Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A[subscript 1]A[subscript O] ATP synthase of Methanosarcina mazei Gö1
The interaction of the nucleotide-binding subunit B with subunit F is essential in coupling of ion pumping and ATP synthesis in A[subscript 1]A[subscript O] ATP synthases. Here we provide structural and thermodynamic insights on the nucleotide binding to the surface of subunits B and F of Methanosar...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer US
2017
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| Online Access: | http://hdl.handle.net/1721.1/106217 |
| _version_ | 1826190582765060096 |
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| author | Raghunathan, Devanathan Gayen, Shovanlal Kumar, Anil Hunke, Cornelia Grüber, Gerhard Verma, Chandra S. |
| author2 | Singapore-MIT Alliance in Research and Technology (SMART) |
| author_facet | Singapore-MIT Alliance in Research and Technology (SMART) Raghunathan, Devanathan Gayen, Shovanlal Kumar, Anil Hunke, Cornelia Grüber, Gerhard Verma, Chandra S. |
| author_sort | Raghunathan, Devanathan |
| collection | MIT |
| description | The interaction of the nucleotide-binding subunit B with subunit F is essential in coupling of ion pumping and ATP synthesis in A[subscript 1]A[subscript O] ATP synthases. Here we provide structural and thermodynamic insights on the nucleotide binding to the surface of subunits B and F of Methanosarcina mazei Gö1 A[subscript 1]A[subscript O] ATP synthase, which initiated migration to its final binding pocket via two transitional intermediates on the surface of subunit B. NMR- and fluorescence spectroscopy as well as ITC data combined with molecular dynamics simulations of the nucleotide bound subunit B and nucleotide bound B-F complex in explicit solvent, suggests that subunit F is critical for the migration to and eventual occupancy of the final binding site by the nucleotide of subunit B. Rotation of the C-terminus and conformational changes in subunit B are initiated upon binding with subunit F causing a perturbation that leads to the migration of ATP from the transition site 1 through an intermediate transition site 2 to the final binding site 3. This mechanism is elucidated on the basis of change in binding affinity for the nucleotide at the specific sites on subunit B upon complexation with subunit F. The change in enthalpy is further explained based on the fluctuating local environment around the binding sites. |
| first_indexed | 2024-09-23T08:42:33Z |
| format | Article |
| id | mit-1721.1/106217 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:42:33Z |
| publishDate | 2017 |
| publisher | Springer US |
| record_format | dspace |
| spelling | mit-1721.1/1062172022-09-23T14:01:57Z Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A[subscript 1]A[subscript O] ATP synthase of Methanosarcina mazei Gö1 Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A1AO ATP synthase of Methanosarcina mazei Gö1 Raghunathan, Devanathan Gayen, Shovanlal Kumar, Anil Hunke, Cornelia Grüber, Gerhard Verma, Chandra S. Singapore-MIT Alliance in Research and Technology (SMART) Raghunathan, Devanathan The interaction of the nucleotide-binding subunit B with subunit F is essential in coupling of ion pumping and ATP synthesis in A[subscript 1]A[subscript O] ATP synthases. Here we provide structural and thermodynamic insights on the nucleotide binding to the surface of subunits B and F of Methanosarcina mazei Gö1 A[subscript 1]A[subscript O] ATP synthase, which initiated migration to its final binding pocket via two transitional intermediates on the surface of subunit B. NMR- and fluorescence spectroscopy as well as ITC data combined with molecular dynamics simulations of the nucleotide bound subunit B and nucleotide bound B-F complex in explicit solvent, suggests that subunit F is critical for the migration to and eventual occupancy of the final binding site by the nucleotide of subunit B. Rotation of the C-terminus and conformational changes in subunit B are initiated upon binding with subunit F causing a perturbation that leads to the migration of ATP from the transition site 1 through an intermediate transition site 2 to the final binding site 3. This mechanism is elucidated on the basis of change in binding affinity for the nucleotide at the specific sites on subunit B upon complexation with subunit F. The change in enthalpy is further explained based on the fluctuating local environment around the binding sites. Nanyang Technological University Singapore. Agency for Science, Technology and Research (Singapore. Biomedical Research Council) 2017-01-05T21:14:05Z 2017-01-05T21:14:05Z 2012-02 2011-11 2016-08-18T15:42:59Z Article http://purl.org/eprint/type/JournalArticle 0145-479X 1573-6881 http://hdl.handle.net/1721.1/106217 Raghunathan, Devanathan et al. “Subunit F Modulates ATP Binding and Migration in the Nucleotide-Binding Subunit B of the A1AO ATP Synthase of Methanosarcina Mazei Gö1.” Journal of Bioenergetics and Biomembranes 44.1 (2012): 213–224. en http://dx.doi.org/10.1007/s10863-012-9410-y Journal of Bioenergetics and Biomembranes Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. Springer Science+Business Media, LLC application/pdf Springer US Springer US |
| spellingShingle | Raghunathan, Devanathan Gayen, Shovanlal Kumar, Anil Hunke, Cornelia Grüber, Gerhard Verma, Chandra S. Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A[subscript 1]A[subscript O] ATP synthase of Methanosarcina mazei Gö1 |
| title | Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A[subscript 1]A[subscript O] ATP synthase of Methanosarcina mazei Gö1 |
| title_full | Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A[subscript 1]A[subscript O] ATP synthase of Methanosarcina mazei Gö1 |
| title_fullStr | Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A[subscript 1]A[subscript O] ATP synthase of Methanosarcina mazei Gö1 |
| title_full_unstemmed | Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A[subscript 1]A[subscript O] ATP synthase of Methanosarcina mazei Gö1 |
| title_short | Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A[subscript 1]A[subscript O] ATP synthase of Methanosarcina mazei Gö1 |
| title_sort | subunit f modulates atp binding and migration in the nucleotide binding subunit b of the a subscript 1 a subscript o atp synthase of methanosarcina mazei go1 |
| url | http://hdl.handle.net/1721.1/106217 |
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