A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians
Regeneration starts with injury. Yet how injuries affect gene expression in different cell types and how distinct injuries differ in gene expression remain unclear. We defined the transcriptomes of major cell types of planarians—flatworms that regenerate from nearly any injury—and identified 1,214 t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier B.V.
2017
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| Online Access: | http://hdl.handle.net/1721.1/107733 https://orcid.org/0000-0002-1772-7447 https://orcid.org/0000-0001-8567-2049 https://orcid.org/0000-0002-5569-333X |
| _version_ | 1826212168381497344 |
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| author | Wurtzel, Omri Poirier, Amber Satija, Rahul Cote, Lauren Esther Regev, Aviv Reddien, Peter |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Wurtzel, Omri Poirier, Amber Satija, Rahul Cote, Lauren Esther Regev, Aviv Reddien, Peter |
| author_sort | Wurtzel, Omri |
| collection | MIT |
| description | Regeneration starts with injury. Yet how injuries affect gene expression in different cell types and how distinct injuries differ in gene expression remain unclear. We defined the transcriptomes of major cell types of planarians—flatworms that regenerate from nearly any injury—and identified 1,214 tissue-specific markers across 13 cell types. RNA sequencing on 619 single cells revealed that wound-induced genes were expressed either in nearly all cell types or specifically in one of three cell types (stem cells, muscle, or epidermis). Time course experiments following different injuries indicated that a generic wound response is activated with any injury regardless of the regenerative outcome. Only one gene, notum, was differentially expressed early between anterior- and posterior-facing wounds. Injury-specific transcriptional responses emerged 30 hr after injury, involving context-dependent patterning and stem-cell-specialization genes. The regenerative requirement of every injury is different; however, our work demonstrates that all injuries start with a common transcriptional response. |
| first_indexed | 2024-09-23T15:17:27Z |
| format | Article |
| id | mit-1721.1/107733 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:17:27Z |
| publishDate | 2017 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | mit-1721.1/1077332022-10-02T01:58:46Z A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians Wurtzel, Omri Poirier, Amber Satija, Rahul Cote, Lauren Esther Regev, Aviv Reddien, Peter Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Cote, Lauren Esther Regev, Aviv Reddien, Peter Regeneration starts with injury. Yet how injuries affect gene expression in different cell types and how distinct injuries differ in gene expression remain unclear. We defined the transcriptomes of major cell types of planarians—flatworms that regenerate from nearly any injury—and identified 1,214 tissue-specific markers across 13 cell types. RNA sequencing on 619 single cells revealed that wound-induced genes were expressed either in nearly all cell types or specifically in one of three cell types (stem cells, muscle, or epidermis). Time course experiments following different injuries indicated that a generic wound response is activated with any injury regardless of the regenerative outcome. Only one gene, notum, was differentially expressed early between anterior- and posterior-facing wounds. Injury-specific transcriptional responses emerged 30 hr after injury, involving context-dependent patterning and stem-cell-specialization genes. The regenerative requirement of every injury is different; however, our work demonstrates that all injuries start with a common transcriptional response. Broad Institute of MIT and Harvard. Klarman Cell Observatory Howard Hughes Medical Institute (Investigator) National Institutes of Health (U.S.) (NIH grant R01GM080639) European Molecular Biology Organization (Fellowship) National Institutes of Health (U.S.) (NIH grant F32 HD075541) 2017-03-27T19:08:40Z 2017-03-27T19:08:40Z 2015-12 2015-10 Article http://purl.org/eprint/type/JournalArticle 15345807 http://hdl.handle.net/1721.1/107733 Wurtzel, Omri, Lauren E. Cote, Amber Poirier, Rahul Satija, Aviv Regev, and Peter W. Reddien. “A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians.” Developmental Cell 35, no. 5 (December 2015): 632–645. https://orcid.org/0000-0002-1772-7447 https://orcid.org/0000-0001-8567-2049 https://orcid.org/0000-0002-5569-333X en_US http://dx.doi.org/10.1016/j.devcel.2015.11.004 Developmental Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier B.V. Elsevier |
| spellingShingle | Wurtzel, Omri Poirier, Amber Satija, Rahul Cote, Lauren Esther Regev, Aviv Reddien, Peter A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians |
| title | A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians |
| title_full | A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians |
| title_fullStr | A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians |
| title_full_unstemmed | A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians |
| title_short | A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians |
| title_sort | generic and cell type specific wound response precedes regeneration in planarians |
| url | http://hdl.handle.net/1721.1/107733 https://orcid.org/0000-0002-1772-7447 https://orcid.org/0000-0001-8567-2049 https://orcid.org/0000-0002-5569-333X |
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