A measure of genetic diversity of goldenseal (Hydrastis canadensis L.) by RAPD analysis
Goldenseal (Hydrastis canadensis L.) is a medicinal plant valued for the treatment of sore eyes and mouths. Although cultivation of the plant has helped meet growing demand, goldenseal is still considered a threatened or endangered species throughout much of its range in North America. In an effort...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer Netherlands
2017
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| Online Access: | http://hdl.handle.net/1721.1/106863 |
| _version_ | 1826189905499258880 |
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| author | Inoue, Maiko Frary, Amy Craker, Lyle E. Kelley, Kerry |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Inoue, Maiko Frary, Amy Craker, Lyle E. Kelley, Kerry |
| author_sort | Inoue, Maiko |
| collection | MIT |
| description | Goldenseal (Hydrastis canadensis L.) is a medicinal plant valued for the treatment of sore eyes and mouths. Although cultivation of the plant has helped meet growing demand, goldenseal is still considered a threatened or endangered species throughout much of its range in North America. In an effort to assess possible conservation strategies for goldenseal genetic resources, levels of genetic diversity within and among cultivated and wild populations were quantified. RAPD analysis was used to examine six cultivated and 11 wild populations sampled from North Carolina, Ohio, Pennsylvania, and West Virginia. The average percentage of polymorphic bands in cultivated and wild populations was low (16.8 and 15.5 %, respectively), and geographic range did not predict the level of genetic diversity. Most of the genetic variation (81.2 %) was within populations; only 3.6 % was partitioned between cultivated and wild populations. Our results differed from a previous study which concluded that genetic differences were greater among than within populations. The results of the current study indicate that, although goldenseal grows clonally and in dense patches, a mixed mating system in which both selfing and outcrossing occur is also operating. We therefore suggest that the ex situ conservation of individual plants within populations, chosen carefully to account for clonal propagation in situ, is an appropriate strategy for sustaining the genetic diversity of goldenseal. |
| first_indexed | 2024-09-23T08:30:16Z |
| format | Article |
| id | mit-1721.1/106863 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:30:16Z |
| publishDate | 2017 |
| publisher | Springer Netherlands |
| record_format | dspace |
| spelling | mit-1721.1/1068632022-09-23T12:45:48Z A measure of genetic diversity of goldenseal (Hydrastis canadensis L.) by RAPD analysis Inoue, Maiko Frary, Amy Craker, Lyle E. Kelley, Kerry Massachusetts Institute of Technology. Department of Biology Kelley, Kerry Jane Goldenseal (Hydrastis canadensis L.) is a medicinal plant valued for the treatment of sore eyes and mouths. Although cultivation of the plant has helped meet growing demand, goldenseal is still considered a threatened or endangered species throughout much of its range in North America. In an effort to assess possible conservation strategies for goldenseal genetic resources, levels of genetic diversity within and among cultivated and wild populations were quantified. RAPD analysis was used to examine six cultivated and 11 wild populations sampled from North Carolina, Ohio, Pennsylvania, and West Virginia. The average percentage of polymorphic bands in cultivated and wild populations was low (16.8 and 15.5 %, respectively), and geographic range did not predict the level of genetic diversity. Most of the genetic variation (81.2 %) was within populations; only 3.6 % was partitioned between cultivated and wild populations. Our results differed from a previous study which concluded that genetic differences were greater among than within populations. The results of the current study indicate that, although goldenseal grows clonally and in dense patches, a mixed mating system in which both selfing and outcrossing occur is also operating. We therefore suggest that the ex situ conservation of individual plants within populations, chosen carefully to account for clonal propagation in situ, is an appropriate strategy for sustaining the genetic diversity of goldenseal. 2017-02-03T23:53:06Z 2017-02-03T23:53:06Z 2013-02 2012-07 2016-08-18T15:19:57Z Article http://purl.org/eprint/type/JournalArticle 0925-9864 1573-5109 http://hdl.handle.net/1721.1/106863 Inoue, Maiko, Kerry J. Kelley, Amy Frary, and Lyle E. Craker. “A Measure of Genetic Diversity of Goldenseal (Hydrastis Canadensis L.) by RAPD Analysis.” Genetic Resources and Crop Evolution 60, no. 3 (February 15, 2013): 1201–1207. en http://dx.doi.org/10.1007/s10722-013-9962-7 Genetic Resources and Crop Evolution Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. Springer Science+Business Media Dordrecht application/pdf Springer Netherlands Springer Netherlands |
| spellingShingle | Inoue, Maiko Frary, Amy Craker, Lyle E. Kelley, Kerry A measure of genetic diversity of goldenseal (Hydrastis canadensis L.) by RAPD analysis |
| title | A measure of genetic diversity of goldenseal (Hydrastis canadensis L.) by RAPD analysis |
| title_full | A measure of genetic diversity of goldenseal (Hydrastis canadensis L.) by RAPD analysis |
| title_fullStr | A measure of genetic diversity of goldenseal (Hydrastis canadensis L.) by RAPD analysis |
| title_full_unstemmed | A measure of genetic diversity of goldenseal (Hydrastis canadensis L.) by RAPD analysis |
| title_short | A measure of genetic diversity of goldenseal (Hydrastis canadensis L.) by RAPD analysis |
| title_sort | measure of genetic diversity of goldenseal hydrastis canadensis l by rapd analysis |
| url | http://hdl.handle.net/1721.1/106863 |
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