Three-dimensional multilayered fibrous constructs for wound healing applications
Electrospun materials are promising scaffolds due to their light-weight, high surface-area and low-cost fabrication, however, such scaffolds are commonly obtained as ultrathin two-dimensional non-woven meshes, lacking on topographical specificity and surface side-dependent properties. Herein, it is...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Brill Academic Publishers
2017
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| Online Access: | http://hdl.handle.net/1721.1/107120 |
| _version_ | 1826209103020556288 |
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| author | Rego, Ana M. B. Aguiar-Ricardo, Ana Reis, Tiago C. Castleberry, Steven A Hammond, Paula T. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Rego, Ana M. B. Aguiar-Ricardo, Ana Reis, Tiago C. Castleberry, Steven A Hammond, Paula T. |
| author_sort | Rego, Ana M. B. |
| collection | MIT |
| description | Electrospun materials are promising scaffolds due to their light-weight, high surface-area and low-cost fabrication, however, such scaffolds are commonly obtained as ultrathin two-dimensional non-woven meshes, lacking on topographical specificity and surface side-dependent properties. Herein, it is reported the production of three-dimensional fibrous materials with an asymmetrical inner structure and engineered surfaces. The manufactured constructs evidence fibrous-based microsized conical protrusions [length: (10 ± 3) × 10[superscript 2] μm; width: (3.8 ± 0.8) × 10[superscript 2] μm] at their top side, with a median peak density of 73 peaks per cm[superscript 2], while their bottom side resembles to a non-woven mesh commonly observed in the fabrication of two-dimensional electrospun materials. Regarding their thickness (3.7 ± 0.1 mm) and asymmetric fibrous inner architecture, such materials avoid external liquid absorption while promoting internal liquid uptake. Nevertheless, such constructs also observed the high porosity (89.9%) and surface area (1.44 m[superscript 2] g[superscript −1]) characteristic of traditional electrospun mats. Spray layer-by-layer assembly is used to effectively coat the structurally complex materials, allowing to complementary tailor features such as water vapor transmission, swelling ratio and bioactive agent release. Tested as wound dressings, the novel constructs are capable of withstanding (11.0 ± 0.3) × 10[superscript 4] kg m[superscript −2] even after 14 days of hydration, while actively promote wound healing (90 ± 0.5% of wound closure within 48 hours) although avoiding cell adhesion on the dressings for a painless removal. |
| first_indexed | 2024-09-23T14:17:36Z |
| format | Article |
| id | mit-1721.1/107120 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:17:36Z |
| publishDate | 2017 |
| publisher | Brill Academic Publishers |
| record_format | dspace |
| spelling | mit-1721.1/1071202022-09-28T19:48:08Z Three-dimensional multilayered fibrous constructs for wound healing applications Rego, Ana M. B. Aguiar-Ricardo, Ana Reis, Tiago C. Castleberry, Steven A Hammond, Paula T. Massachusetts Institute of Technology. Department of Chemical Engineering Reis, Tiago C. Castleberry, Steven A Hammond, Paula T. Electrospun materials are promising scaffolds due to their light-weight, high surface-area and low-cost fabrication, however, such scaffolds are commonly obtained as ultrathin two-dimensional non-woven meshes, lacking on topographical specificity and surface side-dependent properties. Herein, it is reported the production of three-dimensional fibrous materials with an asymmetrical inner structure and engineered surfaces. The manufactured constructs evidence fibrous-based microsized conical protrusions [length: (10 ± 3) × 10[superscript 2] μm; width: (3.8 ± 0.8) × 10[superscript 2] μm] at their top side, with a median peak density of 73 peaks per cm[superscript 2], while their bottom side resembles to a non-woven mesh commonly observed in the fabrication of two-dimensional electrospun materials. Regarding their thickness (3.7 ± 0.1 mm) and asymmetric fibrous inner architecture, such materials avoid external liquid absorption while promoting internal liquid uptake. Nevertheless, such constructs also observed the high porosity (89.9%) and surface area (1.44 m[superscript 2] g[superscript −1]) characteristic of traditional electrospun mats. Spray layer-by-layer assembly is used to effectively coat the structurally complex materials, allowing to complementary tailor features such as water vapor transmission, swelling ratio and bioactive agent release. Tested as wound dressings, the novel constructs are capable of withstanding (11.0 ± 0.3) × 10[superscript 4] kg m[superscript −2] even after 14 days of hydration, while actively promote wound healing (90 ± 0.5% of wound closure within 48 hours) although avoiding cell adhesion on the dressings for a painless removal. Calouste Gulbenkian Foundation Fundação para a Ciência e a Tecnologia (Portugal) (Contracts UID/QUI/50006/2013, MIT-Pt/BS-CTRM/0051/2008, and PTDC/EMETME/ 103375/2008) Fundação para a Ciência e a Tecnologia (Portugal) (Doctoral Grant SFRH/BD/51188/2010) MIT-Portugal Program Fonds Europeen de Developpement Economique et Regional Fonds structurels européens United States. Army Research Office (Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies. Contract W911NF-07-D-0004) Sanofi Aventis (Firm) Massachusetts Institute of Technology. Center for Biomedical Engineering 2017-02-23T16:45:34Z 2017-02-23T16:45:34Z 2016-01 2015-06 Article http://purl.org/eprint/type/JournalArticle 2047-4830 2047-4849 http://hdl.handle.net/1721.1/107120 Reis, Tiago C. et al. “Three-Dimensional Multilayered Fibrous Constructs for Wound Healing Applications.” Biomater. Sci. 4.2 (2016): 319–330. en_US http://dx.doi.org/10.1039/c5bm00211g Biomaterials Science Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Brill Academic Publishers PMC |
| spellingShingle | Rego, Ana M. B. Aguiar-Ricardo, Ana Reis, Tiago C. Castleberry, Steven A Hammond, Paula T. Three-dimensional multilayered fibrous constructs for wound healing applications |
| title | Three-dimensional multilayered fibrous constructs for wound healing applications |
| title_full | Three-dimensional multilayered fibrous constructs for wound healing applications |
| title_fullStr | Three-dimensional multilayered fibrous constructs for wound healing applications |
| title_full_unstemmed | Three-dimensional multilayered fibrous constructs for wound healing applications |
| title_short | Three-dimensional multilayered fibrous constructs for wound healing applications |
| title_sort | three dimensional multilayered fibrous constructs for wound healing applications |
| url | http://hdl.handle.net/1721.1/107120 |
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