Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice
Recently, low-level laser (light) therapy has been used to increase muscle performance in intense exercises. However, there is a lack of understanding of the time response of muscles to light therapy. The first purpose of this study was to determine the time response for light-emitting diode therapy...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer London
2017
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| Online Access: | http://hdl.handle.net/1721.1/106485 |
| _version_ | 1826213342674419712 |
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| author | Ferraresi, Cleber Huang, Ying-Ying de Sousa, Marcelo Victor Pires Bagnato, Vanderlei Salvador Parizotto, Nivaldo Antonio Hamblin, Michael R. |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Ferraresi, Cleber Huang, Ying-Ying de Sousa, Marcelo Victor Pires Bagnato, Vanderlei Salvador Parizotto, Nivaldo Antonio Hamblin, Michael R. |
| author_sort | Ferraresi, Cleber |
| collection | MIT |
| description | Recently, low-level laser (light) therapy has been used to increase muscle performance in intense exercises. However, there is a lack of understanding of the time response of muscles to light therapy. The first purpose of this study was to determine the time response for light-emitting diode therapy (LEDT)-mediated increase in adenosine triphosphate (ATP) in the soleus and gastrocnemius muscles in mice. Second purpose was to test whether LEDT can increase the resistance of muscles to fatigue during intense exercise. Fifty male Balb/c mice were randomly allocated into two equal groups: LEDT-ATP and LEDT-fatigue. Both groups were subdivided into five equal subgroups: LEDT-sham, LEDT-5 min, LEDT-3 h, LEDT-6 h, and LEDT-24 h. Each subgroup was analyzed for muscle ATP content or fatigue at specified time after LEDT. The fatigue test was performed by mice repeatedly climbing an inclined ladder bearing a load of 150 % of body weight until exhaustion. LEDT used a cluster of LEDs with 20 red (630 ± 10 nm, 25 mW) and 20 infrared (850 ± 20 nm, 50 mW) delivering 80 mW/cm[superscript 2] for 90 s (7.2 J/cm[superscript 2]) applied to legs, gluteus, and lower back muscles. LEDT-6 h was the subgroup with the highest ATP content in soleus and gastrocnemius compared to all subgroups (P < 0.001). In addition, mice in LEDT-6 h group performed more repetitions in the fatigue test (P < 0.001) compared to all subgroups: LEDT-sham and LEDT-5 min (~600 %), LEDT-3 h (~200 %), and LEDT-24 h (~300 %). A high correlation between the fatigue test repetitions and the ATP content in soleus (r = 0.84) and gastrocnemius (r = 0.94) muscles was observed. LEDT increased ATP content in muscles and fatigue resistance in mice with a peak at 6 h. Although the time response in mice and humans is not the same, athletes might consider applying LEDT at 6 h before competition. |
| first_indexed | 2024-09-23T15:47:33Z |
| format | Article |
| id | mit-1721.1/106485 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:47:33Z |
| publishDate | 2017 |
| publisher | Springer London |
| record_format | dspace |
| spelling | mit-1721.1/1064852022-09-29T16:11:54Z Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice Ferraresi, Cleber Huang, Ying-Ying de Sousa, Marcelo Victor Pires Bagnato, Vanderlei Salvador Parizotto, Nivaldo Antonio Hamblin, Michael R. Massachusetts Institute of Technology. Institute for Medical Engineering & Science Harvard University--MIT Division of Health Sciences and Technology Hamblin, Michael R Recently, low-level laser (light) therapy has been used to increase muscle performance in intense exercises. However, there is a lack of understanding of the time response of muscles to light therapy. The first purpose of this study was to determine the time response for light-emitting diode therapy (LEDT)-mediated increase in adenosine triphosphate (ATP) in the soleus and gastrocnemius muscles in mice. Second purpose was to test whether LEDT can increase the resistance of muscles to fatigue during intense exercise. Fifty male Balb/c mice were randomly allocated into two equal groups: LEDT-ATP and LEDT-fatigue. Both groups were subdivided into five equal subgroups: LEDT-sham, LEDT-5 min, LEDT-3 h, LEDT-6 h, and LEDT-24 h. Each subgroup was analyzed for muscle ATP content or fatigue at specified time after LEDT. The fatigue test was performed by mice repeatedly climbing an inclined ladder bearing a load of 150 % of body weight until exhaustion. LEDT used a cluster of LEDs with 20 red (630 ± 10 nm, 25 mW) and 20 infrared (850 ± 20 nm, 50 mW) delivering 80 mW/cm[superscript 2] for 90 s (7.2 J/cm[superscript 2]) applied to legs, gluteus, and lower back muscles. LEDT-6 h was the subgroup with the highest ATP content in soleus and gastrocnemius compared to all subgroups (P < 0.001). In addition, mice in LEDT-6 h group performed more repetitions in the fatigue test (P < 0.001) compared to all subgroups: LEDT-sham and LEDT-5 min (~600 %), LEDT-3 h (~200 %), and LEDT-24 h (~300 %). A high correlation between the fatigue test repetitions and the ATP content in soleus (r = 0.84) and gastrocnemius (r = 0.94) muscles was observed. LEDT increased ATP content in muscles and fatigue resistance in mice with a peak at 6 h. Although the time response in mice and humans is not the same, athletes might consider applying LEDT at 6 h before competition. National Institutes of Health (U.S.) (grant R01AI050875) 2017-01-13T20:49:35Z 2017-01-13T20:49:35Z 2015-02 2014-11 2016-08-18T15:21:10Z Article http://purl.org/eprint/type/JournalArticle 0268-8921 1435-604X http://hdl.handle.net/1721.1/106485 Ferraresi, Cleber et al. “Time Response of Increases in ATP and Muscle Resistance to Fatigue after Low-Level Laser (Light) Therapy (LLLT) in Mice.” Lasers in Medical Science 30.4 (2015): 1259–1267. en http://dx.doi.org/10.1007/s10103-015-1723-8 Lasers in Medical Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. Springer-Verlag London application/pdf Springer London Springer London |
| spellingShingle | Ferraresi, Cleber Huang, Ying-Ying de Sousa, Marcelo Victor Pires Bagnato, Vanderlei Salvador Parizotto, Nivaldo Antonio Hamblin, Michael R. Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice |
| title | Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice |
| title_full | Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice |
| title_fullStr | Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice |
| title_full_unstemmed | Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice |
| title_short | Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice |
| title_sort | time response of increases in atp and muscle resistance to fatigue after low level laser light therapy lllt in mice |
| url | http://hdl.handle.net/1721.1/106485 |
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