Uncharged Helical Modular Polypeptide Hydrogels for Cellular Scaffolds
Grafted synthetic polypeptides hold appeal for extending the range of biophysical properties achievable in synthetic extracellular matrix (ECM) hydrogels. Here, N-carboxyanhydride polypeptide, poly(γ-propargyl-l-glutamate) (PPLG) macromers were generated by fully grafting the “clickable” side chains...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2017
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| Online Access: | http://hdl.handle.net/1721.1/106938 https://orcid.org/0000-0002-1801-5548 |
| _version_ | 1811074653024681984 |
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| author | Ahrens, Caroline C. Welch, M. Elizabeth Griffith, Linda G Hammond, Paula T |
| author2 | Massachusetts Institute of Technology. Center for Gynepathology Research |
| author_facet | Massachusetts Institute of Technology. Center for Gynepathology Research Ahrens, Caroline C. Welch, M. Elizabeth Griffith, Linda G Hammond, Paula T |
| author_sort | Ahrens, Caroline C. |
| collection | MIT |
| description | Grafted synthetic polypeptides hold appeal for extending the range of biophysical properties achievable in synthetic extracellular matrix (ECM) hydrogels. Here, N-carboxyanhydride polypeptide, poly(γ-propargyl-l-glutamate) (PPLG) macromers were generated by fully grafting the “clickable” side chains with mixtures of short polyethylene glycol (PEG) chains terminated with inert (−OH) or reactive (maleimide and/or norbornene) groups, then reacting a fraction of these groups with an RGD cell attachment motif. A panel of synthetic hydrogels was then created by cross-linking the PPLG macromers with a 4-arm PEG star molecule. Compared to well-established PEG-only hydrogels, gels containing PPLG exhibited dramatically less dependence on swelling as a function of cross-link density. Further, PPLG-containing gels, which retain an α-helical chain conformation, were more effective than standard PEG gels in fostering attachment of a human mesenchymal stem cell (hMSC) line for a given concentration of RGD in the gel. These favorable properties of PPLG-containing PEG hydrogels suggest they may find broad use in synthetic ECM. |
| first_indexed | 2024-09-23T09:53:14Z |
| format | Article |
| id | mit-1721.1/106938 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:53:14Z |
| publishDate | 2017 |
| publisher | American Chemical Society (ACS) |
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| spelling | mit-1721.1/1069382022-09-30T17:29:47Z Uncharged Helical Modular Polypeptide Hydrogels for Cellular Scaffolds Ahrens, Caroline C. Welch, M. Elizabeth Griffith, Linda G Hammond, Paula T Massachusetts Institute of Technology. Center for Gynepathology Research Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Hammond, Paula T. Ahrens, Caroline C. Welch, M. Elizabeth Griffith, Linda G Hammond, Paula T Grafted synthetic polypeptides hold appeal for extending the range of biophysical properties achievable in synthetic extracellular matrix (ECM) hydrogels. Here, N-carboxyanhydride polypeptide, poly(γ-propargyl-l-glutamate) (PPLG) macromers were generated by fully grafting the “clickable” side chains with mixtures of short polyethylene glycol (PEG) chains terminated with inert (−OH) or reactive (maleimide and/or norbornene) groups, then reacting a fraction of these groups with an RGD cell attachment motif. A panel of synthetic hydrogels was then created by cross-linking the PPLG macromers with a 4-arm PEG star molecule. Compared to well-established PEG-only hydrogels, gels containing PPLG exhibited dramatically less dependence on swelling as a function of cross-link density. Further, PPLG-containing gels, which retain an α-helical chain conformation, were more effective than standard PEG gels in fostering attachment of a human mesenchymal stem cell (hMSC) line for a given concentration of RGD in the gel. These favorable properties of PPLG-containing PEG hydrogels suggest they may find broad use in synthetic ECM. National Institutes of Health (U.S.) (Grant R01 EB010246-03 and U54-CA112967) National Institutes of Health (U.S.) (Biomechanics Training Grant) 2017-02-15T15:33:04Z 2017-02-15T15:33:04Z 2015-10 2015-08 Article http://purl.org/eprint/type/JournalArticle 1525-7797 1526-4602 http://hdl.handle.net/1721.1/106938 Ahrens, Caroline C. et al. “Uncharged Helical Modular Polypeptide Hydrogels for Cellular Scaffolds.” Biomacromolecules 16.12 (2015): 3774–3783. https://orcid.org/0000-0002-1801-5548 en_US http://dx.doi.org/10.1021/acs.biomac.5b01076 Biomacromolecules Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) Prof. Hammond via Erja Kajosalo |
| spellingShingle | Ahrens, Caroline C. Welch, M. Elizabeth Griffith, Linda G Hammond, Paula T Uncharged Helical Modular Polypeptide Hydrogels for Cellular Scaffolds |
| title | Uncharged Helical Modular Polypeptide Hydrogels for Cellular Scaffolds |
| title_full | Uncharged Helical Modular Polypeptide Hydrogels for Cellular Scaffolds |
| title_fullStr | Uncharged Helical Modular Polypeptide Hydrogels for Cellular Scaffolds |
| title_full_unstemmed | Uncharged Helical Modular Polypeptide Hydrogels for Cellular Scaffolds |
| title_short | Uncharged Helical Modular Polypeptide Hydrogels for Cellular Scaffolds |
| title_sort | uncharged helical modular polypeptide hydrogels for cellular scaffolds |
| url | http://hdl.handle.net/1721.1/106938 https://orcid.org/0000-0002-1801-5548 |
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