Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion
Improved biomaterials are required for application in regenerative medicine, biosensing, and as medical devices. The response of cells to the chemistry of polymers cultured in media is generally regarded as being dominated by proteins adsorbed to the surface. Here we use mass spectrometry to identif...
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| Format: | Article |
| Language: | en_US |
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Royal Society of Chemistry
2017
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| Online Access: | http://hdl.handle.net/1721.1/107663 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 |
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| author | Hammad, Moamen Rao, Wei Smith, James G. W. Young, Lorraine E. Barrett, David A. Davies, Martyn C. Denning, Chris Alexander, Morgan R. Anderson, Daniel Griffith Langer, Robert S |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Hammad, Moamen Rao, Wei Smith, James G. W. Young, Lorraine E. Barrett, David A. Davies, Martyn C. Denning, Chris Alexander, Morgan R. Anderson, Daniel Griffith Langer, Robert S |
| author_sort | Hammad, Moamen |
| collection | MIT |
| description | Improved biomaterials are required for application in regenerative medicine, biosensing, and as medical devices. The response of cells to the chemistry of polymers cultured in media is generally regarded as being dominated by proteins adsorbed to the surface. Here we use mass spectrometry to identify proteins adsorbed from a complex mouse embryonic fibroblast (MEF) conditioned medium found to support pluripotent human embryonic stem cell (hESC) expansion on a plasma etched tissue culture polystyrene surface. A total of 71 proteins were identified, of which 14 uniquely correlated with the surface on which pluripotent stem cell expansion was achieved. We have developed a microarray combinatorial protein spotting approach to test the potential of these 14 proteins to support expansion of a hESC cell line (HUES-7) and a human induced pluripotent stem cell line (ReBl-PAT) on a novel polymer (N-(4-Hydroxyphenyl) methacrylamide). These proteins were spotted to form a primary array yielding several protein mixture ‘hits’ that enhanced cell attachment to the polymer. A second array was generated to test the function of a refined set of protein mixtures. We found that a combination of heat shock protein 90 and heat shock protein-1 encourage elevated adherence of pluripotent stem cells at a level comparable to fibronectin pre-treatment. |
| first_indexed | 2024-09-23T15:16:48Z |
| format | Article |
| id | mit-1721.1/107663 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:16:48Z |
| publishDate | 2017 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/1076632022-10-02T01:54:07Z Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion Hammad, Moamen Rao, Wei Smith, James G. W. Young, Lorraine E. Barrett, David A. Davies, Martyn C. Denning, Chris Alexander, Morgan R. Anderson, Daniel Griffith Langer, Robert S Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Anderson, Daniel Griffith Langer, Robert S Improved biomaterials are required for application in regenerative medicine, biosensing, and as medical devices. The response of cells to the chemistry of polymers cultured in media is generally regarded as being dominated by proteins adsorbed to the surface. Here we use mass spectrometry to identify proteins adsorbed from a complex mouse embryonic fibroblast (MEF) conditioned medium found to support pluripotent human embryonic stem cell (hESC) expansion on a plasma etched tissue culture polystyrene surface. A total of 71 proteins were identified, of which 14 uniquely correlated with the surface on which pluripotent stem cell expansion was achieved. We have developed a microarray combinatorial protein spotting approach to test the potential of these 14 proteins to support expansion of a hESC cell line (HUES-7) and a human induced pluripotent stem cell line (ReBl-PAT) on a novel polymer (N-(4-Hydroxyphenyl) methacrylamide). These proteins were spotted to form a primary array yielding several protein mixture ‘hits’ that enhanced cell attachment to the polymer. A second array was generated to test the function of a refined set of protein mixtures. We found that a combination of heat shock protein 90 and heat shock protein-1 encourage elevated adherence of pluripotent stem cells at a level comparable to fibronectin pre-treatment. Engineering and Physical Sciences Research Council (Grant H045384) Royal Pharmaceutical Society of Great Britain 2017-03-23T17:31:02Z 2017-03-23T17:31:02Z 2016-07 2016-03 Article http://purl.org/eprint/type/JournalArticle 2047-4830 2047-4849 http://hdl.handle.net/1721.1/107663 Hammad, Moamen et al. “Identification of Polymer Surface Adsorbed Proteins Implicated in Pluripotent Human Embryonic Stem Cell Expansion.” Biomater. Sci. 4.9 (2016): 1381–1391. © The Royal Society of Chemistry 2016 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.1039/c6bm00214e Biomaterials Science Creative Commons Attribution 3.0 License http://creativecommons.org/licenses/by/3.0/ application/pdf Royal Society of Chemistry Royal Society of Chemistry |
| spellingShingle | Hammad, Moamen Rao, Wei Smith, James G. W. Young, Lorraine E. Barrett, David A. Davies, Martyn C. Denning, Chris Alexander, Morgan R. Anderson, Daniel Griffith Langer, Robert S Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion |
| title | Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion |
| title_full | Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion |
| title_fullStr | Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion |
| title_full_unstemmed | Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion |
| title_short | Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion |
| title_sort | identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion |
| url | http://hdl.handle.net/1721.1/107663 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 |
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