Comparison of Cas9 activators in multiple species
Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several hu...
| Main Authors: | , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/106662 https://orcid.org/0000-0002-5560-8246 |
| _version_ | 1826198911196332032 |
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| author | Chavez, Alejandro Tuttle, Marcelle Pruitt, Benjamin W Ewen-Campen, Ben Chari, Raj Ter-Ovanesyan, Dmitry Haque, Sabina J Cecchi, Ryan J Kowal, Emma J K Buchthal, Joanna Housden, Benjamin E Perrimon, Norbert Church, George Collins, James J. |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Chavez, Alejandro Tuttle, Marcelle Pruitt, Benjamin W Ewen-Campen, Ben Chari, Raj Ter-Ovanesyan, Dmitry Haque, Sabina J Cecchi, Ryan J Kowal, Emma J K Buchthal, Joanna Housden, Benjamin E Perrimon, Norbert Church, George Collins, James J. |
| author_sort | Chavez, Alejandro |
| collection | MIT |
| description | Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression. |
| first_indexed | 2024-09-23T11:11:53Z |
| format | Article |
| id | mit-1721.1/106662 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:11:53Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1066622022-10-01T01:58:22Z Comparison of Cas9 activators in multiple species Chavez, Alejandro Tuttle, Marcelle Pruitt, Benjamin W Ewen-Campen, Ben Chari, Raj Ter-Ovanesyan, Dmitry Haque, Sabina J Cecchi, Ryan J Kowal, Emma J K Buchthal, Joanna Housden, Benjamin E Perrimon, Norbert Church, George Collins, James J. Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Synthetic Biology Center Massachusetts Institute of Technology. Department of Biological Engineering Collins, James J. Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression. United States. Defense Threat Reduction Agency (Grant HDTRA1-14-1-0006) 2017-01-27T20:45:31Z 2017-01-27T20:45:31Z 2016-05 2016-01 Article http://purl.org/eprint/type/JournalArticle 1548-7091 1548-7105 http://hdl.handle.net/1721.1/106662 .Chavez, Alejandro et al. “Comparison of Cas9 Activators in Multiple Species.” Nature Methods 13.7 (2016): 563–567. https://orcid.org/0000-0002-5560-8246 en_US http://dx.doi.org/10.1038/nmeth.3871 Nature Methods Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Chavez, Alejandro Tuttle, Marcelle Pruitt, Benjamin W Ewen-Campen, Ben Chari, Raj Ter-Ovanesyan, Dmitry Haque, Sabina J Cecchi, Ryan J Kowal, Emma J K Buchthal, Joanna Housden, Benjamin E Perrimon, Norbert Church, George Collins, James J. Comparison of Cas9 activators in multiple species |
| title | Comparison of Cas9 activators in multiple species |
| title_full | Comparison of Cas9 activators in multiple species |
| title_fullStr | Comparison of Cas9 activators in multiple species |
| title_full_unstemmed | Comparison of Cas9 activators in multiple species |
| title_short | Comparison of Cas9 activators in multiple species |
| title_sort | comparison of cas9 activators in multiple species |
| url | http://hdl.handle.net/1721.1/106662 https://orcid.org/0000-0002-5560-8246 |
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