Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam
This study reports the genetic characterization of a highly pathogenic avian influenza virus subtype H5N1 isolated from a moribund domestic duck in central Vietnam during 2012. In the moribund duck’s flock, within 6 days after vaccination with a commercial H5N1 vaccine (Re-5) to 59-day-old birds, 12...
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| Format: | Article |
| Language: | English |
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Springer US
2016
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| Online Access: | http://hdl.handle.net/1721.1/105460 https://orcid.org/0000-0002-6747-7765 |
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| author | Ogawa, Haruko Imai, Kunitoshi Bui, Vuong Nghia Trinh, Dai Quang Nguyen, Tham Hong Thi Pham, Nga Thi Truong, Duc Anh Bui, Anh Ngoc Nguyen, Khong Viet Runstadler, Jonathan |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Ogawa, Haruko Imai, Kunitoshi Bui, Vuong Nghia Trinh, Dai Quang Nguyen, Tham Hong Thi Pham, Nga Thi Truong, Duc Anh Bui, Anh Ngoc Nguyen, Khong Viet Runstadler, Jonathan |
| author_sort | Ogawa, Haruko |
| collection | MIT |
| description | This study reports the genetic characterization of a highly pathogenic avian influenza virus subtype H5N1 isolated from a moribund domestic duck in central Vietnam during 2012. In the moribund duck’s flock, within 6 days after vaccination with a commercial H5N1 vaccine (Re-5) to 59-day-old birds, 120 out of 2,000 ducks died. Genetic analysis revealed a substantial number of mutations in the HA gene of the isolate in comparison with the vaccine strains, Re-1 and Re-5. Similar mutations were also found in selected Vietnamese H5N1 strains isolated since 2009. Mutations in the HA gene involved positions at antigenic sites associated with antibody binding and also neutralizing epitopes, with some of the mutations resulting in the modification of N-linked glycosylation of the HA. Those mutations may be related to the escape of virus from antibody binding and the infection of poultry, interpretations which may be confirmed through a reverse genetics approach. The virus also carried an amino acid substitution in the M2, which conferred a reduced susceptibility to amantadine, but no neuraminidase inhibitor resistance markers were found in the viral NA gene. Additional information including vaccination history in the farm and the surrounding area is needed to fully understand the background of this outbreak. Such understanding and expanded monitoring of the H5N1 influenza viruses circulating in Vietnam is an urgent need to provide updated information to improve effective vaccine strain selection and vaccination protocols, aiding disease control, and biosecurity to prevent H5N1 infection in both poultry and humans. |
| first_indexed | 2024-09-23T09:40:11Z |
| format | Article |
| id | mit-1721.1/105460 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:40:11Z |
| publishDate | 2016 |
| publisher | Springer US |
| record_format | dspace |
| spelling | mit-1721.1/1054602022-09-26T12:59:30Z Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam Ogawa, Haruko Imai, Kunitoshi Bui, Vuong Nghia Trinh, Dai Quang Nguyen, Tham Hong Thi Pham, Nga Thi Truong, Duc Anh Bui, Anh Ngoc Nguyen, Khong Viet Runstadler, Jonathan Massachusetts Institute of Technology. Department of Biological Engineering Runstadler, Jonathan This study reports the genetic characterization of a highly pathogenic avian influenza virus subtype H5N1 isolated from a moribund domestic duck in central Vietnam during 2012. In the moribund duck’s flock, within 6 days after vaccination with a commercial H5N1 vaccine (Re-5) to 59-day-old birds, 120 out of 2,000 ducks died. Genetic analysis revealed a substantial number of mutations in the HA gene of the isolate in comparison with the vaccine strains, Re-1 and Re-5. Similar mutations were also found in selected Vietnamese H5N1 strains isolated since 2009. Mutations in the HA gene involved positions at antigenic sites associated with antibody binding and also neutralizing epitopes, with some of the mutations resulting in the modification of N-linked glycosylation of the HA. Those mutations may be related to the escape of virus from antibody binding and the infection of poultry, interpretations which may be confirmed through a reverse genetics approach. The virus also carried an amino acid substitution in the M2, which conferred a reduced susceptibility to amantadine, but no neuraminidase inhibitor resistance markers were found in the viral NA gene. Additional information including vaccination history in the farm and the surrounding area is needed to fully understand the background of this outbreak. Such understanding and expanded monitoring of the H5N1 influenza viruses circulating in Vietnam is an urgent need to provide updated information to improve effective vaccine strain selection and vaccination protocols, aiding disease control, and biosecurity to prevent H5N1 infection in both poultry and humans. Japan Society for the Promotion of Science. Grant-in-Aid for the Bilateral Joint Projects Heiwa Nakajima Foundation National Institute of Allergy and Infectious Diseases (U.S.) (Contract HHSN2662007000010C) 2016-11-29T16:30:17Z 2016-11-29T16:30:17Z 2014-06 2014-02 2016-08-18T15:43:14Z Article http://purl.org/eprint/type/JournalArticle 0920-8569 1572-994X http://hdl.handle.net/1721.1/105460 Bui, Vuong Nghia et al. “Genetic Characterization of an H5N1 Avian Influenza Virus from a Vaccinated Duck Flock in Vietnam.” Virus Genes 49.2 (2014): 278–285. https://orcid.org/0000-0002-6747-7765 en http://dx.doi.org/10.1007/s11262-014-1089-1 Virus Genes Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ Springer Science+Business Media New York application/pdf Springer US Springer US |
| spellingShingle | Ogawa, Haruko Imai, Kunitoshi Bui, Vuong Nghia Trinh, Dai Quang Nguyen, Tham Hong Thi Pham, Nga Thi Truong, Duc Anh Bui, Anh Ngoc Nguyen, Khong Viet Runstadler, Jonathan Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam |
| title | Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam |
| title_full | Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam |
| title_fullStr | Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam |
| title_full_unstemmed | Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam |
| title_short | Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam |
| title_sort | genetic characterization of an h5n1 avian influenza virus from a vaccinated duck flock in vietnam |
| url | http://hdl.handle.net/1721.1/105460 https://orcid.org/0000-0002-6747-7765 |
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