In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light
Purpose The poor tissue penetration of visible light has been a major barrier for optical imaging, photoactivatable conversions, and photodynamic therapy for in vivo targets with depths beyond 10 mm. In this report, as a proof-of-concept, we demonstrated that a positron emission tomography (PET)...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer-Verlag
2017
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| Online Access: | http://hdl.handle.net/1721.1/106641 |
| _version_ | 1826216656803725312 |
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| author | Moore, Anna V Ran, Chongzhao Zhang, Zhaoda Hooker, Jacob |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Moore, Anna V Ran, Chongzhao Zhang, Zhaoda Hooker, Jacob |
| author_sort | Moore, Anna V |
| collection | MIT |
| description | Purpose
The poor tissue penetration of visible light has been a major barrier for optical imaging, photoactivatable conversions, and photodynamic therapy for in vivo targets with depths beyond 10 mm. In this report, as a proof-of-concept, we demonstrated that a positron emission tomography (PET) radiotracer, 2-deoxy-2-[[superscript 18]F]fluoro-d-glucose ([superscript 18]FDG), could be used as an alternative light source for photoactivation.
Procedures
We utilized [superscript 18]FDG, which is a metabolic activity-based PET probe, as a source of light to photoactivate caged luciferin in a breast cancer animal model expressing luciferase.
Results
Bioluminescence produced from luciferin allowed for the real-time monitoring of Cherenkov radiation-promoted uncaging of the substrate.
Conclusion
The proposed method may provide a very important option for in vivo photoactivation, in particular for activation of photosensitizers for photodynamic therapy and eventually for combining radioisotope therapy and photodynamic therapy. |
| first_indexed | 2024-09-23T16:51:18Z |
| format | Article |
| id | mit-1721.1/106641 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:51:18Z |
| publishDate | 2017 |
| publisher | Springer-Verlag |
| record_format | dspace |
| spelling | mit-1721.1/1066412022-10-03T08:44:24Z In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light Moore, Anna V Ran, Chongzhao Zhang, Zhaoda Hooker, Jacob Massachusetts Institute of Technology. Institute for Medical Engineering & Science Martinos Imaging Center (McGovern Institute for Brain Research at MIT) Harvard University--MIT Division of Health Sciences and Technology McGovern Institute for Brain Research at MIT Moore, Anna V Ran, Chongzhao Zhang, Zhaoda Hooker, Jacob Purpose The poor tissue penetration of visible light has been a major barrier for optical imaging, photoactivatable conversions, and photodynamic therapy for in vivo targets with depths beyond 10 mm. In this report, as a proof-of-concept, we demonstrated that a positron emission tomography (PET) radiotracer, 2-deoxy-2-[[superscript 18]F]fluoro-d-glucose ([superscript 18]FDG), could be used as an alternative light source for photoactivation. Procedures We utilized [superscript 18]FDG, which is a metabolic activity-based PET probe, as a source of light to photoactivate caged luciferin in a breast cancer animal model expressing luciferase. Results Bioluminescence produced from luciferin allowed for the real-time monitoring of Cherenkov radiation-promoted uncaging of the substrate. Conclusion The proposed method may provide a very important option for in vivo photoactivation, in particular for activation of photosensitizers for photodynamic therapy and eventually for combining radioisotope therapy and photodynamic therapy. 2017-01-26T21:46:06Z 2017-01-26T21:46:06Z 2011-05 2016-08-18T15:45:05Z Article http://purl.org/eprint/type/JournalArticle 1536-1632 1860-2002 http://hdl.handle.net/1721.1/106641 Ran, Chongzhao et al. “In Vivo Photoactivation Without ‘Light’: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light.” Molecular Imaging and Biology 14.2 (2012): 156–162. en http://dx.doi.org/10.1007/s11307-011-0489-z Molecular Imaging and Biology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. Academy of Molecular Imaging and Society for Molecular Imaging application/pdf Springer-Verlag Springer-Verlag |
| spellingShingle | Moore, Anna V Ran, Chongzhao Zhang, Zhaoda Hooker, Jacob In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light |
| title | In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light |
| title_full | In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light |
| title_fullStr | In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light |
| title_full_unstemmed | In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light |
| title_short | In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light |
| title_sort | in vivo photoactivation without light use of cherenkov radiation to overcome the penetration limit of light |
| url | http://hdl.handle.net/1721.1/106641 |
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