Loss of G[subscript α12/13] exacerbates apical area-dependence of actomyosin contractility
During development, coordinated cell shape changes alter tissue shape. In the Drosophila ventral furrow and other epithelia, apical constriction of hundreds of epithelial cells folds the tissue. Genes in the G[subscript α12/13] pathway coordinate collective apical constriction, but the mechanism of...
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| Format: | Article |
| Language: | en_US |
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American Society for Cell Biology
2016
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| Online Access: | http://hdl.handle.net/1721.1/105213 https://orcid.org/0000-0002-3283-3248 https://orcid.org/0000-0003-1338-494X https://orcid.org/0000-0001-8060-2607 |
| _version_ | 1826189169879154688 |
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| author | Xie, Shicong Mason, Frank M Martin, Adam C |
| author2 | Massachusetts Institute of Technology. Computational and Systems Biology Program |
| author_facet | Massachusetts Institute of Technology. Computational and Systems Biology Program Xie, Shicong Mason, Frank M Martin, Adam C |
| author_sort | Xie, Shicong |
| collection | MIT |
| description | During development, coordinated cell shape changes alter tissue shape. In the Drosophila ventral furrow and other epithelia, apical constriction of hundreds of epithelial cells folds the tissue. Genes in the G[subscript α12/13] pathway coordinate collective apical constriction, but the mechanism of coordination is poorly understood. Coupling live-cell imaging with a computational approach to identify contractile events, we discovered that differences in constriction behavior are biased by initial cell shape. Disrupting G[subscript α12/13] exacerbates this relationship. Larger apical area is associated with delayed initiation of contractile pulses, lower apical E-cadherin and F-actin levels, and aberrantly mobile Rho-Kinase structures. Our results suggest that loss of G[subscript α12/13] disrupts apical actin cortex organization and pulse initiation in a size-dependent manner. We propose that G[subscript α12/13] robustly organizes the apical cortex despite variation in apical area to ensure the timely initiation of contractile pulses in a tissue with heterogeneity in starting cell shape. |
| first_indexed | 2024-09-23T08:10:37Z |
| format | Article |
| id | mit-1721.1/105213 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:10:37Z |
| publishDate | 2016 |
| publisher | American Society for Cell Biology |
| record_format | dspace |
| spelling | mit-1721.1/1052132022-09-30T08:07:01Z Loss of G[subscript α12/13] exacerbates apical area-dependence of actomyosin contractility Loss of Gα12/13 exacerbates apical area dependence of actomyosin contractility Xie, Shicong Mason, Frank M Martin, Adam C Massachusetts Institute of Technology. Computational and Systems Biology Program Massachusetts Institute of Technology. Department of Biology Xie, Shicong Mason, Frank M Martin, Adam C During development, coordinated cell shape changes alter tissue shape. In the Drosophila ventral furrow and other epithelia, apical constriction of hundreds of epithelial cells folds the tissue. Genes in the G[subscript α12/13] pathway coordinate collective apical constriction, but the mechanism of coordination is poorly understood. Coupling live-cell imaging with a computational approach to identify contractile events, we discovered that differences in constriction behavior are biased by initial cell shape. Disrupting G[subscript α12/13] exacerbates this relationship. Larger apical area is associated with delayed initiation of contractile pulses, lower apical E-cadherin and F-actin levels, and aberrantly mobile Rho-Kinase structures. Our results suggest that loss of G[subscript α12/13] disrupts apical actin cortex organization and pulse initiation in a size-dependent manner. We propose that G[subscript α12/13] robustly organizes the apical cortex despite variation in apical area to ensure the timely initiation of contractile pulses in a tissue with heterogeneity in starting cell shape. 2016-11-04T20:05:57Z 2016-11-04T20:05:57Z 2016-08 2016-07 Article http://purl.org/eprint/type/JournalArticle 1059-1524 1939-4586 http://hdl.handle.net/1721.1/105213 Xie, S., F. M. Mason, and A. C. Martin. “Loss of G 12/13 Exacerbates Apical Area-Dependence of Actomyosin Contractility.” Molecular Biology of the Cell (2016): n. pag. © 2016 American Society for Cell Biology https://orcid.org/0000-0002-3283-3248 https://orcid.org/0000-0003-1338-494X https://orcid.org/0000-0001-8060-2607 en_US http://dx.doi.org/10.1091/mbc.E16-05-0305 Molecular Biology of the Cell Creative Commons Attribution-NonCommercial-ShareAlike 3.0 https://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Society for Cell Biology American Society for Cell Biology |
| spellingShingle | Xie, Shicong Mason, Frank M Martin, Adam C Loss of G[subscript α12/13] exacerbates apical area-dependence of actomyosin contractility |
| title | Loss of G[subscript α12/13] exacerbates apical area-dependence of actomyosin contractility |
| title_full | Loss of G[subscript α12/13] exacerbates apical area-dependence of actomyosin contractility |
| title_fullStr | Loss of G[subscript α12/13] exacerbates apical area-dependence of actomyosin contractility |
| title_full_unstemmed | Loss of G[subscript α12/13] exacerbates apical area-dependence of actomyosin contractility |
| title_short | Loss of G[subscript α12/13] exacerbates apical area-dependence of actomyosin contractility |
| title_sort | loss of g subscript α12 13 exacerbates apical area dependence of actomyosin contractility |
| url | http://hdl.handle.net/1721.1/105213 https://orcid.org/0000-0002-3283-3248 https://orcid.org/0000-0003-1338-494X https://orcid.org/0000-0001-8060-2607 |
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