Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment
Planarians are flatworms capable of regenerating any missing body region. This capacity is mediated by neoblasts, a proliferative cell population that contains pluripotent stem cells. Although population-based studies have revealed many neoblast characteristics, whether functionally distinct classes...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2016
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| Online Access: | http://hdl.handle.net/1721.1/105743 https://orcid.org/0000-0002-5569-333X |
| _version_ | 1826193502567923712 |
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| author | van Wolfswinkel, Josien C. Reddien, Peter Wagner, Daniel E. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology van Wolfswinkel, Josien C. Reddien, Peter Wagner, Daniel E. |
| author_sort | van Wolfswinkel, Josien C. |
| collection | MIT |
| description | Planarians are flatworms capable of regenerating any missing body region. This capacity is mediated by neoblasts, a proliferative cell population that contains pluripotent stem cells. Although population-based studies have revealed many neoblast characteristics, whether functionally distinct classes exist within this population is unclear. Here, we used high-dimensional single-cell transcriptional profiling from over a thousand individual neoblasts to directly compare gene expression fingerprints during homeostasis and regeneration. We identified two prominent neoblast classes that we named ζ (zeta) and σ (sigma). Zeta-neoblasts encompass
specified cells that give rise to an abundant postmitotic lineage including epidermal cells, and are
not required for regeneration. By contrast, sigma-neoblasts proliferate in response to injury,
possess broad lineage capacity, and can give rise to zeta-neoblasts. These findings present a new
view of planarian neoblasts, in which the population is comprised of two major and functionally
distinct cellular compartments. |
| first_indexed | 2024-09-23T09:40:11Z |
| format | Article |
| id | mit-1721.1/105743 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:40:11Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1057432022-09-30T16:06:05Z Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment van Wolfswinkel, Josien C. Reddien, Peter Wagner, Daniel E. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research van Wolfswinkel, Josien C. Wagner, Daniel Elger Reddien, Peter Planarians are flatworms capable of regenerating any missing body region. This capacity is mediated by neoblasts, a proliferative cell population that contains pluripotent stem cells. Although population-based studies have revealed many neoblast characteristics, whether functionally distinct classes exist within this population is unclear. Here, we used high-dimensional single-cell transcriptional profiling from over a thousand individual neoblasts to directly compare gene expression fingerprints during homeostasis and regeneration. We identified two prominent neoblast classes that we named ζ (zeta) and σ (sigma). Zeta-neoblasts encompass specified cells that give rise to an abundant postmitotic lineage including epidermal cells, and are not required for regeneration. By contrast, sigma-neoblasts proliferate in response to injury, possess broad lineage capacity, and can give rise to zeta-neoblasts. These findings present a new view of planarian neoblasts, in which the population is comprised of two major and functionally distinct cellular compartments. Human Frontier Science Program (Strasbourg, France) National Institutes of Health (U.S.) (Grant R01GM080639) 2016-12-07T20:00:38Z 2016-12-07T20:00:38Z 2014-07 2104-05 Article http://purl.org/eprint/type/JournalArticle 19345909 http://hdl.handle.net/1721.1/105743 van Wolfswinkel, Josien C., Daniel E. Wagner, and Peter W. Reddien. “Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment.” Cell Stem Cell 15.3 (2014): 326–339. https://orcid.org/0000-0002-5569-333X en_US http://dx.doi.org/10.1016/j.stem.2014.06.007 Cell Stem Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | van Wolfswinkel, Josien C. Reddien, Peter Wagner, Daniel E. Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment |
| title | Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment |
| title_full | Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment |
| title_fullStr | Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment |
| title_full_unstemmed | Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment |
| title_short | Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment |
| title_sort | single cell analysis reveals functionally distinct classes within the planarian stem cell compartment |
| url | http://hdl.handle.net/1721.1/105743 https://orcid.org/0000-0002-5569-333X |
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