Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion
Muscle regeneration is a complex phenomenon, involving replacement of damaged fibers by new muscle fibers. During this process, there is a tendency to form scar tissue or fibrosis by deposition of collagen that could be detrimental to muscle function. New therapies that could regulate fibrosis and f...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Springer-Verlag
2017
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| Online Access: | http://hdl.handle.net/1721.1/106488 |
| _version_ | 1811095580962717696 |
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| author | Assis, Lívia Moretti, Ana Iochabel Soares Abrahão, Thalita Balsamo de Souza, Heraldo Possolo Parizotto, Nivaldo Antonio Hamblin, Michael R. |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Assis, Lívia Moretti, Ana Iochabel Soares Abrahão, Thalita Balsamo de Souza, Heraldo Possolo Parizotto, Nivaldo Antonio Hamblin, Michael R. |
| author_sort | Assis, Lívia |
| collection | MIT |
| description | Muscle regeneration is a complex phenomenon, involving replacement of damaged fibers by new muscle fibers. During this process, there is a tendency to form scar tissue or fibrosis by deposition of collagen that could be detrimental to muscle function. New therapies that could regulate fibrosis and favor muscle regeneration would be important for physical therapy. Low-level laser therapy (LLLT) has been studied for clinical treatment of skeletal muscle injuries and disorders, even though the molecular and cellular mechanisms have not yet been clarified. The aim of this study was to evaluate the effects of LLLT on molecular markers involved in muscle fibrosis and regeneration after cryolesion of the tibialis anterior (TA) muscle in rats. Sixty Wistar rats were randomly divided into three groups: control, injured TA muscle without LLLT, injured TA muscle treated with LLLT. The injured region was irradiated daily for four consecutive days, starting immediately after the lesion using an AlGaAs laser (808 nm, 30 mW, 180 J/cm[superscript 2]; 3.8 W/cm[superscript 2], 1.4 J). The animals were sacrificed on the fourth day after injury. LLLT significantly reduced the lesion percentage area in the injured muscle (p < 0.05), increased mRNA levels of the transcription factors MyoD and myogenin (p < 0.01) and the pro-angiogenic vascular endothelial growth factor (p < 0.01). Moreover, LLLT decreased the expression of the profibrotic transforming growth factor TGF-β mRNA (p < 0.01) and reduced type I collagen deposition (p < 0.01). These results suggest that LLLT could be an effective therapeutic approach for promoting skeletal muscle regeneration while preventing tissue fibrosis after muscle injury. |
| first_indexed | 2024-09-23T16:19:47Z |
| format | Article |
| id | mit-1721.1/106488 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:19:47Z |
| publishDate | 2017 |
| publisher | Springer-Verlag |
| record_format | dspace |
| spelling | mit-1721.1/1064882022-09-29T19:35:08Z Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion Assis, Lívia Moretti, Ana Iochabel Soares Abrahão, Thalita Balsamo de Souza, Heraldo Possolo Parizotto, Nivaldo Antonio Hamblin, Michael R. Massachusetts Institute of Technology. Institute for Medical Engineering & Science Harvard University--MIT Division of Health Sciences and Technology Hamblin, Michael R Muscle regeneration is a complex phenomenon, involving replacement of damaged fibers by new muscle fibers. During this process, there is a tendency to form scar tissue or fibrosis by deposition of collagen that could be detrimental to muscle function. New therapies that could regulate fibrosis and favor muscle regeneration would be important for physical therapy. Low-level laser therapy (LLLT) has been studied for clinical treatment of skeletal muscle injuries and disorders, even though the molecular and cellular mechanisms have not yet been clarified. The aim of this study was to evaluate the effects of LLLT on molecular markers involved in muscle fibrosis and regeneration after cryolesion of the tibialis anterior (TA) muscle in rats. Sixty Wistar rats were randomly divided into three groups: control, injured TA muscle without LLLT, injured TA muscle treated with LLLT. The injured region was irradiated daily for four consecutive days, starting immediately after the lesion using an AlGaAs laser (808 nm, 30 mW, 180 J/cm[superscript 2]; 3.8 W/cm[superscript 2], 1.4 J). The animals were sacrificed on the fourth day after injury. LLLT significantly reduced the lesion percentage area in the injured muscle (p < 0.05), increased mRNA levels of the transcription factors MyoD and myogenin (p < 0.01) and the pro-angiogenic vascular endothelial growth factor (p < 0.01). Moreover, LLLT decreased the expression of the profibrotic transforming growth factor TGF-β mRNA (p < 0.01) and reduced type I collagen deposition (p < 0.01). These results suggest that LLLT could be an effective therapeutic approach for promoting skeletal muscle regeneration while preventing tissue fibrosis after muscle injury. National Institutes of Health (U.S.) (grant R01AI050875) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Conselho Nacional de Pesquisas (Brazil) Fundação de Amparo à Pesquisa do Estado de São Paulo 2017-01-13T21:12:59Z 2017-01-13T21:12:59Z 2012-08 2012-02 2016-08-18T15:21:06Z Article http://purl.org/eprint/type/JournalArticle 0268-8921 1435-604X http://hdl.handle.net/1721.1/106488 Assis, Lívia et al. “Low-Level Laser Therapy (808 Nm) Contributes to Muscle Regeneration and Prevents Fibrosis in Rat Tibialis Anterior Muscle after Cryolesion.” Lasers in Medical Science 28.3 (2013): 947–955. en http://dx.doi.org/10.1007/s10103-012-1183-3 Lasers in Medical Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. Springer-Verlag London Ltd application/pdf Springer-Verlag Springer-Verlag |
| spellingShingle | Assis, Lívia Moretti, Ana Iochabel Soares Abrahão, Thalita Balsamo de Souza, Heraldo Possolo Parizotto, Nivaldo Antonio Hamblin, Michael R. Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion |
| title | Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion |
| title_full | Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion |
| title_fullStr | Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion |
| title_full_unstemmed | Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion |
| title_short | Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion |
| title_sort | low level laser therapy 808 nm contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion |
| url | http://hdl.handle.net/1721.1/106488 |
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