Comparison of Cas9 activators in multiple species

Comparison of Cas9 activators in multiple species

Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several hu...

Full description

Bibliographic Details
Main Authors: Chavez, Alejandro, Tuttle, Marcelle, Pruitt, Benjamin W, Ewen-Campen, Ben, Chari, Raj, Ter-Ovanesyan, Dmitry, Haque, Sabina J, Cecchi, Ryan J, Kowal, Emma J K, Buchthal, Joanna, Housden, Benjamin E, Perrimon, Norbert, Church, George, Collins, James J.
Other Authors: Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Format: Article
Language:en_US
Published: Nature Publishing Group 2017
Online Access:http://hdl.handle.net/1721.1/106662
https://orcid.org/0000-0002-5560-8246
Description
Summary:Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression.

Similar Items