Inferring transient particle transport dynamics in live cells
Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular transport. However, trajectory analysis procedures to infer complex transport dynamics involving stochastic switching between active transport and diffusive motion are lacking. We applied Bayesian model s...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/106511 https://orcid.org/0000-0001-8844-7170 https://orcid.org/0000-0003-4573-5206 https://orcid.org/0000-0002-3829-5612 https://orcid.org/0000-0002-6199-6855 |
| _version_ | 1811093800061239296 |
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| author | Park, Hye Yoon Katz, Zachary English, Brian P. Singer, Robert H. Monnier, Nilah Barry, Zachary Thomas Su, Kuan-Chung Dey, Arkajit Pan, Keyao Cheeseman, Iain M Bathe, Mark |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Park, Hye Yoon Katz, Zachary English, Brian P. Singer, Robert H. Monnier, Nilah Barry, Zachary Thomas Su, Kuan-Chung Dey, Arkajit Pan, Keyao Cheeseman, Iain M Bathe, Mark |
| author_sort | Park, Hye Yoon |
| collection | MIT |
| description | Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular transport. However, trajectory analysis procedures to infer complex transport dynamics involving stochastic switching between active transport and diffusive motion are lacking. We applied Bayesian model selection to hidden Markov modeling to infer transient transport states from trajectories of mRNA-protein complexes in live mouse hippocampal neurons and metaphase kinetochores in dividing human cells. The software is available at http://hmm-bayes.org/. |
| first_indexed | 2024-09-23T15:50:53Z |
| format | Article |
| id | mit-1721.1/106511 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:50:53Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1065112022-09-29T16:34:30Z Inferring transient particle transport dynamics in live cells Park, Hye Yoon Katz, Zachary English, Brian P. Singer, Robert H. Monnier, Nilah Barry, Zachary Thomas Su, Kuan-Chung Dey, Arkajit Pan, Keyao Cheeseman, Iain M Bathe, Mark Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Monnier, Nilah Barry, Zachary Thomas Su, Kuan-Chung Dey, Arkajit Pan, Keyao Cheeseman, Iain M Bathe, Mark Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular transport. However, trajectory analysis procedures to infer complex transport dynamics involving stochastic switching between active transport and diffusive motion are lacking. We applied Bayesian model selection to hidden Markov modeling to infer transient transport states from trajectories of mRNA-protein complexes in live mouse hippocampal neurons and metaphase kinetochores in dividing human cells. The software is available at http://hmm-bayes.org/. National Institutes of Health (U.S.) National Institute of Mental Health (U.S.) (Grant U01 MH106011) National Science Foundation (U.S.). Physics of Living Systems (Grant PHY 1305537) Leukemia & Lymphoma Society of America (Scholar Award) National Institute of General Medical Sciences (U.S.) (Grant GM088313) Austrian Science Fund (Schroedinger Fellowship) 2017-01-17T19:12:46Z 2017-01-17T19:12:46Z 2015-07 2015-01 Article http://purl.org/eprint/type/JournalArticle 1548-7091 1548-7105 http://hdl.handle.net/1721.1/106511 Monnier, Nilah et al. “Inferring Transient Particle Transport Dynamics in Live Cells.” Nature Methods 12.9 (2015): 838–840. https://orcid.org/0000-0001-8844-7170 https://orcid.org/0000-0003-4573-5206 https://orcid.org/0000-0002-3829-5612 https://orcid.org/0000-0002-6199-6855 en_US http://dx.doi.org/10.1038/nmeth.3483 Nature Methods Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Park, Hye Yoon Katz, Zachary English, Brian P. Singer, Robert H. Monnier, Nilah Barry, Zachary Thomas Su, Kuan-Chung Dey, Arkajit Pan, Keyao Cheeseman, Iain M Bathe, Mark Inferring transient particle transport dynamics in live cells |
| title | Inferring transient particle transport dynamics in live cells |
| title_full | Inferring transient particle transport dynamics in live cells |
| title_fullStr | Inferring transient particle transport dynamics in live cells |
| title_full_unstemmed | Inferring transient particle transport dynamics in live cells |
| title_short | Inferring transient particle transport dynamics in live cells |
| title_sort | inferring transient particle transport dynamics in live cells |
| url | http://hdl.handle.net/1721.1/106511 https://orcid.org/0000-0001-8844-7170 https://orcid.org/0000-0003-4573-5206 https://orcid.org/0000-0002-3829-5612 https://orcid.org/0000-0002-6199-6855 |
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