Multiomic Analysis of the UV-Induced DNA Damage Response
In order to facilitate the identification of factors and pathways in the cellular response to UV-induced DNA damage, several descriptive proteomic screens and a functional genomics screen were performed in parallel. Numerous factors could be identified with high confidence when the screen results we...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Elsevier
2016
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| Online Access: | http://hdl.handle.net/1721.1/105271 https://orcid.org/0000-0003-4893-3152 |
| _version_ | 1811085706567614464 |
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| author | Boeing, Stefan Williamson, Laura Encheva, Vesela Gori, Ilaria Saunders, Rebecca E. Instrell, Rachael Rodriguez-Martinez, Marta Weems, Juston C. Kelly, Gavin P. Conaway, Joan W. Conaway, Ronald C. Stewart, Aengus Howell, Michael Snijders, Ambrosius P. Svejstrup, Jesper Q. Aygun, Ozan |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Boeing, Stefan Williamson, Laura Encheva, Vesela Gori, Ilaria Saunders, Rebecca E. Instrell, Rachael Rodriguez-Martinez, Marta Weems, Juston C. Kelly, Gavin P. Conaway, Joan W. Conaway, Ronald C. Stewart, Aengus Howell, Michael Snijders, Ambrosius P. Svejstrup, Jesper Q. Aygun, Ozan |
| author_sort | Boeing, Stefan |
| collection | MIT |
| description | In order to facilitate the identification of factors and pathways in the cellular response to UV-induced DNA damage, several descriptive proteomic screens and a functional genomics screen were performed in parallel. Numerous factors could be identified with high confidence when the screen results were superimposed and interpreted together, incorporating biological knowledge. A searchable database, bioLOGIC, which provides access to relevant information about a protein or process of interest, was established to host the results and facilitate data mining. Besides uncovering roles in the DNA damage response for numerous proteins and complexes, including Integrator, Cohesin, PHF3, ASC-1, SCAF4, SCAF8, and SCAF11, we uncovered a role for the poorly studied, melanoma-associated serine/threonine kinase 19 (STK19). Besides effectively uncovering relevant factors, the multiomic approach also provides a systems-wide overview of the diverse cellular processes connected to the transcription-related DNA damage response. |
| first_indexed | 2024-09-23T13:14:17Z |
| format | Article |
| id | mit-1721.1/105271 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:14:17Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1052712022-10-01T13:56:35Z Multiomic Analysis of the UV-Induced DNA Damage Response Boeing, Stefan Williamson, Laura Encheva, Vesela Gori, Ilaria Saunders, Rebecca E. Instrell, Rachael Rodriguez-Martinez, Marta Weems, Juston C. Kelly, Gavin P. Conaway, Joan W. Conaway, Ronald C. Stewart, Aengus Howell, Michael Snijders, Ambrosius P. Svejstrup, Jesper Q. Aygun, Ozan Massachusetts Institute of Technology. Department of Chemistry Aygun, Ozan In order to facilitate the identification of factors and pathways in the cellular response to UV-induced DNA damage, several descriptive proteomic screens and a functional genomics screen were performed in parallel. Numerous factors could be identified with high confidence when the screen results were superimposed and interpreted together, incorporating biological knowledge. A searchable database, bioLOGIC, which provides access to relevant information about a protein or process of interest, was established to host the results and facilitate data mining. Besides uncovering roles in the DNA damage response for numerous proteins and complexes, including Integrator, Cohesin, PHF3, ASC-1, SCAF4, SCAF8, and SCAF11, we uncovered a role for the poorly studied, melanoma-associated serine/threonine kinase 19 (STK19). Besides effectively uncovering relevant factors, the multiomic approach also provides a systems-wide overview of the diverse cellular processes connected to the transcription-related DNA damage response. Francis Crick Institute (grant FCI01) European Research Council Worldwide Cancer Research (Organization) 2016-11-09T20:16:30Z 2016-11-09T20:16:30Z 2016-05 2016-02 Article http://purl.org/eprint/type/JournalArticle 22111247 http://hdl.handle.net/1721.1/105271 Boeing, Stefan, Laura Williamson, Vesela Encheva, Ilaria Gori, Rebecca E. Saunders, Rachael Instrell, Ozan Aygün, et al. “Multiomic Analysis of the UV-Induced DNA Damage Response.” Cell Reports 15, no. 7 (May 2016): 1597–1610. https://orcid.org/0000-0003-4893-3152 en_US http://dx.doi.org/10.1016/j.celrep.2016.04.047 Cell Reports Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Elsevier Cell Reports |
| spellingShingle | Boeing, Stefan Williamson, Laura Encheva, Vesela Gori, Ilaria Saunders, Rebecca E. Instrell, Rachael Rodriguez-Martinez, Marta Weems, Juston C. Kelly, Gavin P. Conaway, Joan W. Conaway, Ronald C. Stewart, Aengus Howell, Michael Snijders, Ambrosius P. Svejstrup, Jesper Q. Aygun, Ozan Multiomic Analysis of the UV-Induced DNA Damage Response |
| title | Multiomic Analysis of the UV-Induced DNA Damage Response |
| title_full | Multiomic Analysis of the UV-Induced DNA Damage Response |
| title_fullStr | Multiomic Analysis of the UV-Induced DNA Damage Response |
| title_full_unstemmed | Multiomic Analysis of the UV-Induced DNA Damage Response |
| title_short | Multiomic Analysis of the UV-Induced DNA Damage Response |
| title_sort | multiomic analysis of the uv induced dna damage response |
| url | http://hdl.handle.net/1721.1/105271 https://orcid.org/0000-0003-4893-3152 |
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