Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy
Neuronal plasticity helps animals learn from their environment. However, it is challenging to link specific changes in defined neurons to altered behavior. Here, we focus on circadian rhythms in the structure of the principal s-LNv clock neurons in Drosophila. By quantifying neuronal architecture, w...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2017
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| Online Access: | http://hdl.handle.net/1721.1/107702 https://orcid.org/0000-0003-0302-0691 |
| _version_ | 1826205392550494208 |
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| author | Petsakou, Afroditi Blau, Justin Sapsis, Themistoklis P. |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Petsakou, Afroditi Blau, Justin Sapsis, Themistoklis P. |
| author_sort | Petsakou, Afroditi |
| collection | MIT |
| description | Neuronal plasticity helps animals learn from their environment. However, it is challenging to link specific changes in defined neurons to altered behavior. Here, we focus on circadian rhythms in the structure of the principal s-LNv clock neurons in Drosophila. By quantifying neuronal architecture, we observed that s-LNv structural plasticity changes the amount of axonal material in addition to cycles of fasciculation and defasciculation. We found that this is controlled by rhythmic Rho1 activity that retracts s-LNv axonal termini by increasing myosin phosphorylation and simultaneously changes the balance of pre-synaptic and dendritic markers. This plasticity is required to change clock network hierarchy and allow seasonal adaptation. Rhythms in Rho1 activity are controlled by clock-regulated transcription of Puratrophin-1-like (Pura), a Rho1 GEF. Since spinocerebellar ataxia is associated with mutations in human Puratrophin-1, our data support the idea that defective actin-related plasticity underlies this ataxia. |
| first_indexed | 2024-09-23T13:12:16Z |
| format | Article |
| id | mit-1721.1/107702 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:12:16Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1077022022-09-28T12:37:44Z Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy Petsakou, Afroditi Blau, Justin Sapsis, Themistoklis P. Massachusetts Institute of Technology. Department of Mechanical Engineering Sapsis, Themistoklis P. Neuronal plasticity helps animals learn from their environment. However, it is challenging to link specific changes in defined neurons to altered behavior. Here, we focus on circadian rhythms in the structure of the principal s-LNv clock neurons in Drosophila. By quantifying neuronal architecture, we observed that s-LNv structural plasticity changes the amount of axonal material in addition to cycles of fasciculation and defasciculation. We found that this is controlled by rhythmic Rho1 activity that retracts s-LNv axonal termini by increasing myosin phosphorylation and simultaneously changes the balance of pre-synaptic and dendritic markers. This plasticity is required to change clock network hierarchy and allow seasonal adaptation. Rhythms in Rho1 activity are controlled by clock-regulated transcription of Puratrophin-1-like (Pura), a Rho1 GEF. Since spinocerebellar ataxia is associated with mutations in human Puratrophin-1, our data support the idea that defective actin-related plasticity underlies this ataxia. Courant Institute of Mathematical Sciences (Postdoctoral Fellowship) 2017-03-24T20:44:11Z 2017-03-24T20:44:11Z 2015-07 2015-03 Article http://purl.org/eprint/type/JournalArticle 0092-8674 http://hdl.handle.net/1721.1/107702 Petsakou, Afroditi, Themistoklis P. Sapsis, and Justin Blau. “Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy.” Cell 162.4 (2015): 823–835. © 2017 Elsevier https://orcid.org/0000-0003-0302-0691 en_US http://dx.doi.org/10.1016/j.cell.2015.07.010 Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Petsakou, Afroditi Blau, Justin Sapsis, Themistoklis P. Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy |
| title | Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy |
| title_full | Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy |
| title_fullStr | Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy |
| title_full_unstemmed | Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy |
| title_short | Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy |
| title_sort | circadian rhythms in rho1 activity regulate neuronal plasticity and network hierarchy |
| url | http://hdl.handle.net/1721.1/107702 https://orcid.org/0000-0003-0302-0691 |
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