Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug
Microparticles made from degradable polyhydroxyalkanoates of different chemical compositions a homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric and 3-hydroxyvaleric acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoic ac...
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| Format: | Article |
| Language: | English |
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Springer US
2016
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| Online Access: | http://hdl.handle.net/1721.1/105240 https://orcid.org/0000-0002-1015-1270 |
| _version_ | 1826200538753007616 |
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| author | Murueva, A. V. Shishatskaya, E. I. Kuzmina, A. M. Volova, T. G. Sinskey, Anthony J |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Murueva, A. V. Shishatskaya, E. I. Kuzmina, A. M. Volova, T. G. Sinskey, Anthony J |
| author_sort | Murueva, A. V. |
| collection | MIT |
| description | Microparticles made from degradable polyhydroxyalkanoates of different chemical compositions a homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric and 3-hydroxyvaleric acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoic acids (P3HB/3HHx) were prepared using the solvent evaporation technique, from double emulsions. The study addresses the influence of the chemical compositions on the size and ξ-potential of microparticles. P3HB microparticles loaded with doxorubicin have been prepared and investigated. Their average diameter and ξ-potential have been found to be dependent upon the level of loading (1, 5, and 10 % of the polymer mass). Investigation of the in vitro drug release behavior showed that the total drug released from the microparticle into the medium increased with mass concentration of the drug. In this study mouse fibroblast NIH 3T3 cells were cultivated on PHA microparticles, and results of using fluorescent DAPI DNA stain, and MTT assay showed that microparticles prepared from PHAs of different chemical compositions did not exhibit cytotoxicity to cells cultured on them and proved to be highly biocompatible. Cell attachment and proliferation on PHA microparticles were similar to those on polystyrene. The cytostatic drug encapsulated in P3HB/3HV microparticles has been proven to be effective against HeLa tumor cells. |
| first_indexed | 2024-09-23T11:37:58Z |
| format | Article |
| id | mit-1721.1/105240 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:37:58Z |
| publishDate | 2016 |
| publisher | Springer US |
| record_format | dspace |
| spelling | mit-1721.1/1052402022-10-01T04:56:07Z Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug Murueva, A. V. Shishatskaya, E. I. Kuzmina, A. M. Volova, T. G. Sinskey, Anthony J Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Engineering Systems Division Sinskey, Anthony J Microparticles made from degradable polyhydroxyalkanoates of different chemical compositions a homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric and 3-hydroxyvaleric acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoic acids (P3HB/3HHx) were prepared using the solvent evaporation technique, from double emulsions. The study addresses the influence of the chemical compositions on the size and ξ-potential of microparticles. P3HB microparticles loaded with doxorubicin have been prepared and investigated. Their average diameter and ξ-potential have been found to be dependent upon the level of loading (1, 5, and 10 % of the polymer mass). Investigation of the in vitro drug release behavior showed that the total drug released from the microparticle into the medium increased with mass concentration of the drug. In this study mouse fibroblast NIH 3T3 cells were cultivated on PHA microparticles, and results of using fluorescent DAPI DNA stain, and MTT assay showed that microparticles prepared from PHAs of different chemical compositions did not exhibit cytotoxicity to cells cultured on them and proved to be highly biocompatible. Cell attachment and proliferation on PHA microparticles were similar to those on polystyrene. The cytostatic drug encapsulated in P3HB/3HV microparticles has been proven to be effective against HeLa tumor cells. Russia (Federation) (Agreement 11.G34.31.0013) Program of the President of Russia for Young Doctors of Sciences (Grant MD-3112.2012.4) 2016-11-07T21:44:55Z 2016-11-07T21:44:55Z 2013-05 2012-07 2016-08-18T15:42:19Z Article http://purl.org/eprint/type/JournalArticle 0957-4530 1573-4838 http://hdl.handle.net/1721.1/105240 Murueva, A. V. et al. “Microparticles Prepared from Biodegradable Polyhydroxyalkanoates as Matrix for Encapsulation of Cytostatic Drug.” Journal of Materials Science: Materials in Medicine 24.8 (2013): 1905–1915. https://orcid.org/0000-0002-1015-1270 en http://dx.doi.org/10.1007/s10856-013-4941-2 Journal of Materials Science: Materials in Medicine Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ Springer Science+Business Media New York application/pdf Springer US Springer US |
| spellingShingle | Murueva, A. V. Shishatskaya, E. I. Kuzmina, A. M. Volova, T. G. Sinskey, Anthony J Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug |
| title | Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug |
| title_full | Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug |
| title_fullStr | Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug |
| title_full_unstemmed | Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug |
| title_short | Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug |
| title_sort | microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug |
| url | http://hdl.handle.net/1721.1/105240 https://orcid.org/0000-0002-1015-1270 |
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