-0820404) National Institutes of Health (U.S.) (Grant U01HL114476) 2016-10-28T18:37:43Z 2016-10-28T18:37:43Z 2016-02 2015-11 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/105140 Guo, Feng et al. “Three-Dimensional Manipulation of Single Cells Using Surface Acoustic Waves.” Proceedings of the National Academy of Sciences 113.6 (2016): 1522–1527. en_US http://dx.doi.org/10.1073/pnas.1524813113 Proceedings of the National Academy of Sciences of the United States of America Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Guo, Feng Mao, Zhangming Chen, Yuchao Xie, Zhiwei Lata, James P. Li, Peng Ren, Liqiang Liu, Jiayang Yang, Jian Dao, Ming Suresh, Subra Huang, Tony Jun Three-dimensional manipulation of single cells using surface acoustic waves |
| title | Three-dimensional manipulation of single cells using surface acoustic waves |
| title_full | Three-dimensional manipulation of single cells using surface acoustic waves |
| title_fullStr | Three-dimensional manipulation of single cells using surface acoustic waves |
| title_full_unstemmed | Three-dimensional manipulation of single cells using surface acoustic waves |
| title_short | Three-dimensional manipulation of single cells using surface acoustic waves |
| title_sort | three dimensional manipulation of single cells using surface acoustic waves |
| url | http://hdl.handle.net/1721.1/105140 |
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