Identification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as a novel autophagy regulator by high content shRNA screening

Identification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as a novel autophagy regulator by high content shRNA screening

Deregulation of autophagy has been linked to multiple degenerative diseases and cancer, thus the identification of novel autophagy regulators for potential therapeutic intervention is important. To meet this need, we developed a high content image-based short hairpin RNA screen monitoring levels of...

Cur síos iomlán

Sonraí bibleagrafaíochta
Príomhchruthaitheoirí:	Strohecker, A M, Joshi, S, Abraham, R T, White, E, Possemato, Richard, Sabatini, David
Rannpháirtithe:	Massachusetts Institute of Technology. Department of Biology
Formáid:	Alt
Teanga:	en_US
Foilsithe / Cruthaithe:	Nature Publishing Group 2017
Rochtain ar líne:	http://hdl.handle.net/1721.1/106673 https://orcid.org/0000-0002-2401-0030 https://orcid.org/0000-0002-1446-7256

Míreanna comhchosúla

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Kinetic properties of bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from spinach leaves.
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de réir: Scott, P, et al.
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de réir: Kruger, N, et al.
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Physiological relevance of fructose 2,6-bisphosphate in the regulation of spinach leaf pyrophosphate:fructose 6-phosphate 1-phosphotransferase.
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de réir: Kruger, N, et al.
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Efficient shRNA-Mediated Inhibition of Gene Expression in Zebrafish
de réir: Sive, Hazel L., et al.
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Alternative approach to generate shRNA from cDNA
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Virtual screening, molecular docking, molecular dynamics, and MM-GBSA approaches identify prospective fructose-1,6-bisphosphatase inhibitors from pineapple for diabetes management
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INFLUENCE OF FRUCTOSE 2,6-BISPHOSPHATE LEVELS ON STARCH MOBILIZATION IN TRANSGENIC TOBACCO-LEAVES IN THE DARK
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Effect of recombinant cytokines on glycolysis and fructose 2,6-bisphosphate in rheumatoid synovial cells in vitro.
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Stable Interference by shRNA with pSUPER.retro Vectors and Lipofectamine
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Influence of Elevated Fructose-2,6-Bisphosphate Levels on Starch Mobilization in Transgenic Tobacco Leaves in the Dark.
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Synthesis of 2,6-Hexa-Tert-Butylterphenyl Derivatives, 2,6-(2,4,6-t-Bu₃C₆H₂)₂C₆H₃X, Where X = I, Li, OH, SH, N₃, or NH₂
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Intranasal delivery of shRNA to knockdown the 5HT-2A receptor enhances memory and alleviates anxiety
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Activation of pyrophosphate:fructose-6-phosphate 1-phosphotransferase by fructose 2,6-bisphosphate stimulates conversion of hexose phosphates to triose phosphates but does not influence accumulation of carbohydrates in phosphate-deficient tobacco cells.
de réir: Fernie, A, et al.
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Carbon metabolism in leaves of transgenic tobacco (Nicotiana tabacum L.) containing elevated fructose 2,6-bisphosphate levels.
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In vivo shRNA screen reveals PRMT5 as a key regulator in glioblastoma
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Photosynthetic carbon metabolism in leaves of transgenic tobacco (Nicotiana tabacum L.) containing decreased amounts of fructose 2,6-bisphosphate.
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