Titanium Coating of the Boston Keratoprosthesis
Purpose: We tested the feasibility of using titanium to enhance adhesion of the Boston Keratoprosthesis (B-KPro), ultimately to decrease the risk of implant-associated complications. Methods: Cylindrical rods were made of poly(methyl methacrylate) (PMMA), PMMA coated with titanium dioxide (TiO[subsc...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Association for Research in Vision and Ophthalmology
2016
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| Online Access: | http://hdl.handle.net/1721.1/105353 |
| _version_ | 1826213879669063680 |
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| author | Jeong, Kyung Jae Kolovou, Paraskevi Evi Chodosh, James Dohlman, Claes H. Kohane, Daniel S. Chiang, Homer H. Salvador Culla, Borja |
| author2 | Koch Institute for Integrative Cancer Research at MIT |
| author_facet | Koch Institute for Integrative Cancer Research at MIT Jeong, Kyung Jae Kolovou, Paraskevi Evi Chodosh, James Dohlman, Claes H. Kohane, Daniel S. Chiang, Homer H. Salvador Culla, Borja |
| author_sort | Jeong, Kyung Jae |
| collection | MIT |
| description | Purpose: We tested the feasibility of using titanium to enhance adhesion of the Boston Keratoprosthesis (B-KPro), ultimately to decrease the risk of implant-associated complications. Methods: Cylindrical rods were made of poly(methyl methacrylate) (PMMA), PMMA coated with titanium dioxide (TiO[subscript 2]) over a layer of polydopamine (PMMA[subscript TiO2]), smooth (Ti) and sandblasted (Ti[subscript SB]) titanium, and titanium treated with oxygen plasma (Ti[subscript ox] and Ti[subscript SBox]). Topography and surface chemistry were analyzed by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). Adhesion force between rods and porcine corneas was measured ex vivo. Titanium sleeves, smooth and sandblasted, were inserted around the stem of the B-KPro and implanted in rabbits. Tissue adhesion to the stem was assessed and compared to an unmodified B-Kpro after 1 month. Results: X-ray photoelectron spectroscopy demonstrated successful deposition of TiO[subscript 2] on polydopamine-coated PMMA. Oxygen plasma treatment did not change the
XPS spectra of titanium rods (Ti and Ti[subscript SB]), although it increased their hydrophilicity. The materials did not show cell toxicity. After 14 days of incubation, PMMA[subscript TiO2], smooth titanium treated with oxygen plasma (Ti[subscript ox]), and sandblasted titanium rods (Ti[subscript SB], Ti[subscript SBox]) showed significantly higher adhesion forces than PMMA ex vivo. In vivo, the use of a Ti[subscript SB] sleeve around the stem of the B-KPro induced a significant increase in tissue adhesion compared to a Ti sleeve or bare PMMA. Conclusions: Sandblasted titanium sleeves greatly enhanced adherence of the B-KPro to the rabbit cornea. This approach may improve adhesion with the donor cornea in humans as well. Translational Relevance: This approach may improve adhesion with donor corneas in humans. |
| first_indexed | 2024-09-23T15:56:18Z |
| format | Article |
| id | mit-1721.1/105353 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:56:18Z |
| publishDate | 2016 |
| publisher | Association for Research in Vision and Ophthalmology |
| record_format | dspace |
| spelling | mit-1721.1/1053532022-10-02T05:12:28Z Titanium Coating of the Boston Keratoprosthesis Jeong, Kyung Jae Kolovou, Paraskevi Evi Chodosh, James Dohlman, Claes H. Kohane, Daniel S. Chiang, Homer H. Salvador Culla, Borja Koch Institute for Integrative Cancer Research at MIT Chiang, Homer H. Salvador Culla, Borja Purpose: We tested the feasibility of using titanium to enhance adhesion of the Boston Keratoprosthesis (B-KPro), ultimately to decrease the risk of implant-associated complications. Methods: Cylindrical rods were made of poly(methyl methacrylate) (PMMA), PMMA coated with titanium dioxide (TiO[subscript 2]) over a layer of polydopamine (PMMA[subscript TiO2]), smooth (Ti) and sandblasted (Ti[subscript SB]) titanium, and titanium treated with oxygen plasma (Ti[subscript ox] and Ti[subscript SBox]). Topography and surface chemistry were analyzed by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). Adhesion force between rods and porcine corneas was measured ex vivo. Titanium sleeves, smooth and sandblasted, were inserted around the stem of the B-KPro and implanted in rabbits. Tissue adhesion to the stem was assessed and compared to an unmodified B-Kpro after 1 month. Results: X-ray photoelectron spectroscopy demonstrated successful deposition of TiO[subscript 2] on polydopamine-coated PMMA. Oxygen plasma treatment did not change the XPS spectra of titanium rods (Ti and Ti[subscript SB]), although it increased their hydrophilicity. The materials did not show cell toxicity. After 14 days of incubation, PMMA[subscript TiO2], smooth titanium treated with oxygen plasma (Ti[subscript ox]), and sandblasted titanium rods (Ti[subscript SB], Ti[subscript SBox]) showed significantly higher adhesion forces than PMMA ex vivo. In vivo, the use of a Ti[subscript SB] sleeve around the stem of the B-KPro induced a significant increase in tissue adhesion compared to a Ti sleeve or bare PMMA. Conclusions: Sandblasted titanium sleeves greatly enhanced adherence of the B-KPro to the rabbit cornea. This approach may improve adhesion with the donor cornea in humans as well. Translational Relevance: This approach may improve adhesion with donor corneas in humans. Massachusetts Eye and Ear Infirmary. Keratoprosthesis Fund 2016-11-17T23:31:43Z 2016-11-17T23:31:43Z 2016-04 2015-09 Article http://purl.org/eprint/type/JournalArticle 2164-2591 http://hdl.handle.net/1721.1/105353 Salvador-Culla, Borja et al. “Titanium Coating of the Boston Keratoprosthesis.” Translational Vision Science & Technology 5.2 (2016): 17. © 2015 Association for Research in Vision and Ophthalmology en_US http://dx.doi.org/10.1167/tvst.5.2.17 Translational Vision Science & Technology Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Association for Research in Vision and Ophthalmology Association for Research in Vision and Ophthalmology (ARVO) |
| spellingShingle | Jeong, Kyung Jae Kolovou, Paraskevi Evi Chodosh, James Dohlman, Claes H. Kohane, Daniel S. Chiang, Homer H. Salvador Culla, Borja Titanium Coating of the Boston Keratoprosthesis |
| title | Titanium Coating of the Boston Keratoprosthesis |
| title_full | Titanium Coating of the Boston Keratoprosthesis |
| title_fullStr | Titanium Coating of the Boston Keratoprosthesis |
| title_full_unstemmed | Titanium Coating of the Boston Keratoprosthesis |
| title_short | Titanium Coating of the Boston Keratoprosthesis |
| title_sort | titanium coating of the boston keratoprosthesis |
| url | http://hdl.handle.net/1721.1/105353 |
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