Atomic structure of the Y complex of the nuclear pore
available in PMC 2015 November 01
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Springer Nature
2017
|
| Online Access: | http://hdl.handle.net/1721.1/107150 https://orcid.org/0000-0002-0873-803X https://orcid.org/0000-0003-2265-5174 https://orcid.org/0000-0003-4363-441X https://orcid.org/0000-0001-8012-1512 |
| _version_ | 1811097109793865728 |
|---|---|
| author | Kelley, Kotaro Knockenhauer, Kevin Edward Kabachinski, Gregory L. Schwartz, Thomas |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Kelley, Kotaro Knockenhauer, Kevin Edward Kabachinski, Gregory L. Schwartz, Thomas |
| author_sort | Kelley, Kotaro |
| collection | MIT |
| description | available in PMC 2015 November 01 |
| first_indexed | 2024-09-23T16:54:29Z |
| format | Article |
| id | mit-1721.1/107150 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:54:29Z |
| publishDate | 2017 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | mit-1721.1/1071502022-09-29T22:21:51Z Atomic structure of the Y complex of the nuclear pore Kelley, Kotaro Knockenhauer, Kevin Edward Kabachinski, Gregory L. Schwartz, Thomas Massachusetts Institute of Technology. Department of Biology Kelley, Kotaro Knockenhauer, Kevin Edward Kabachinski, Gregory L. Schwartz, Thomas available in PMC 2015 November 01 The nuclear pore complex (NPC) is the principal gateway for transport into and out of the nucleus. Selectivity is achieved through the hydrogel-like core of the NPC. The structural integrity of the NPC depends on ~15 architectural proteins, which are organized in distinct subcomplexes to form the >40-MDa ring-like structure. Here we present the 4.1-Å crystal structure of a heterotetrameric core element ('hub') of the Y complex, the essential NPC building block, from Myceliophthora thermophila. Using the hub structure together with known Y-complex fragments, we built the entire ~0.5-MDa Y complex. Our data reveal that the conserved core of the Y complex has six rather than seven members. Evolutionarily distant Y-complex assemblies share a conserved core that is very similar in shape and dimension, thus suggesting that there are closely related architectural codes for constructing the NPC in all eukaryotes. National Institute of General Medical Sciences (U.S.) (award GM103403) National Institutes of Health (U.S.) National Science Foundation (U.S.) (NSF Graduate Research Fellowship Program, grant 1122374) National Institutes of Health (U.S.) (NIH Grant R01GM77537) National Institutes of Health (U.S.) (NIH Grant T32GM007287) 2017-02-23T21:52:32Z 2017-02-23T21:52:32Z 2015-03 2015-01 Article http://purl.org/eprint/type/JournalArticle 1545-9993 1545-9985 http://hdl.handle.net/1721.1/107150 Kelley, Kotaro, Kevin E Knockenhauer, Greg Kabachinski, and Thomas U Schwartz. “Atomic Structure of the Y Complex of the Nuclear Pore.” Nature Structural & Molecular Biology (March 30, 2015). https://orcid.org/0000-0002-0873-803X https://orcid.org/0000-0003-2265-5174 https://orcid.org/0000-0003-4363-441X https://orcid.org/0000-0001-8012-1512 en_US http://dx.doi.org/10.1038/nsmb.2998 Nature Structural & Molecular Biology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Springer Nature PMC |
| spellingShingle | Kelley, Kotaro Knockenhauer, Kevin Edward Kabachinski, Gregory L. Schwartz, Thomas Atomic structure of the Y complex of the nuclear pore |
| title | Atomic structure of the Y complex of the nuclear pore |
| title_full | Atomic structure of the Y complex of the nuclear pore |
| title_fullStr | Atomic structure of the Y complex of the nuclear pore |
| title_full_unstemmed | Atomic structure of the Y complex of the nuclear pore |
| title_short | Atomic structure of the Y complex of the nuclear pore |
| title_sort | atomic structure of the y complex of the nuclear pore |
| url | http://hdl.handle.net/1721.1/107150 https://orcid.org/0000-0002-0873-803X https://orcid.org/0000-0003-2265-5174 https://orcid.org/0000-0003-4363-441X https://orcid.org/0000-0001-8012-1512 |
| work_keys_str_mv | AT kelleykotaro atomicstructureoftheycomplexofthenuclearpore AT knockenhauerkevinedward atomicstructureoftheycomplexofthenuclearpore AT kabachinskigregoryl atomicstructureoftheycomplexofthenuclearpore AT schwartzthomas atomicstructureoftheycomplexofthenuclearpore |