Molecular imaging with engineered physiology
In vivo imaging techniques are powerful tools for evaluating biological systems. Relating image signals to precise molecular phenomena can be challenging, however, due to limitations of the existing optical, magnetic and radioactive imaging probe mechanisms. Here we demonstrate a concept for molecul...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/107640 https://orcid.org/0000-0002-7472-5480 https://orcid.org/0000-0001-9307-9878 https://orcid.org/0000-0002-2834-6359 |
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| author | Desai, Mitul Slusarczyk, Adrian Lukas Chapin, Ashley A. Barch, Mariya Jasanoff, Alan Pradip |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Desai, Mitul Slusarczyk, Adrian Lukas Chapin, Ashley A. Barch, Mariya Jasanoff, Alan Pradip |
| author_sort | Desai, Mitul |
| collection | MIT |
| description | In vivo imaging techniques are powerful tools for evaluating biological systems. Relating image signals to precise molecular phenomena can be challenging, however, due to limitations of the existing optical, magnetic and radioactive imaging probe mechanisms. Here we demonstrate a concept for molecular imaging which bypasses the need for conventional imaging agents by perturbing the endogenous multimodal contrast provided by the vasculature. Variants of the calcitonin gene-related peptide artificially activate vasodilation pathways in rat brain and induce contrast changes that are readily measured by optical and magnetic resonance imaging. CGRP-based agents induce effects at nanomolar concentrations in deep tissue and can be engineered into switchable analyte-dependent forms and genetically encoded reporters suitable for molecular imaging or cell tracking. Such artificially engineered physiological changes, therefore, provide a highly versatile means for sensitive analysis of molecular events in living organisms. |
| first_indexed | 2024-09-23T16:01:44Z |
| format | Article |
| id | mit-1721.1/107640 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:01:44Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1076402022-10-02T05:47:50Z Molecular imaging with engineered physiology Desai, Mitul Slusarczyk, Adrian Lukas Chapin, Ashley A. Barch, Mariya Jasanoff, Alan Pradip Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Nuclear Science and Engineering Desai, Mitul Slusarczyk, Adrian Lukas Chapin, Ashley A. Barch, Mariya Jasanoff, Alan Pradip In vivo imaging techniques are powerful tools for evaluating biological systems. Relating image signals to precise molecular phenomena can be challenging, however, due to limitations of the existing optical, magnetic and radioactive imaging probe mechanisms. Here we demonstrate a concept for molecular imaging which bypasses the need for conventional imaging agents by perturbing the endogenous multimodal contrast provided by the vasculature. Variants of the calcitonin gene-related peptide artificially activate vasodilation pathways in rat brain and induce contrast changes that are readily measured by optical and magnetic resonance imaging. CGRP-based agents induce effects at nanomolar concentrations in deep tissue and can be engineered into switchable analyte-dependent forms and genetically encoded reporters suitable for molecular imaging or cell tracking. Such artificially engineered physiological changes, therefore, provide a highly versatile means for sensitive analysis of molecular events in living organisms. National Institute of Mental Health (U.S.) (R01-MH103160) National Institute of Mental Health (U.S.) (R01-NS076462) BRAIN Initiative (award R24-MH109081) Massachusetts Institute of Technology. Simons Center for the Social Brain Boehringer Ingelheim Fonds (predoctoral fellowships) McGovern Institute for Brain Research at MIT 2017-03-22T15:28:19Z 2017-03-22T15:28:19Z 2016-12 2016-03 Article http://purl.org/eprint/type/JournalArticle 2041-1723 http://hdl.handle.net/1721.1/107640 Desai, Mitul, Adrian L. Slusarczyk, Ashley Chapin, Mariya Barch, and Alan Jasanoff. “Molecular Imaging with Engineered Physiology.” Nature Communications 7 (December 2, 2016): 13607. doi:10.1038/ncomms13607. https://orcid.org/0000-0002-7472-5480 https://orcid.org/0000-0001-9307-9878 https://orcid.org/0000-0002-2834-6359 en_US http://dx.doi.org/10.1038/ncomms13607 Nature Communications Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature |
| spellingShingle | Desai, Mitul Slusarczyk, Adrian Lukas Chapin, Ashley A. Barch, Mariya Jasanoff, Alan Pradip Molecular imaging with engineered physiology |
| title | Molecular imaging with engineered physiology |
| title_full | Molecular imaging with engineered physiology |
| title_fullStr | Molecular imaging with engineered physiology |
| title_full_unstemmed | Molecular imaging with engineered physiology |
| title_short | Molecular imaging with engineered physiology |
| title_sort | molecular imaging with engineered physiology |
| url | http://hdl.handle.net/1721.1/107640 https://orcid.org/0000-0002-7472-5480 https://orcid.org/0000-0001-9307-9878 https://orcid.org/0000-0002-2834-6359 |
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