Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy
Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical re...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/107616 https://orcid.org/0000-0003-2728-0746 https://orcid.org/0000-0003-1970-9901 https://orcid.org/0000-0002-4942-3456 https://orcid.org/0000-0002-7232-304X |
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| author | Scarcelli, Giuliano Patel, Kripa Polacheck III, William Joseph Tavakoli Nia, Hadi Grodzinsky, Alan J Kamm, Roger Dale Yun, Seok Hyun (Andy) |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Scarcelli, Giuliano Patel, Kripa Polacheck III, William Joseph Tavakoli Nia, Hadi Grodzinsky, Alan J Kamm, Roger Dale Yun, Seok Hyun (Andy) |
| author_sort | Scarcelli, Giuliano |
| collection | MIT |
| description | Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation. |
| first_indexed | 2024-09-23T12:00:42Z |
| format | Article |
| id | mit-1721.1/107616 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:00:42Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1076162022-10-01T07:36:57Z Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy Scarcelli, Giuliano Patel, Kripa Polacheck III, William Joseph Tavakoli Nia, Hadi Grodzinsky, Alan J Kamm, Roger Dale Yun, Seok Hyun (Andy) Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Mechanical Engineering Polacheck III, William Joseph Tavakoli Nia, Hadi Grodzinsky, Alan J Kamm, Roger Dale Yun, Seok Hyun (Andy) Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation. National Institutes of Health (U.S.) (Grants P41-EB015903 and R33 CA174550-01) National Science Foundation (U.S.) (Grant CBET-0853773) 2017-03-21T14:16:22Z 2017-03-21T14:16:22Z 2015-10 2015-03 Article http://purl.org/eprint/type/JournalArticle 1548-7091 1548-7105 http://hdl.handle.net/1721.1/107616 Scarcelli, Giuliano et al. “Noncontact Three-Dimensional Mapping of Intracellular Hydromechanical Properties by Brillouin Microscopy.” Nature Methods 12.12 (2015): 1132–1134. https://orcid.org/0000-0003-2728-0746 https://orcid.org/0000-0003-1970-9901 https://orcid.org/0000-0002-4942-3456 https://orcid.org/0000-0002-7232-304X en_US http://dx.doi.org/10.1038/nmeth.3616 Nature Methods Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Scarcelli, Giuliano Patel, Kripa Polacheck III, William Joseph Tavakoli Nia, Hadi Grodzinsky, Alan J Kamm, Roger Dale Yun, Seok Hyun (Andy) Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy |
| title | Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy |
| title_full | Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy |
| title_fullStr | Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy |
| title_full_unstemmed | Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy |
| title_short | Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy |
| title_sort | noncontact three dimensional mapping of intracellular hydromechanical properties by brillouin microscopy |
| url | http://hdl.handle.net/1721.1/107616 https://orcid.org/0000-0003-2728-0746 https://orcid.org/0000-0003-1970-9901 https://orcid.org/0000-0002-4942-3456 https://orcid.org/0000-0002-7232-304X |
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