Artificially Engineered Protein Hydrogels Adapted from the Nucleoporin Nsp1 for Selective Biomolecular Transport
Nucleoporin-like polypeptide (NLP) hydrogels are developed by mimicking nucleoporins, proteins that form gel filters regulating transport into the nucleus. Using protein polymers of a minimal consensus repeat, the NLPs selectively enhance transport of cargo–carrier complexes similar to the natural n...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2017
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| Online Access: | http://hdl.handle.net/1721.1/106834 https://orcid.org/0000-0002-9476-6351 https://orcid.org/0000-0003-1646-2456 https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-7272-7140 |
| _version_ | 1811090697949806592 |
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| author | Kim, Minkyu Chen, Wesley George Ribbeck, Katharina Olsen, Bradley D Kang, Jeon Woong Glassman, Matthew J. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Kim, Minkyu Chen, Wesley George Ribbeck, Katharina Olsen, Bradley D Kang, Jeon Woong Glassman, Matthew J. |
| author_sort | Kim, Minkyu |
| collection | MIT |
| description | Nucleoporin-like polypeptide (NLP) hydrogels are developed by mimicking nucleoporins, proteins that form gel filters regulating transport into the nucleus. Using protein polymers of a minimal consensus repeat, the NLPs selectively enhance transport of cargo–carrier complexes similar to the natural nuclear pore system. The engineered protein gels additionally have tunable mechanical and transport properties and can be biosynthesized at high yield, making them promising materials for advanced separation technologies. |
| first_indexed | 2024-09-23T14:50:27Z |
| format | Article |
| id | mit-1721.1/106834 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:50:27Z |
| publishDate | 2017 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/1068342022-09-29T10:54:12Z Artificially Engineered Protein Hydrogels Adapted from the Nucleoporin Nsp1 for Selective Biomolecular Transport Kim, Minkyu Chen, Wesley George Ribbeck, Katharina Olsen, Bradley D Kang, Jeon Woong Glassman, Matthew J. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Laser Biomedical Research Center Kim, Minkyu Chen, Wesley George Glassman, Matthew James Ribbeck, Katharina Olsen, Bradley D Kang, Jeon Woong Nucleoporin-like polypeptide (NLP) hydrogels are developed by mimicking nucleoporins, proteins that form gel filters regulating transport into the nucleus. Using protein polymers of a minimal consensus repeat, the NLPs selectively enhance transport of cargo–carrier complexes similar to the natural nuclear pore system. The engineered protein gels additionally have tunable mechanical and transport properties and can be biosynthesized at high yield, making them promising materials for advanced separation technologies. United States. Defense Threat Reduction Agency (Grant HDTRA1-13-1-0038) National Institutes of Health (U.S.) (Grant 5-T32-GM008834) National Institute for Biomedical Imaging and Bioengineering (U.S.) (Grant P41EB015871-28) MIT Skoltech Initiative 2017-02-02T20:31:19Z 2017-02-02T20:31:19Z 2015-06 2015-04 Article http://purl.org/eprint/type/JournalArticle 0935-9648 1521-4095 http://hdl.handle.net/1721.1/106834 Kim, Minkyu et al. “Artificially Engineered Protein Hydrogels Adapted from the Nucleoporin Nsp1 for Selective Biomolecular Transport.” Advanced Materials 27.28 (2015): 4207–4212. https://orcid.org/0000-0002-9476-6351 https://orcid.org/0000-0003-1646-2456 https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-7272-7140 en_US http://dx.doi.org/10.1002/adma.201500752 Advanced Materials Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Kim, Minkyu Chen, Wesley George Ribbeck, Katharina Olsen, Bradley D Kang, Jeon Woong Glassman, Matthew J. Artificially Engineered Protein Hydrogels Adapted from the Nucleoporin Nsp1 for Selective Biomolecular Transport |
| title | Artificially Engineered Protein Hydrogels Adapted from the Nucleoporin Nsp1 for Selective Biomolecular Transport |
| title_full | Artificially Engineered Protein Hydrogels Adapted from the Nucleoporin Nsp1 for Selective Biomolecular Transport |
| title_fullStr | Artificially Engineered Protein Hydrogels Adapted from the Nucleoporin Nsp1 for Selective Biomolecular Transport |
| title_full_unstemmed | Artificially Engineered Protein Hydrogels Adapted from the Nucleoporin Nsp1 for Selective Biomolecular Transport |
| title_short | Artificially Engineered Protein Hydrogels Adapted from the Nucleoporin Nsp1 for Selective Biomolecular Transport |
| title_sort | artificially engineered protein hydrogels adapted from the nucleoporin nsp1 for selective biomolecular transport |
| url | http://hdl.handle.net/1721.1/106834 https://orcid.org/0000-0002-9476-6351 https://orcid.org/0000-0003-1646-2456 https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-7272-7140 |
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