Architectural transitions in Vibrio cholerae biofilms at single-cell resolution
Many bacterial species colonize surfaces and form dense 3D structures, known as biofilms, which are highly tolerant to antibiotics and constitute one of the major forms of bacterial biomass on Earth. Bacterial biofilms display remarkable changes during their development from initial attachment to ma...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2016
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| Online Access: | http://hdl.handle.net/1721.1/105382 https://orcid.org/0000-0001-8865-2369 |
| _version_ | 1826205637310152704 |
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| author | Drescher, Knut Nadell, Carey D. van Teeffelen, Sven Grnja, Ivan Wingreen, Ned S. Stone, Howard A. Bassler, Bonnie L. Dunkel, Joern |
| author2 | Massachusetts Institute of Technology. Department of Mathematics |
| author_facet | Massachusetts Institute of Technology. Department of Mathematics Drescher, Knut Nadell, Carey D. van Teeffelen, Sven Grnja, Ivan Wingreen, Ned S. Stone, Howard A. Bassler, Bonnie L. Dunkel, Joern |
| author_sort | Drescher, Knut |
| collection | MIT |
| description | Many bacterial species colonize surfaces and form dense 3D structures, known as biofilms, which are highly tolerant to antibiotics and constitute one of the major forms of bacterial biomass on Earth. Bacterial biofilms display remarkable changes during their development from initial attachment to maturity, yet the cellular architecture that gives rise to collective biofilm morphology during growth is largely unknown. Here, we use high-resolution optical microscopy to image all individual cells in Vibrio cholerae biofilms at different stages of development, including colonies that range in size from 2 to 4,500 cells. From these data, we extracted the precise 3D cellular arrangements, cell shapes, sizes, and global morphological features during biofilm growth on submerged glass substrates under flow. We discovered several critical transitions of the internal and external biofilm architectures that separate the major phases of V. cholerae biofilm growth. Optical imaging of biofilms with single-cell resolution provides a new window into biofilm formation that will prove invaluable to understanding the mechanics underlying biofilm development. |
| first_indexed | 2024-09-23T13:16:15Z |
| format | Article |
| id | mit-1721.1/105382 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:16:15Z |
| publishDate | 2016 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1053822022-09-28T13:04:04Z Architectural transitions in Vibrio cholerae biofilms at single-cell resolution Drescher, Knut Nadell, Carey D. van Teeffelen, Sven Grnja, Ivan Wingreen, Ned S. Stone, Howard A. Bassler, Bonnie L. Dunkel, Joern Massachusetts Institute of Technology. Department of Mathematics Dunkel, Joern Many bacterial species colonize surfaces and form dense 3D structures, known as biofilms, which are highly tolerant to antibiotics and constitute one of the major forms of bacterial biomass on Earth. Bacterial biofilms display remarkable changes during their development from initial attachment to maturity, yet the cellular architecture that gives rise to collective biofilm morphology during growth is largely unknown. Here, we use high-resolution optical microscopy to image all individual cells in Vibrio cholerae biofilms at different stages of development, including colonies that range in size from 2 to 4,500 cells. From these data, we extracted the precise 3D cellular arrangements, cell shapes, sizes, and global morphological features during biofilm growth on submerged glass substrates under flow. We discovered several critical transitions of the internal and external biofilm architectures that separate the major phases of V. cholerae biofilm growth. Optical imaging of biofilms with single-cell resolution provides a new window into biofilm formation that will prove invaluable to understanding the mechanics underlying biofilm development. Alfred P. Sloan Foundation 2016-11-21T18:34:59Z 2016-11-21T18:34:59Z 2016-04 2015-12 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/105382 Drescher, Knut et al. “Architectural Transitions in Vibrio Cholerae Biofilms at Single-Cell Resolution.” Proceedings of the National Academy of Sciences 113.14 (2016): E2066–E2072. © 2016 by the National Academy of Sciences https://orcid.org/0000-0001-8865-2369 en_US http://dx.doi.org/10.1073/pnas.1601702113 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Drescher, Knut Nadell, Carey D. van Teeffelen, Sven Grnja, Ivan Wingreen, Ned S. Stone, Howard A. Bassler, Bonnie L. Dunkel, Joern Architectural transitions in Vibrio cholerae biofilms at single-cell resolution |
| title | Architectural transitions in Vibrio cholerae biofilms at single-cell resolution |
| title_full | Architectural transitions in Vibrio cholerae biofilms at single-cell resolution |
| title_fullStr | Architectural transitions in Vibrio cholerae biofilms at single-cell resolution |
| title_full_unstemmed | Architectural transitions in Vibrio cholerae biofilms at single-cell resolution |
| title_short | Architectural transitions in Vibrio cholerae biofilms at single-cell resolution |
| title_sort | architectural transitions in vibrio cholerae biofilms at single cell resolution |
| url | http://hdl.handle.net/1721.1/105382 https://orcid.org/0000-0001-8865-2369 |
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